##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062630_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1703464
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26990356121409	32.0	32.0	32.0	32.0	32.0
2	30.946729135455755	32.0	32.0	32.0	32.0	32.0
3	30.931539498339852	32.0	32.0	32.0	32.0	32.0
4	31.004055266210496	32.0	32.0	32.0	32.0	32.0
5	30.861883198001248	32.0	32.0	32.0	32.0	32.0
6	34.54364694528326	36.0	36.0	36.0	32.0	36.0
7	34.42432420057013	36.0	36.0	36.0	32.0	36.0
8	34.43408255178859	36.0	36.0	36.0	32.0	36.0
9	34.55742768852174	36.0	36.0	36.0	32.0	36.0
10	34.24211782579497	36.0	36.0	36.0	32.0	36.0
11	34.53925178342483	36.0	36.0	36.0	32.0	36.0
12	34.338818431149704	36.0	36.0	36.0	32.0	36.0
13	34.42679798340323	36.0	36.0	36.0	32.0	36.0
14	34.359442289358626	36.0	36.0	36.0	32.0	36.0
15	34.3044778169659	36.0	36.0	36.0	32.0	36.0
16	34.3313589251079	36.0	36.0	36.0	32.0	36.0
17	34.29823876524541	36.0	36.0	36.0	32.0	36.0
18	34.327795597676264	36.0	36.0	36.0	32.0	36.0
19	34.25217439288414	36.0	36.0	36.0	32.0	36.0
20	34.24325315944452	36.0	36.0	36.0	32.0	36.0
21	34.20209584705048	36.0	36.0	36.0	32.0	36.0
22	34.162346841494745	36.0	36.0	36.0	32.0	36.0
23	34.145622097091575	36.0	36.0	36.0	32.0	36.0
24	34.11240624985324	36.0	36.0	36.0	32.0	36.0
25	33.7067393264548	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	74.0
5	169.0
6	331.0
7	67.0
8	258.0
9	309.0
10	230.0
11	55.0
12	134.0
13	114.0
14	237.0
15	284.0
16	427.0
17	599.0
18	755.0
19	1044.0
20	1646.0
21	2450.0
22	3751.0
23	5708.0
24	8497.0
25	12190.0
26	17346.0
27	22726.0
28	31061.0
29	41557.0
30	54611.0
31	75306.0
32	108586.0
33	161475.0
34	374986.0
35	776478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28348402860989	20.545731970431984	12.204879039667663	24.965904961290462
2	11.893057119408434	21.690910136936765	46.710664583008004	19.705368160646795
3	18.818481328931117	27.30047951606919	29.96323788411773	23.91780127088196
4	10.474635979333021	17.62464772193518	40.928957256928136	30.971759041803665
5	10.588507510281602	40.94981263202527	36.871175047671514	11.590504810021617
6	28.586992974874352	40.211253004957705	19.66794373086386	11.533810289304084
7	25.919437727788626	32.71038798380483	23.472244741622195	17.897929546784347
8	22.33763257631401	42.54376120104592	21.070129564885274	14.048476657754797
9	26.74954238149664	15.1149555309403	21.510332041522084	36.62517004604098
10	13.87466944570328	31.076482872893752	36.15181016499648	18.897037516406485
11	33.33993615444253	22.281876196516414	27.58343213073821	16.794755518302843
12	23.79030338017256	25.15180178088223	34.67400167071609	16.383893168229115
13	29.581218303948237	23.978085199927584	26.74012352790428	19.700572968219895
14	18.46440247964101	25.408502398875154	28.81354138867891	27.313553732804923
15	19.953885659239354	37.90462454075206	23.36696988344462	18.77451991656396
16	18.11894118297025	29.612988011373854	33.628807496682235	18.63926330897366
17	18.261701184666695	31.93380350074475	30.828725904312037	18.975769410276516
18	18.12885264057619	29.056743083617363	35.945261494580585	16.86914278122586
19	22.818000117570982	27.10087590382694	28.463523602374934	21.61760037622715
20	22.953830889402095	29.60966655283141	30.3198339611906	17.11666859657589
21	21.10025083725089	28.1738965835696	27.452658657353496	23.273193921826017
22	21.682850750700428	32.54861839958371	28.318530996710265	17.449999853005593
23	19.101552303528134	31.834568325106588	30.218653510143707	18.84522586122157
24	22.207734412982123	28.315149434436552	31.634516859322524	17.8425992932588
25	20.042890348613412	30.573893557159504	31.13012776573969	18.2530883284874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	216.0
1	216.0
2	542.5
3	869.0
4	869.0
5	869.0
6	1774.0
7	2679.0
8	2679.0
9	2679.0
10	3816.5
11	4954.0
12	4954.0
13	4954.0
14	7538.0
15	10122.0
16	10122.0
17	10122.0
18	17320.0
19	24518.0
20	24518.0
21	24518.0
22	41495.0
23	58472.0
24	58472.0
25	58472.0
26	89248.0
27	120024.0
28	120024.0
29	120024.0
30	163064.5
31	206105.0
32	206105.0
33	206105.0
34	221432.0
35	236759.0
36	236759.0
37	236759.0
38	238680.0
39	240601.0
40	240601.0
41	240601.0
42	237024.0
43	233447.0
44	233447.0
45	233447.0
46	223974.0
47	214501.0
48	214501.0
49	214501.0
50	185959.5
51	157418.0
52	157418.0
53	157418.0
54	120683.5
55	83949.0
56	83949.0
57	83949.0
58	66666.5
59	49384.0
60	49384.0
61	49384.0
62	38848.5
63	28313.0
64	28313.0
65	28313.0
66	21820.0
67	15327.0
68	15327.0
69	15327.0
70	11264.5
71	7202.0
72	7202.0
73	7202.0
74	5580.0
75	3958.0
