FastQCFastQC Report
Thu 2 Feb 2017
SRR4062628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543536
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC39890.11257117184642684No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT38000.10723751642427225No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT37280.10520564769202288No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA35590.10043640025104866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG801.3101089E-724.752351
CGTACGA450.001398317424.445732
GCGTATT808.995081E-519.2499269
GGCGTTA9400.017.31908242
TTAACGG3700.017.24317735
TAACGGC4200.016.7618436
CGCAATA11300.016.54860936
CTAGCGG11650.016.42912329
TCTAGCG11950.016.01667628
TAACGCA1105.6263467E-515.9999394
ACCGTCG8150.015.6563818
GTATTAT3400.015.5308861
ATACGAA12100.015.45448740
GTACTAA2303.6379788E-1115.30581
GATATAC7950.015.221571
TACCGTC8400.015.1904177
CGACCAT12150.015.02874810
TAGCGGC12750.015.01170630
CGGCGTT11150.014.99545941
GTACCGT2509.094947E-1214.9599426
Produced by FastQC (version 0.11.5)