##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620314
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9181446815645	32.0	32.0	32.0	32.0	32.0
2	30.73892254567848	32.0	32.0	32.0	32.0	32.0
3	31.187783928784455	32.0	32.0	32.0	32.0	32.0
4	31.45378308405098	32.0	32.0	32.0	32.0	32.0
5	31.28705139655078	32.0	32.0	32.0	32.0	32.0
6	34.953231750371586	36.0	36.0	36.0	36.0	36.0
7	34.96561096476945	36.0	36.0	36.0	36.0	36.0
8	34.864990956193154	36.0	36.0	36.0	32.0	36.0
9	35.09577891197039	36.0	36.0	36.0	36.0	36.0
10	34.85444468446625	36.0	36.0	36.0	32.0	36.0
11	35.11431952204851	36.0	36.0	36.0	36.0	36.0
12	34.95845652363158	36.0	36.0	36.0	32.0	36.0
13	35.05445951566465	36.0	36.0	36.0	36.0	36.0
14	34.99623900153793	36.0	36.0	36.0	32.0	36.0
15	34.926866071054334	36.0	36.0	36.0	32.0	36.0
16	34.95764886815387	36.0	36.0	36.0	32.0	36.0
17	34.9280154889298	36.0	36.0	36.0	32.0	36.0
18	34.95576433870588	36.0	36.0	36.0	32.0	36.0
19	34.95531295440696	36.0	36.0	36.0	32.0	36.0
20	34.93355784328582	36.0	36.0	36.0	32.0	36.0
21	34.94683176584762	36.0	36.0	36.0	32.0	36.0
22	34.9284862182701	36.0	36.0	36.0	32.0	36.0
23	34.890479660301075	36.0	36.0	36.0	32.0	36.0
24	34.86854238337358	36.0	36.0	36.0	32.0	36.0
25	34.85172670615204	36.0	36.0	36.0	32.0	36.0
26	34.80270637128938	36.0	36.0	36.0	32.0	36.0
27	34.780846474527415	36.0	36.0	36.0	32.0	36.0
28	34.75443888095384	36.0	36.0	36.0	32.0	36.0
29	34.72281135038062	36.0	36.0	36.0	32.0	36.0
30	34.719720980019794	36.0	36.0	36.0	32.0	36.0
31	34.72604358437823	36.0	36.0	36.0	32.0	36.0
32	34.692412229935165	36.0	36.0	36.0	32.0	36.0
33	34.6600060614463	36.0	36.0	36.0	32.0	36.0
34	34.6611345221936	36.0	36.0	36.0	32.0	36.0
35	34.65095580625296	36.0	36.0	36.0	32.0	36.0
36	34.60516286912757	36.0	36.0	36.0	32.0	36.0
37	34.61100345953823	36.0	36.0	36.0	32.0	36.0
38	34.570983405178666	36.0	36.0	36.0	32.0	36.0
39	34.54905257659831	36.0	36.0	36.0	32.0	36.0
40	34.55985194594995	36.0	36.0	36.0	32.0	36.0
41	34.529588240794176	36.0	36.0	36.0	32.0	36.0
42	34.505879280493424	36.0	36.0	36.0	32.0	36.0
43	34.50528280838414	36.0	36.0	36.0	32.0	36.0
44	34.45912553964605	36.0	36.0	36.0	32.0	36.0
45	34.42773014956941	36.0	36.0	36.0	32.0	36.0
46	34.41729672391724	36.0	36.0	36.0	32.0	36.0
47	34.42106417072644	36.0	36.0	36.0	32.0	36.0
48	34.3689051028995	36.0	36.0	36.0	32.0	36.0
49	34.34191554599767	36.0	36.0	36.0	32.0	36.0
50	33.74988151162153	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	13.0
22	29.0
23	106.0
24	242.0
25	614.0
26	1282.0
27	2587.0
28	4671.0
29	8202.0
30	13647.0
31	22387.0
32	36816.0
33	68615.0
34	152523.0
35	308576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64309034187552	18.393080265374724	11.673102191840453	25.290727200909302
2	16.568836239764885	20.43057210244869	37.767596176633226	25.232995481153203
3	18.707873953549107	24.252189627461064	29.66108935679038	27.378847062199448
4	12.657782993774122	16.15359318022808	37.201320621491696	33.987303204506105
5	14.744145706851691	36.18248177535893	33.86913724339608	15.204235274393291
6	33.48207592643045	36.81895049418759	16.82027241174185	12.878701167640102
7	29.620482529815543	30.833739041840104	21.176049549099325	18.369728879245027
8	27.246201117498558	33.64392871997086	19.977140609433285	19.132729553097302
9	27.101274515809738	13.910696840632324	19.143852951892107	39.84417569166583
10	15.602904335546192	26.665688667352338	32.11357473795529	25.617832259146173
11	37.46054417601408	21.309852751993343	22.86293715763307	18.3666659143595
12	23.966088142456883	24.033312161260266	29.634991310852243	22.365608385430605
13	28.802026070667434	19.722753315256465	25.985226836731073	25.48999377734502
14	22.792650173944164	20.642126406948737	25.47338928349191	31.091834135615187
15	24.509038970585863	28.328556182836433	22.521658385914233	24.640746460663472
16	25.09261438561761	26.450636290652795	24.715869704697944	23.740879619031652
17	23.208084937628364	26.691643264540215	25.903494036890994	24.196777760940428
18	24.071357409312057	25.65474904645067	27.147863823805363	23.12602972043191
