##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062627_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729361
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.064220598578757	32.0	32.0	32.0	32.0	32.0
2	30.825236062800176	32.0	32.0	32.0	32.0	32.0
3	30.83471696457584	32.0	32.0	32.0	32.0	32.0
4	30.76000636173308	32.0	32.0	32.0	32.0	32.0
5	30.803278486236582	32.0	32.0	32.0	32.0	32.0
6	34.241072664976606	36.0	36.0	36.0	32.0	36.0
7	34.14541358805859	36.0	36.0	36.0	32.0	36.0
8	34.04437720141329	36.0	36.0	36.0	32.0	36.0
9	34.22404543154899	36.0	36.0	36.0	32.0	36.0
10	33.96242327187771	36.0	36.0	36.0	32.0	36.0
11	34.2705409255499	36.0	36.0	36.0	32.0	36.0
12	34.092552247789506	36.0	36.0	36.0	32.0	36.0
13	34.197123235270325	36.0	36.0	36.0	32.0	36.0
14	34.0668379581579	36.0	36.0	36.0	32.0	36.0
15	34.057727243436375	36.0	36.0	36.0	32.0	36.0
16	34.11656916122469	36.0	36.0	36.0	32.0	36.0
17	34.023480827738254	36.0	36.0	36.0	32.0	36.0
18	34.12479279807941	36.0	36.0	36.0	32.0	36.0
19	34.05882409396718	36.0	36.0	36.0	32.0	36.0
20	33.95441900512915	36.0	36.0	36.0	32.0	36.0
21	33.92570757142211	36.0	36.0	36.0	32.0	36.0
22	33.90138079771197	36.0	36.0	36.0	32.0	36.0
23	33.877237746465745	36.0	36.0	36.0	32.0	36.0
24	33.83325404018038	36.0	36.0	36.0	32.0	36.0
25	33.250885364037835	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	30.0
5	68.0
6	111.0
7	32.0
8	112.0
9	145.0
10	92.0
11	26.0
12	59.0
13	45.0
14	249.0
15	468.0
16	638.0
17	943.0
18	1136.0
19	1466.0
20	1724.0
21	2018.0
22	2364.0
23	3088.0
24	3829.0
25	5032.0
26	6938.0
27	9197.0
28	12665.0
29	17749.0
30	24461.0
31	35906.0
32	55904.0
33	86197.0
34	194297.0
35	262367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73397961072528	21.35361748513996	11.774360521318242	25.138042382816515
2	14.394443172360653	21.356108355945196	41.40623607468152	22.84321239701263
3	18.142254824666644	26.491344736571488	31.047338292256537	24.31906214650533
4	10.481070911404625	17.508721312914126	41.767135460090394	30.243072315590858
5	10.946080878681977	39.9828828186292	36.73665326186544	12.334383040823381
6	27.85761119752873	41.6177652677986	18.722994493963114	11.801629040709557
7	25.658186288431644	34.33668240359247	22.81758200145707	17.187549306518818
8	24.785821617504787	36.964519736796966	21.045950748921047	17.203707896777203
9	26.673803102977406	15.157379006525723	21.891430100664923	36.27738778983195
10	14.886166306324858	27.954837159820173	35.086584989189745	22.072411544665226
11	32.528294634829344	23.54182252451973	26.683367765049866	17.246515075601067
12	22.283042207422234	27.527564633247252	31.843998797329416	18.3453943620011
13	26.75119803098751	22.73039875797372	28.486850142830278	22.031553068208492
14	20.274599919283766	24.36888965761492	27.644074357770588	27.71243606533073
15	23.119537809647085	31.040271450356947	25.367211553111673	20.47297918688429
16	22.076481730584458	29.155845938452245	27.767452713646474	21.000219617316827
17	19.633510368864442	29.165700040769877	29.338114070549736	21.862675519815944
18	19.85563036100647	27.92473448946195	32.42097835279129	19.79865679674029
19	23.264648261868185	27.569031723085075	28.409644740436878	20.75667527460986
20	21.436553129101164	28.506476747953197	30.213109443038118	19.84386067990753
