FastQCFastQC Report
Thu 2 Feb 2017
SRR4062630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703464
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA51680.3033818149370929No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC39920.23434601494366775No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA35550.20869240559236946No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA34170.2005912657972226No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA22050.12944212498767219No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG18140.10648889556808948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT300.00574594729.3330384
TAGGACC21650.027.2329834
GTATCAA34200.026.190251
AACCGCG651.8140965E-523.6920727
TGTAGGA62400.023.130012
CTGTAGG61050.022.8907171
GTAGGAC62150.022.5130483
AGGACCT39350.022.3631845
CGCGGTC703.2165986E-521.9997810
ACGGTAT1004.627327E-821.999789
CGAGCCG904.0842315E-721.99977915
GGTATCA13250.021.9244841
GGACCTG38600.021.885796
ACTGTTC17300.021.1096158
CTACGAC1158.778443E-921.0432684
ACGACGG1158.778443E-921.0432686
GTCCTAC54450.020.9768751
ACGGACC956.8703594E-720.8418968
CGGACCA956.8703594E-720.8418969
GGACGTC856.116381E-620.7056756
Produced by FastQC (version 0.11.5)