FastQCFastQC Report
Thu 2 Feb 2017
SRR4062627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729361
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28820.39514040372325915No Hit
GTACATGGAAGCAGTGGTATCAACG23630.3239822255371483No Hit
TCCATGTACTCTGCGTTGATACCAC22380.30684393599328724No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20520.2813421611520221No Hit
TATCAACGCAGAGTACTTTTTTTTT19590.2685912737313895No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18310.2510416652384759No Hit
GTATTAGAGGCACTGCCTGCCCAGT16190.22197512617208762No Hit
GTCCTAAAGTGTGTATTTCTCATTT15040.20620789979173554No Hit
CCATAGGGTCTTCTCGTCTTATTAT14670.20113496608675263No Hit
CTCTAATACTTGTAATGCTAGAGGT14230.19510228816731356No Hit
GTCCTACAGTGGACATTTCTAAATT13660.18728722813531296No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13330.18276271969573366No Hit
CCATTGGGATGTCCTGATCCAACAT12820.17577029756183837No Hit
GTCCTACAGTGTGCATTTCTCATTT12720.1743992343983295No Hit
CTTTAGGACGTGAAATATGGCGAGG12350.16932630069334664No Hit
CTGTAGGACGTGGAATATGGCAAGA12040.16507600488646912No Hit
GTGTAAAGGTATAAGGGAGCTTGAC11290.15479303116015253No Hit
GATATATTTTGATCAACGGACCAAG10760.14752639639355544No Hit
CTTCTACACCATTGGGATGTCCTGA10610.14546980164829212No Hit
GAGTACATGGAAGCAGTGGTATCAA10590.14519558901559038No Hit
CCTTTACACTCTGCGAATGATTTCC10520.14423584480113416No Hit
CCCTTATACCTTTACACTCTGCGAA10080.13820316688169507No Hit
CATGTACTCTGCGTTGATACCACTG9770.13395287107481754No Hit
GTCAGGATACCGCGGCCGTTAAACT9760.13381576475846665No Hit
GGTATCAACGCAGAGTACTTTTTTT9640.132170488962256No Hit
ATCTATAACTTTATAGATGCAACAC9570.13121074474779978No Hit
GTATCCTGACCGTGCAAAGGTAGCA9550.130936532115098No Hit
ATCGTAAATAGATAGAAACCGACCT8990.12325857839944829No Hit
CTGTTAGTATGAGTAACAAGAATTC8970.12298436576674651No Hit
ATCCTGACCGTGCAAAGGTAGCATA8870.12161330260323762No Hit
GCCTAAAGGAAAGATCCAAAAAGAT8790.12051645207243052No Hit
GTTATAGATTAACCCAATTTTAAGT8780.12037934575607964No Hit
GTTATATAATTTAAGCTCCATAGGG8720.1195567078579743No Hit
CCTCTAGCATTACAAGTATTAGAGG8700.11928249522527254No Hit
CTGTAGGACCTGGAATATGGCGAGA8000.10968505308071037No Hit
TCCCAATGGTGTAGAAGCTATTAAT7750.10625739517193818No Hit
GATTAAACCTTGTACCTTTTGCATA7740.10612028885558727No Hit
GTACATGGGAGAAATCGTAAATAGA7690.10543475727383285No Hit
AAATATATCTGGGTCAATAAGATAT7590.10406369411032398No Hit
GTATAAGGGAGCTTGACTGCGAGAG7580.10392658779397308No Hit
CACTATAAATAATCCACCTATAACT7470.10241841831411332No Hit
GTCCTAAGGTAGCGAAATTCCTTGT7450.10214420568141153No Hit
CTATAGAACTAGTACCGCAAGGGAA7430.10186999304870976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC250.006029321318.999864
AGGCGTC350.002167820716.28671615
ATGTACG1200.015.83430717
ACCGGGC551.1245458E-515.5485448
TTATACC1550.015.322474
GGATAAG508.76572E-515.1873771
CACCGGG456.74605E-414.7807157
GTCCTAT653.395333E-614.6032471
GTAAAGG2300.014.4524483
TGTAAAG2300.014.4484812
TAGGACC4700.014.3509614
CGGCACG400.00526858614.2528329
CAGGCGT400.005273388714.25087514
CCACCTT1800.014.25087513
GTACGAA1350.014.07493919
AGGTATA2300.014.046277
TAGGCCC759.653195E-713.9332334
ATAGGAC759.683135E-713.9294073
TGCGAAT1600.013.65896512
AGCCCGA707.2350012E-613.5741266
Produced by FastQC (version 0.11.5)