FastQCFastQC Report
Thu 2 Feb 2017
SRR4062630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703464
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT101420.595375071031733No Hit
GTCCTAAAGTGTGTATTTCTCATTT95540.5608571710350204No Hit
CTGTAGGACGTGGAATATGGCAAGA93390.5482358300498279No Hit
CTTTAGGACGTGAAATATGGCGAGG76760.4506112251271527No Hit
GTCCTACAGTGTGCATTTCTCATTT66940.3929639839761803No Hit
CTGTAGGACCTGGAATATGGCGAGA48280.283422485007021No Hit
CTGAAGGACCTGGAATATGGCGAGA38740.22741895337970158No Hit
GTCCTTCAGTGTGCATTTCTCATTT33550.19695162328056243No Hit
GATATACACTGTTCTACAAATCCCG33090.19425124334884683No Hit
TTGTAGAACAGTGTATATCAATGAG32740.1921966064442806No Hit
ATTTAGAAATGTCCACTGTAGGACG32230.1892027069547698No Hit
GAATATGGCAAGAAAACTGAAAATC28240.16577984624271486No Hit
GTGTATATCAATGAGTTACAATGAA26820.15744389080133186No Hit
GTATCAACGCAGAGTACTTTTTTTT25690.15081034879516092No Hit
TTTCTAAATTTTCCACCTTTTTCAG25550.14998849403333445No Hit
GTGTATATCAATGAGTTACAATGAG24660.1447638459045803No Hit
GGAATATGGCGAGAAAACTGAAAAT22300.13090972277664806No Hit
CTGTAGGACATGGAATATGGCAAGA21900.12856156631428664No Hit
GTTCTACAGTGTGGTTTTTATCATT21780.12785711937557823No Hit
ACCTGGAATATGGCGAGAAAACTGA20690.12145839301564343No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20600.12093005781161212No Hit
GACCTGGAATATGGCGAGAAAACTG20050.11770134267586518No Hit
CCATATTCCAGGTCCTTCAGTGTGC18340.10766297379927019No Hit
CCATATTTCACGTCCTAAAGTGTGT18240.10707593468367983No Hit
GCCATATTCCACGTCCTACAGTGGA18180.10672371121432563No Hit
GAAATATGGCGAGGAAAACTGAAAA17900.10508000169067266No Hit
GATATACACTGTTCTACAATGCCGG17680.1037885156363739No Hit
TTCCAGGTCCTTCAGTGTGCATTTC17660.10367110781325582No Hit
ACAGTGGACATTTCTAAATTTTCCA17080.10026628094283178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC250.00602950519.0015310
AGGCCCG653.3697615E-614.61656110
CAAGACG851.7183993E-814.5284454
GACGGAC801.2873898E-714.2511467
TTGCGAG1051.364242E-913.57611418
CCAACGA3300.013.534574519
AAGACGG852.697834E-713.412455
TAGGACC18000.013.0880174
ATATCGC751.4771074E-512.66805815
ACGGACC905.4006887E-712.66768558
AGGACGA1352.7284841E-1112.6673145
GTATCAA31000.012.52421
TCCAACG4950.012.479053518
CGGACCA1001.4412035E-712.3509949
GCGCAAG1001.4573016E-712.3404711
CCACCTT8150.012.2402513
GCCGCCT701.08750806E-412.21850118
GAAATGT11050.012.1231476
GGTATCA10500.012.1144561
ACCGGCA550.003066125612.09223716
Produced by FastQC (version 0.11.5)