21	23.04955045860224	26.187897184136933	27.66289268803653	23.099659669224295
22	21.26606368765559	29.712621503200037	27.9570985056428	21.06421630350157
23	20.988701844857342	29.409527575379386	29.531721743285154	20.070048836478115
24	21.70966736637475	29.3641259804036	28.388012613965696	20.53819403925596
25	20.971929154991646	28.917212492466437	29.589226901992976	20.521631450548938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	141.0
2	464.5
3	788.0
4	788.0
5	788.0
6	2493.0
7	4198.0
8	4198.0
9	4198.0
10	4435.0
11	4672.0
12	4672.0
13	4672.0
14	4853.0
15	5034.0
16	5034.0
17	5034.0
18	8281.0
19	11528.0
20	11528.0
21	11528.0
22	18145.0
23	24762.0
24	24762.0
25	24762.0
26	36083.0
27	47404.0
28	47404.0
29	47404.0
30	57040.0
31	66676.0
32	66676.0
33	66676.0
34	72524.0
35	78372.0
36	78372.0
37	78372.0
38	78508.5
39	78645.0
40	78645.0
41	78645.0
42	82726.5
43	86808.0
44	86808.0
45	86808.0
46	94149.5
47	101491.0
48	101491.0
49	101491.0
50	92621.0
51	83751.0
52	83751.0
53	83751.0
54	69229.5
55	54708.0
56	54708.0
57	54708.0
58	44654.5
59	34601.0
60	34601.0
61	34601.0
62	28039.0
63	21477.0
64	21477.0
65	21477.0
66	17140.5
67	12804.0
68	12804.0
69	12804.0
70	9452.0
71	6100.0
72	6100.0
73	6100.0
74	4502.0
75	2904.0
76	2904.0
77	2904.0
78	2149.5
79	1395.0
80	1395.0
81	1395.0
82	967.0
83	539.0
84	539.0
85	539.0
86	369.5
87	200.0
88	200.0
89	200.0
90	133.5
91	67.0
92	67.0
93	67.0
94	44.0
95	21.0
96	21.0
97	21.0
98	148.0
99	275.0
100	275.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0076779537156497265
2	0.0032905515924213113
3	0.004935827388631967
4	0.01549301374765034
5	0.03688159909838886
6	0.04702746650835457
7	0.06882737080814576
8	0.08857068036267363
9	0.0967970593437269
10	0.12147619628688675
11	0.11873406995986897
12	0.13463840265657198
13	0.11983092049067609
14	0.12106487733783407
15	0.11379824257123701
16	0.112564285724079
17	0.1209277710214832
18	0.13066231948239623
19	0.132170488962256
20	0.12462964156295717
21	0.13052521316604535
22	0.14917167218976612
23	0.13875159214709862
24	0.13189627632955422
25	0.13079942579874712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	729361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.57500700348083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7800842799069	24.7716684641528
2	14.215289642525475	9.545568986086025
3	4.763369559081628	4.797904989189991
4	2.057067074168526	2.7626416568735235
5	1.0423138660411053	1.7497847676077642
6	0.6630814997468917	1.3357779598728277
7	0.4427906137591255	1.040668857062675
8	0.33442979445436505	0.8982786152782369
9	0.25239572125972337	0.7626769298049427
>10	1.7895075950959696	12.246770700487653
>50	0.2976910908812215	7.083787733477783
>100	0.3230787766622222	21.69488186075751
>500	0.029891952720332345	6.715609497103453
>1k	0.009008533696538515	4.593978982244803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2882	0.39514040372325915	No Hit
GTACATGGAAGCAGTGGTATCAACG	2363	0.3239822255371483	No Hit
TCCATGTACTCTGCGTTGATACCAC	2238	0.30684393599328724	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2052	0.2813421611520221	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1959	0.2685912737313895	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1831	0.2510416652384759	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1619	0.22197512617208762	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1504	0.20620789979173554	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1467	0.20113496608675263	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1423	0.19510228816731356	No Hit
