FastQCFastQC Report
Thu 2 Feb 2017
SRR4062629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4631
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT200.4318721658389117No Hit
GTCCTACAGTGGACATTTCTAAATT160.3454977326711294No Hit
GTCCTACAGTGTGCATTTCTCATTT160.3454977326711294No Hit
CTTTAGGACGTGAAATATGGCGAGG150.32390412437918376No Hit
CTGTAGGACGTGGAATATGGCAAGA140.3023105160872382No Hit
GTCCTAAAGTGTGTATTTCTCATTT130.28071690779529257No Hit
CTCCACTCCTGGTGGTGCCCTTCCG110.23752969121140144No Hit
CTGTAGGACCTGGAATATGGCGAGA100.21593608291945585No Hit
TATCAACGCAGAGTACTTTTTTTTT100.21593608291945585No Hit
TTGTAGAACAGTGTATATCAATGAG100.21593608291945585No Hit
GAATAGGACCGCGGTTCTATTTTGT90.19434247462751025No Hit
ATTTAGAAATGTCCACTGTAGGACG80.1727488663355647No Hit
GATATACACTGTTCTACAATGCCGG80.1727488663355647No Hit
GAATATGGCAAGAAAACTGAAAATC80.1727488663355647No Hit
CTGAAGGACCTGGAATATGGCGAGA70.1511552580436191No Hit
GTCTTGCGCCGGTCCAAGAATTTCA70.1511552580436191No Hit
GTGTATATCAATGAGTTACAATGAA70.1511552580436191No Hit
GAAATACACACTTTAGGACGTGAAA70.1511552580436191No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG70.1511552580436191No Hit
ACCATACTCCCCCCGGAACCCAAAG70.1511552580436191No Hit
GCCATGCACCACCACCCACGGAATC70.1511552580436191No Hit
GGCTCCACTCCTGGTGGTGCCCTTC70.1511552580436191No Hit
CCCTTAGAGCCAATCCTTTTCCCGA70.1511552580436191No Hit
GGACTCTTTCGAGGCCCTGTAATTG60.1295616497516735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG60.1295616497516735No Hit
GCGCAAGACGGACCAGAGCGAAAGC60.1295616497516735No Hit
GTCAATCCTGTCCGTGTCCGGGCCG60.1295616497516735No Hit
TATTCCAGGTCCTTCAGTGTGCATT60.1295616497516735No Hit
GTGCATGGCCGTTCTTAGTTGGTGG60.1295616497516735No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT60.1295616497516735No Hit
GTATCTGATCGTCTTCGAACCTCCG50.10796804145972792No Hit
AAATTAAGCCGCAGGCTCCACTCCT50.10796804145972792No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50.10796804145972792No Hit
AGTGTGTATTTCTCATTTTCCGTGA50.10796804145972792No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50.10796804145972792No Hit
CTGTAGGACATGGAATATGGCAAGA50.10796804145972792No Hit
GGTATCAACGCAGAGTACTTTTTTT50.10796804145972792No Hit
ACAGTGGACATTTCTAAATTTTCCA50.10796804145972792No Hit
CTCCGAGGTCACCCCAACCGAAATT50.10796804145972792No Hit
ACCTGGAATATGGCGAGAAAACTGA50.10796804145972792No Hit
CTGTAGAACATATTAGATGAGTGAG50.10796804145972792No Hit
CTCCAATGGATCCTCGTTAAAGGAT50.10796804145972792No Hit
GAACTACGACGGTATCTGATCGTCT50.10796804145972792No Hit
GTTATTGCTCAATCTCGGGTGGCTG50.10796804145972792No Hit
ATATTAAAGTTGCTGCAGTTAAAAA50.10796804145972792No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG50.10796804145972792No Hit
CTCGGGGGTCGCGTAACTAGTTAGC50.10796804145972792No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)