##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1703464
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35446654581488	32.0	32.0	32.0	32.0	32.0
2	31.519516702436917	32.0	32.0	32.0	32.0	32.0
3	31.585676597803065	32.0	32.0	32.0	32.0	32.0
4	31.664456660076173	32.0	32.0	32.0	32.0	32.0
5	31.6425354454218	32.0	32.0	32.0	32.0	32.0
6	35.25366488519863	36.0	36.0	36.0	36.0	36.0
7	35.269218486566196	36.0	36.0	36.0	36.0	36.0
8	35.21644073487904	36.0	36.0	36.0	36.0	36.0
9	35.334775492760635	36.0	36.0	36.0	36.0	36.0
10	35.25009451329761	36.0	36.0	36.0	36.0	36.0
11	35.331382406672525	36.0	36.0	36.0	36.0	36.0
12	35.28397488881479	36.0	36.0	36.0	36.0	36.0
13	35.298608599888226	36.0	36.0	36.0	36.0	36.0
14	35.27906782884757	36.0	36.0	36.0	36.0	36.0
15	35.24730196822475	36.0	36.0	36.0	36.0	36.0
16	35.27310351143317	36.0	36.0	36.0	36.0	36.0
17	35.27472667458778	36.0	36.0	36.0	36.0	36.0
18	35.275519177393825	36.0	36.0	36.0	36.0	36.0
19	35.247455185433914	36.0	36.0	36.0	36.0	36.0
20	35.23258020128397	36.0	36.0	36.0	36.0	36.0
21	35.21097363959555	36.0	36.0	36.0	36.0	36.0
22	35.19383268445943	36.0	36.0	36.0	36.0	36.0
23	35.17629195568559	36.0	36.0	36.0	36.0	36.0
24	35.15864614691006	36.0	36.0	36.0	36.0	36.0
25	35.12573379889449	36.0	36.0	36.0	36.0	36.0
26	35.10405444435574	36.0	36.0	36.0	36.0	36.0
27	35.07152484584353	36.0	36.0	36.0	36.0	36.0
28	35.03402713529608	36.0	36.0	36.0	36.0	36.0
29	35.00724817196019	36.0	36.0	36.0	36.0	36.0
30	34.96957082744338	36.0	36.0	36.0	36.0	36.0
31	34.963159186222896	36.0	36.0	36.0	36.0	36.0
32	34.93564407583606	36.0	36.0	36.0	36.0	36.0
33	34.957215415177544	36.0	36.0	36.0	36.0	36.0
34	34.93677706132915	36.0	36.0	36.0	36.0	36.0
35	34.91837279801628	36.0	36.0	36.0	36.0	36.0
36	34.869400233876384	36.0	36.0	36.0	36.0	36.0
37	34.81976725073145	36.0	36.0	36.0	36.0	36.0
38	34.78248909281323	36.0	36.0	36.0	36.0	36.0
39	34.668391583267976	36.0	36.0	36.0	32.0	36.0
40	34.72908027407682	36.0	36.0	36.0	36.0	36.0
41	34.696300596901374	36.0	36.0	36.0	32.0	36.0
42	34.70793982144618	36.0	36.0	36.0	36.0	36.0
43	34.68691853775601	36.0	36.0	36.0	32.0	36.0
44	34.62409126344907	36.0	36.0	36.0	32.0	36.0
45	34.611076606256425	36.0	36.0	36.0	32.0	36.0
46	34.50278784875994	36.0	36.0	36.0	32.0	36.0
47	34.51984779249811	36.0	36.0	36.0	32.0	36.0
48	34.46405735606975	36.0	36.0	36.0	32.0	36.0
49	34.41370172777353	36.0	36.0	36.0	32.0	36.0
50	33.985019935848364	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	8.0
21	28.0
22	92.0
23	312.0
24	801.0
25	1990.0
26	4339.0
27	8376.0
28	14557.0
29	23241.0
30	34203.0
31	49134.0
32	72153.0
33	118558.0
34	256378.0
35	1119290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80298407911826	21.156700556373636	12.695488183225182	25.344827181282923
2	11.622553782299466	22.178531696993357	46.18271158480564	20.016202935901536
3	18.357103651109067	27.579536882394894	30.42886369173239	23.634495774763643
4	10.321392552343404	18.13600773014015	41.117089785389986	30.42550993212646
5	10.341463242817719	41.209054731449996	36.78007681998376	11.669405205748525
6	28.825415536125842	40.273513585862204	19.35616996446061	11.544900913551343
7	25.85822284265807	32.78030270120495	23.5630146137689	17.79845984236807
8	22.356047651167064	42.66115554021426	20.935470861415833	14.047325947202847
9	26.533698393391347	15.040998811832832	21.5044168823057	36.92088591247012
10	13.892867709561225	31.236410044474084	35.90413416426763	18.966588081697058
11	33.33407691621308	22.2369829946509	27.711592378823386	16.717347710312634
12	23.849285925619796	25.180455824132476	34.588696914052775	16.381561336194952
13	29.604323895309793	23.89971258564901	26.845063940300466	19.65089957874073
14	18.581431717958232	25.457596990602678	28.66993373502463	27.29103755641446
15	20.039402065438424	37.89202472139124	23.385818543861213	18.682754669309126
16	18.272120807953677	29.723551539686195	33.31617222318757	18.688155429172557