GTCCTACAGTGGACATTTCTAAATT	1366	0.18728722813531296	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1333	0.18276271969573366	No Hit
CCATTGGGATGTCCTGATCCAACAT	1282	0.17577029756183837	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1272	0.1743992343983295	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1235	0.16932630069334664	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1204	0.16507600488646912	No Hit
GTGTAAAGGTATAAGGGAGCTTGAC	1129	0.15479303116015253	No Hit
GATATATTTTGATCAACGGACCAAG	1076	0.14752639639355544	No Hit
CTTCTACACCATTGGGATGTCCTGA	1061	0.14546980164829212	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1059	0.14519558901559038	No Hit
CCTTTACACTCTGCGAATGATTTCC	1052	0.14423584480113416	No Hit
CCCTTATACCTTTACACTCTGCGAA	1008	0.13820316688169507	No Hit
CATGTACTCTGCGTTGATACCACTG	977	0.13395287107481754	No Hit
GTCAGGATACCGCGGCCGTTAAACT	976	0.13381576475846665	No Hit
GGTATCAACGCAGAGTACTTTTTTT	964	0.132170488962256	No Hit
ATCTATAACTTTATAGATGCAACAC	957	0.13121074474779978	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	955	0.130936532115098	No Hit
ATCGTAAATAGATAGAAACCGACCT	899	0.12325857839944829	No Hit
CTGTTAGTATGAGTAACAAGAATTC	897	0.12298436576674651	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	887	0.12161330260323762	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	879	0.12051645207243052	No Hit
GTTATAGATTAACCCAATTTTAAGT	878	0.12037934575607964	No Hit
GTTATATAATTTAAGCTCCATAGGG	872	0.1195567078579743	No Hit
CCTCTAGCATTACAAGTATTAGAGG	870	0.11928249522527254	No Hit
CTGTAGGACCTGGAATATGGCGAGA	800	0.10968505308071037	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	775	0.10625739517193818	No Hit
GATTAAACCTTGTACCTTTTGCATA	774	0.10612028885558727	No Hit
GTACATGGGAGAAATCGTAAATAGA	769	0.10543475727383285	No Hit
AAATATATCTGGGTCAATAAGATAT	759	0.10406369411032398	No Hit
GTATAAGGGAGCTTGACTGCGAGAG	758	0.10392658779397308	No Hit
CACTATAAATAATCCACCTATAACT	747	0.10241841831411332	No Hit
GTCCTAAGGTAGCGAAATTCCTTGT	745	0.10214420568141153	No Hit
CTATAGAACTAGTACCGCAAGGGAA	743	0.10186999304870976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.113189490526639E-4	0.0
10	0.0	0.0	0.0	4.113189490526639E-4	0.0
11	0.0	0.0	0.0	5.484252654035518E-4	0.0
12	0.0	0.0	0.0	5.484252654035518E-4	0.0
13	0.0	0.0	0.0	5.484252654035518E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCCC	25	0.0060293213	18.99986	4
AGGCGTC	35	0.0021678207	16.286716	15
ATGTACG	120	0.0	15.834307	17
ACCGGGC	55	1.1245458E-5	15.548544	8
TTATACC	155	0.0	15.32247	4
GGATAAG	50	8.76572E-5	15.187377	1
CACCGGG	45	6.74605E-4	14.780715	7
GTCCTAT	65	3.395333E-6	14.603247	1
GTAAAGG	230	0.0	14.452448	3
TGTAAAG	230	0.0	14.448481	2
TAGGACC	470	0.0	14.350961	4
CGGCACG	40	0.005268586	14.252832	9
CAGGCGT	40	0.0052733887	14.250875	14
CCACCTT	180	0.0	14.250875	13
GTACGAA	135	0.0	14.074939	19
AGGTATA	230	0.0	14.04627	7
TAGGCCC	75	9.653195E-7	13.933233	4
ATAGGAC	75	9.683135E-7	13.929407	3
TGCGAAT	160	0.0	13.658965	12
AGCCCGA	70	7.2350012E-6	13.574126	6
>>END_MODULE