19	24.839033134831716	25.60815973845504	25.988773427651157	23.564033699062087
20	24.914478796222557	25.418900750265188	25.428412062278138	24.238208391234117
21	26.40453060869173	25.041027608598228	24.761814177980828	23.79262760472922
22	25.204654416956572	25.489510151310462	25.515948374532897	23.789887057200062
23	23.867912057441877	25.194337061552694	26.170294399288103	24.767456481717325
24	24.352827761424052	25.87205834464481	25.89430514223442	23.880808751696723
25	24.41247497235271	25.366185512498507	25.97845607224728	24.242883442901498
26	23.66640120970992	26.481588356864428	26.452087168756467	23.399923264669184
27	24.630751522615967	25.711494501171988	25.334588611574137	24.32316536463791
28	23.622552449243447	25.84932792102064	26.003765834722415	24.524353795013493
29	23.916919495610287	25.582043932588977	25.853680555331653	24.64735601646908
30	23.599015982228355	25.965075751957876	26.35052570149956	24.0853825643142
31	24.448746924944466	25.63298587489562	25.136140728727707	24.78212647143221
32	24.101987058167314	26.04068262202691	25.00314356922462	24.85418675058116
33	23.728788968167734	25.62669873644638	25.96330245649784	24.681209838888048
34	24.587870014218606	25.51417507907286	26.072440731629463	23.825514175079075
35	25.059244189233194	25.50498618441628	25.871897135966627	23.563872490383904
36	23.59192280038819	26.285397395512593	25.545610771319044	24.577069032780173
37	24.708131688145034	25.94621433661017	25.285581173405724	24.06007280183907
38	23.977856375964432	25.697146928813474	25.78452203239005	24.54047466283205
39	24.90641836231328	25.20159145207105	24.901259684611343	24.99073050100433
40	25.06375803222239	25.70343406726271	25.852229677227985	23.380578223286914
41	23.807297594444105	25.870768675219324	26.465467489045867	23.856466241290704
42	25.168866090399376	26.299744967871106	25.40132900434296	23.130059937386548
43	24.02766985752377	25.481610926079373	25.952179057703034	24.538540158693824
44	23.788274970418207	25.759212269914915	25.480804882688446	24.97170787697843
45	23.960607047398575	26.194153283659567	25.607353695064113	24.237885973877745
46	23.96157429946769	25.565439438735865	25.340230915310634	25.132755346485812
47	24.322198112568795	25.20110782603649	26.053740524959938	24.422953536434775
48	25.017652350261322	26.056158655132723	24.760040882520787	24.16614811208517
49	23.98333747102274	25.879473943841347	25.36683034721125	24.770358237924665
50	23.97608308050439	26.183674719577503	25.258820532826924	24.58142166709118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.0
2	6.0
3	11.5
4	17.0
5	22.5
6	28.0
7	44.5
8	61.0
9	87.0
10	113.0
11	148.5
12	184.0
13	269.5
14	355.0
15	489.5
16	624.0
17	830.5
18	1037.0
19	1296.5
20	1556.0
21	2009.0
22	2462.0
23	2963.5
24	3465.0
25	4439.0
26	5413.0
27	7086.0
28	8759.0
29	10093.5
30	11428.0
31	12253.0
32	13078.0
33	15057.0
34	17036.0
35	19777.5
36	22519.0
37	25930.5
38	29342.0
39	30759.0
40	32176.0
41	33980.0
42	35784.0
43	35675.0
44	35566.0
45	37986.5
46	40407.0
47	42583.0
48	44759.0
49	45815.5
50	46872.0
51	44132.5
52	41393.0
53	40362.0
54	39331.0
55	39569.5
56	39808.0
57	38827.0
58	37846.0
59	34098.5
60	30351.0
61	26604.5
62	22858.0
63	19798.5
64	16739.0
65	14331.5
66	11924.0
67	10162.5
68	8401.0
69	7769.0
70	7137.0
71	5507.0
72	3877.0
73	3493.0
74	3109.0
75	2259.5
76	1410.0
77	1251.0
78	1092.0
79	940.5
80	789.0
81	622.5
82	456.0
83	380.5
84	305.0
85	264.5
86	224.0
87	174.5
88	125.0
89	89.0
90	53.0
91	38.0
92	23.0
93	16.5
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00951131201294828
2	0.0037077995982679737
3	4.8362603455669227E-4
4	0.0
5	0.0
6	4.8362603455669227E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	620314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.107199374743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.46496761527804	55.43026917843328
2	9.0059907997906	11.546976955385546
3	2.0740336357729325	3.9888146339525545
4	0.754969473608152	1.9359591426577034
5	0.3749550153883026	1.2018657964028874