17	18.26466541118568	32.08714713078762	30.70367204707584	18.944515410950864
18	18.35571517801374	29.01622810931138	35.789133201523484	16.838923511151396
19	22.942369195944266	26.926721081279087	28.647039209516606	21.48387051326004
20	23.1372074784087	29.65081739326455	30.219247368890684	16.992727759436065
21	21.168806619922698	28.069862351068174	27.602168287677344	23.15916274133178
22	21.829460440608077	32.35630456528579	28.472982111744074	17.341252882362056
23	19.222478432182893	31.806307617889196	30.13019353505563	18.841020414872283
24	22.28647039209517	28.377999182841553	31.468525310778507	17.867005114284776
25	20.25924821422701	30.41273546138926	30.95621627460281	18.371800049780916
26	17.071978040040765	32.05826480630057	32.255099021758014	18.614658131900644
27	18.573330578163084	31.166434981895712	30.950404587358467	19.309829852582737
28	17.740439480963495	32.19269676377076	28.86242386102671	21.204439894239034
29	19.937550778883498	29.133753340252568	28.47668045817229	22.452015422691645
30	18.197332024627467	30.109118830805937	30.55286169828068	21.140687446285924
31	21.853529044347283	28.302505952576634	29.759302221825646	20.08466278125044
32	19.192598141199344	31.261300502975114	30.15690381481499	19.389197541010553
33	18.223572673094353	31.386222426772743	32.154010886053356	18.236194014079548
34	19.334426791525974	31.247916011139655	31.852037964993684	17.56561923234069
35	18.370215044168823	31.852625004109274	31.3827001920792	18.394459759642704
36	17.44991382265783	30.92328338021819	32.66649603396374	18.960306763160244
37	18.28163084162624	30.198113960729433	29.238715934120123	22.28153926352421
38	19.303842053603717	32.41999830932735	28.926235012891375	19.349924624177557
39	21.458040792174064	28.296752969243848	30.212907346442307	20.03229889213978
40	22.895053843227682	28.310254868902422	30.818320786350633	17.976370501519256
41	21.782673423095527	27.008378222257708	32.56235529485801	18.64659305978876
42	19.84926009589871	31.38504834854156	31.194201932063137	17.571489623496593
43	17.759987883512654	31.418039946837737	32.141037321598816	18.680934848050796
44	17.236114176759827	31.297051185114565	30.991145101980432	20.475689536145172
45	17.711087525183977	31.5457796583902	30.43392757346208	20.30920524296375
46	18.51853373807795	28.215028110417556	31.130504308581173	22.13593384292332
47	21.381503443279954	28.482567569709467	30.364909598858326	19.771019388152254
48	17.936040914278202	29.480693457566463	30.259400844397067	22.32386478375827
49	20.470476904987077	28.674529473196657	30.13936903824738	20.715624583568886
50	18.199525437021784	32.074563447849144	28.95867357182021	20.767237543308863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	21.5
2	27.0
3	69.5
4	112.0
5	156.5
6	201.0
7	206.0
8	211.0
9	319.5
10	428.0
11	701.5
12	975.0
13	1592.5
14	2210.0
15	3155.5
16	4101.0
17	4779.0
18	5457.0
19	6469.0
20	7481.0
21	9223.0
22	10965.0
23	14003.0
24	17041.0
25	22030.0
26	27019.0
27	38246.0
28	49473.0
29	61951.0
30	74429.0
31	90021.5
32	105614.0
33	129071.5
34	152529.0
35	176408.0
36	200287.0
37	199064.5
38	197842.0
39	181497.0
40	165152.0
41	149121.5
42	133091.0
43	120089.5
44	107088.0
45	98973.0
46	90858.0
47	84954.5
48	79051.0
49	72997.0
50	66943.0
51	59413.5
52	51884.0
53	45976.5
54	40069.0
55	35815.0
56	31561.0
57	27573.5
58	23586.0
59	20496.5
60	17407.0
61	14814.5
62	12222.0
63	10333.0
64	8444.0
65	7216.5
66	5989.0
67	5221.5
68	4454.0
69	3968.5
70	3483.0
71	2731.0
72	1979.0
73	1731.5
74	1484.0
75	1080.5
76	677.0
77	649.0
78	621.0
79	502.0
80	383.0
81	315.5
82	248.0
83	210.0
84	172.0
85	145.5
86	119.0
87	86.0
88	53.0
89	37.0
90	21.0
91	12.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017141542175238222
2	0.004050569897573415
3	5.283352040313151E-4
4	1.1740782311807001E-4
5	5.8703911559035005E-5
6	1.1740782311807001E-4
7	1.1740782311807001E-4
8	5.8703911559035005E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.76111734677105E-4
