FastQCFastQC Report
Thu 2 Feb 2017
SRR4062628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543536
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG76840.2168455463695021No Hit
TCCATGTACTCTGCGTTGATACCAC64700.18258598191185302No Hit
GTATCAACGCAGAGTACTTTTTTTT53820.15188218773564033No Hit
GTCTTGCGCCGGTCCAAGAATTTCA38800.10949514834899378No Hit
GCGCAAGACGGACCAGAGCGAAAGC37630.10619336165908855No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG37590.10608048006285246No Hit
GAATAGGACCGCGGTTCTATTTTGT35710.1007750450397569No Hit
TATCAACGCAGAGTACTTTTTTTTT35530.10026707785669454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA8050.012.62835410
CAAGACG8050.012.0351814
CGCAAGA7150.011.8199532
AAGACGG8050.011.7996985
GGTCCAA9500.011.60028611
ATCGCCA5300.011.47408816
TATACCG1001.9347262E-611.3985075
ACGCGCG2350.011.32022713
TTAGACT1850.011.295344
AACCGCG2300.011.1529217
CGACCAT6000.011.08423110
GCAAGAC8950.011.0351663
TATAAGG2250.010.9729312
GCGCGCT2600.010.96273715
CGCGTAT1303.2676326E-810.9622727
AGAACCG3300.010.9379625
TCGCGTA3050.010.9025249
GTATTAG5800.010.8040451
GTTCAAA8700.010.8040451
CGCATCG5300.010.75574113
Produced by FastQC (version 0.11.5)