FastQCFastQC Report
Thu 2 Feb 2017
SRR4062629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4631
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.28071690779529257No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG100.21593608291945585No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC90.19434247462751025No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA90.19434247462751025No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT90.19434247462751025No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC90.19434247462751025No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT80.1727488663355647No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG70.1511552580436191No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC60.1295616497516735No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA60.1295616497516735No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA60.1295616497516735No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC60.1295616497516735No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC60.1295616497516735No Hit
CCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTT50.10796804145972792No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG50.10796804145972792No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG50.10796804145972792No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT50.10796804145972792No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC50.10796804145972792No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTG50.10796804145972792No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA50.10796804145972792No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC50.10796804145972792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC207.066209E-444.03
GAAAATG301.1106777E-436.66666841
AAAATGA301.1106777E-436.66666842
AAATGAG301.1106777E-436.66666843
AATGAGA301.1106777E-436.66666844
GAAAATC352.7502302E-431.42857231
GAATATG300.005177378729.33333613
ACTGAAA406.017586E-427.528
AAAATCA406.017586E-427.532
AATATGG406.017586E-427.514
TGAAAAT406.017586E-427.530
GGAAAAT406.017586E-427.540
CTGAAAA506.706627E-526.40000229
Produced by FastQC (version 0.11.5)