47	5.8703911559035005E-5
48	0.0
49	5.8703911559035005E-5
50	1.1740782311807001E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1703464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.8760728660985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.24862176898803	58.869628335981794
2	11.207540559897138	15.662778416262535
3	2.402483607854564	5.036283589261583
4	0.7687586162347628	2.1487133233784537
5	0.3723596842198204	1.3009516213470798
6	0.20378059170464488	0.8543632484790261
7	0.12835926687333293	0.6278469039555996
8	0.09481802487260323	0.5300408972014049
9	0.07085205695589304	0.4455777145106649
>10	0.431766069937528	5.750641412269411
>50	0.040798503091297005	1.9782926947831336
>100	0.02588586499372208	3.433396922016204
>500	0.002452896743094366	1.2135034441986214
>1k	0.0014379049873311802	1.8425370091998843
>5k	8.458264631359883E-5	0.3054444671546111
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5168	0.3033818149370929	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	3992	0.23434601494366775	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	3555	0.20869240559236946	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	3417	0.2005912657972226	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2205	0.12944212498767219	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1814	0.10648889556808948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.8703911559035E-5	0.0	0.0	0.0
6	0.0	5.8703911559035E-5	0.0	0.0	0.0
7	0.0	5.8703911559035E-5	0.0	0.0	0.0
8	0.0	5.8703911559035E-5	0.0	0.0	0.0
9	0.0	5.8703911559035E-5	0.0	0.0	0.0
10	0.0	5.8703911559035E-5	0.0	1.76111734677105E-4	0.0
11	0.0	5.8703911559035E-5	0.0	1.76111734677105E-4	0.0
12	0.0	5.8703911559035E-5	0.0	1.76111734677105E-4	0.0
13	0.0	5.8703911559035E-5	0.0	2.3481564623614E-4	0.0
14	0.0	5.8703911559035E-5	0.0	2.3481564623614E-4	0.0
15	0.0	5.8703911559035E-5	0.0	2.3481564623614E-4	0.0
16	0.0	5.8703911559035E-5	0.0	2.3481564623614E-4	0.0
17	0.0	5.8703911559035E-5	0.0	3.5222346935421E-4	0.0
18	0.0	5.8703911559035E-5	0.0	4.6963129247228E-4	0.0
19	0.0	5.8703911559035E-5	0.0	5.8703911559035E-4	0.0
20	0.0	5.8703911559035E-5	0.0	0.001115374319621665	0.0
21	0.0	5.8703911559035E-5	0.0	0.0011740782311807	0.0
22	0.0	5.8703911559035E-5	0.0	0.001585005612093945	0.0
23	0.0	1.1740782311807E-4	0.0	0.001937229081448155	0.0
24	0.0	1.1740782311807E-4	0.0	0.00317001122418789	0.0
25	0.0	1.1740782311807E-4	0.0	0.003580938605101135	0.0
26	0.0	1.1740782311807E-4	0.0	0.005107240305636045	0.0
27	0.0	1.1740782311807E-4	0.0	0.0058703911559035	0.0
28	0.0	1.1740782311807E-4	0.0	0.0105667040806263	0.0
29	0.0	1.1740782311807E-4	0.0	0.017552469556151466	0.0
30	0.0	1.1740782311807E-4	0.0	0.0353984586700981	0.0
31	0.0	2.3481564623614E-4	0.0	0.08576641478775014	0.0
32	0.0	2.3481564623614E-4	0.0	0.15744389080133186	0.0
33	0.0	2.3481564623614E-4	0.0	0.2369876909638243	0.0
34	0.0	2.3481564623614E-4	0.0	0.3319706198663429	0.0
35	0.0	2.3481564623614E-4	0.0	0.43059319128552176	0.0
36	0.0	2.3481564623614E-4	0.0	0.5706020203538202	0.0
37	0.0	2.3481564623614E-4	0.0	0.783227588020645	0.0
38	0.0	2.3481564623614E-4	0.0	1.059781715375259	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGGT	30	0.005745947	29.333038	4
TAGGACC	2165	0.0	27.232983	4
GTATCAA	3420	0.0	26.19025	1
AACCGCG	65	1.8140965E-5	23.692072	7
TGTAGGA	6240	0.0	23.13001	2
CTGTAGG	6105	0.0	22.890717	1
GTAGGAC	6215	0.0	22.513048	3
AGGACCT	3935	0.0	22.363184	5
CGCGGTC	70	3.2165986E-5	21.99978	10
ACGGTAT	100	4.627327E-8	21.99978	9
CGAGCCG	90	4.0842315E-7	21.999779	15
GGTATCA	1325	0.0	21.924484	1
GGACCTG	3860	0.0	21.88579	6
ACTGTTC	1730	0.0	21.109615	8
CTACGAC	115	8.778443E-9	21.043268	4
ACGACGG	115	8.778443E-9	21.043268	6
GTCCTAC	5445	0.0	20.976875	1
ACGGACC	95	6.8703594E-7	20.841896	8
CGGACCA	95	6.8703594E-7	20.841896	9
GGACGTC	85	6.116381E-6	20.705675	6
>>END_MODULE