76	3958.0
77	3958.0
78	3175.0
79	2392.0
80	2392.0
81	2392.0
82	1641.0
83	890.0
84	890.0
85	890.0
86	683.5
87	477.0
88	477.0
89	477.0
90	328.0
91	179.0
92	179.0
93	179.0
94	123.5
95	68.0
96	68.0
97	68.0
98	354.0
99	640.0
100	640.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008042435883587796
2	0.00293519557795175
3	0.0046963129247228
4	0.01549783265158524
5	0.039038101186758274
6	0.050544067852329136
7	0.07208840339449497
8	0.09474811325628249
9	0.10091202396998117
10	0.12650692940972041
11	0.12744619199466498
12	0.1407132760070069
13	0.12609600202880716
14	0.13208380100782877
15	0.11981468349199044
16	0.11846449352613264
17	0.1306749071304119
18	0.13842382345620455
19	0.13877604692555873
20	0.12768100764090112
21	0.13859993519088165
22	0.15961593552901615
23	0.14722941019005978
24	0.1418873542381876
25	0.13983271733362138
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1703464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.950731774462305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.18347084120012	49.03726477628395
2	11.617506778756969	13.697210520049993
3	2.5651089301798127	4.536451455459242
4	0.8584364831515919	2.024218354547289
5	0.4089094858330408	1.2052756709688441
6	0.23999622107103358	0.8488771713145836
7	0.15084416033060605	0.6224661544775482
8	0.11696456549533041	0.5516117382105401
9	0.10023049514800639	0.5317794931582503
>10	0.621294255778102	7.19184724891694
>50	0.07233018293915126	2.9468959524380867
>100	0.053424267074276416	6.46552951673346
>500	0.0065904346154144165	2.688024805214251
>1k	0.004393623076942944	5.097499037148153
>5k	3.994202797220858E-4	1.9580362888893137
>10k+	9.985506993052146E-5	0.5970118161896227
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	10142	0.595375071031733	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	9554	0.5608571710350204	No Hit
CTGTAGGACGTGGAATATGGCAAGA	9339	0.5482358300498279	No Hit
CTTTAGGACGTGAAATATGGCGAGG	7676	0.4506112251271527	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6694	0.3929639839761803	No Hit
CTGTAGGACCTGGAATATGGCGAGA	4828	0.283422485007021	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3874	0.22741895337970158	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3355	0.19695162328056243	No Hit
GATATACACTGTTCTACAAATCCCG	3309	0.19425124334884683	No Hit
TTGTAGAACAGTGTATATCAATGAG	3274	0.1921966064442806	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3223	0.1892027069547698	No Hit
GAATATGGCAAGAAAACTGAAAATC	2824	0.16577984624271486	No Hit
GTGTATATCAATGAGTTACAATGAA	2682	0.15744389080133186	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2569	0.15081034879516092	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2555	0.14998849403333445	No Hit
GTGTATATCAATGAGTTACAATGAG	2466	0.1447638459045803	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2230	0.13090972277664806	No Hit
CTGTAGGACATGGAATATGGCAAGA	2190	0.12856156631428664	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2178	0.12785711937557823	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2069	0.12145839301564343	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2060	0.12093005781161212	No Hit
GACCTGGAATATGGCGAGAAAACTG	2005	0.11770134267586518	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1834	0.10766297379927019	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1824	0.10707593468367983	No Hit
GCCATATTCCACGTCCTACAGTGGA	1818	0.10672371121432563	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1790	0.10508000169067266	No Hit
GATATACACTGTTCTACAATGCCGG	1768	0.1037885156363739	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1766	0.10367110781325582	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1708	0.10026628094283178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	5.8703911559035E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	25	0.006029505	19.00153	10
AGGCCCG	65	3.3697615E-6	14.616561	10
CAAGACG	85	1.7183993E-8	14.528445	4
GACGGAC	80	1.2873898E-7	14.251146	7
TTGCGAG	105	1.364242E-9	13.576114	18
CCAACGA	330	0.0	13.5345745	19
AAGACGG	85	2.697834E-7	13.41245	5
TAGGACC	1800	0.0	13.088017	4
ATATCGC	75	1.4771074E-5	12.668058	15
ACGGACC	90	5.4006887E-7	12.6676855	8
AGGACGA	135	2.7284841E-11	12.667314	5
GTATCAA	3100	0.0	12.5242	1
TCCAACG	495	0.0	12.4790535	18
CGGACCA	100	1.4412035E-7	12.350994	9
GCGCAAG	100	1.4573016E-7	12.340471	1
CCACCTT	815	0.0	12.24025	13
GCCGCCT	70	1.08750806E-4	12.218501	18
GAAATGT	1105	0.0	12.123147	6
GGTATCA	1050	0.0	12.114456	1
ACCGGCA	55	0.0030661256	12.092237	16
>>END_MODULE