6	0.2434135803459254	0.9362737755453759
7	0.16016115670297026	0.718723824339274
8	0.1084606592484655	0.5562487285405954
9	0.07974114338008811	0.460078323933355
>10	0.556718155881998	7.237663439999663
>50	0.08778986619014335	4.023184030275992
>100	0.08451031525048745	10.093939422178648
>500	0.004288583161961037	1.8700027483552115
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	996	0.16056384347282182	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	952	0.15347066163265702	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	873	0.14073517605599745	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	792	0.12767727312296676	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	754	0.12155134335191532	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	734	0.11832716978820404	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	679	0.109460692487998	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	676	0.10897706645344132	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	642	0.10349597139513214	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	638	0.10285113668238989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6120867818556408E-4
2	0.0	0.0	0.0	0.0	1.6120867818556408E-4
3	0.0	0.0	0.0	0.0	1.6120867818556408E-4
4	0.0	0.0	0.0	0.0	1.6120867818556408E-4
5	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
6	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
7	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
8	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
9	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
10	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
11	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
12	0.0	0.0	0.0	1.6120867818556408E-4	1.6120867818556408E-4
13	0.0	0.0	0.0	1.6120867818556408E-4	3.2241735637112816E-4
14	0.0	0.0	0.0	1.6120867818556408E-4	3.2241735637112816E-4
15	0.0	0.0	0.0	1.6120867818556408E-4	4.8362603455669227E-4
16	0.0	0.0	0.0	1.6120867818556408E-4	4.8362603455669227E-4
17	0.0	0.0	0.0	1.6120867818556408E-4	4.8362603455669227E-4
18	0.0	0.0	0.0	1.6120867818556408E-4	4.8362603455669227E-4
19	0.0	0.0	0.0	1.6120867818556408E-4	4.8362603455669227E-4
20	0.0	0.0	0.0	1.6120867818556408E-4	6.448347127422563E-4
21	0.0	0.0	0.0	1.6120867818556408E-4	6.448347127422563E-4
22	0.0	0.0	0.0	1.6120867818556408E-4	8.060433909278204E-4
23	0.0	0.0	0.0	1.6120867818556408E-4	8.060433909278204E-4
24	0.0	0.0	0.0	3.2241735637112816E-4	8.060433909278204E-4
25	0.0	0.0	0.0	3.2241735637112816E-4	8.060433909278204E-4
26	0.0	0.0	0.0	3.2241735637112816E-4	0.0011284607472989486
27	0.0	0.0	0.0	8.060433909278204E-4	0.0011284607472989486
28	0.0	0.0	0.0	0.001773295460041205	0.0011284607472989486
29	0.0	0.0	0.0	0.0025793388509690253	0.0011284607472989486
30	0.0	0.0	0.0	0.004675051667381359	0.0011284607472989486
31	0.0	0.0	0.0	0.010156146725690537	0.0011284607472989486
32	0.0	0.0	0.0	0.019506250060453253	0.0011284607472989486
33	0.0	0.0	0.0	0.03256415299348395	0.0011284607472989486
34	0.0	0.0	0.0	0.04723414270837028	0.0011284607472989486
35	0.0	0.0	0.0	0.06448347127422563	0.0011284607472989486
36	0.0	0.0	0.0	0.09575795484222506	0.0011284607472989486
37	0.0	0.0	0.0	0.1476671492179767	0.0011284607472989486
38	0.0	0.0	0.0	0.22343522796519183	0.0011284607472989486
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	25	0.0023491872	35.208317	1
CGCGGGA	60	2.8707014E-4	21.99988	44
TACCCCG	50	0.0025791177	21.999878	5
GGTCGTA	80	3.6110196E-6	21.999878	29
TTGTCGA	70	3.2122356E-5	21.999878	44
GTATCAA	910	0.0	21.521568	1
ATTGTCG	75	5.467364E-5	20.53322	43
GTCCTAA	215	0.0	20.469954	1
TTGTACT	140	1.8371793E-10	20.42846	4
GTATTGA	55	0.004475068	20.004728	1
GTTATAT	110	1.2599958E-7	20.004728	1
CGGTCCA	225	0.0	19.555447	10
TAGGACG	215	0.0	19.441753	4
CGCCGGT	230	0.0	19.13033	7
GTGTATA	255	0.0	18.98488	1
GCCGGTC	255	0.0	18.98029	8
GTAGAAC	360	0.0	18.94434	3
AACGCAG	1025	0.0	18.887703	6
CGTATAT	140	4.0708983E-9	18.857038	10
TAGACCA	140	4.0708983E-9	18.857038	4
>>END_MODULE