FastQCFastQC Report
Thu 2 Feb 2017
SRR4062627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729361
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT16300.22348329565194738No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15410.21128083349671836No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG14120.1935941186874538No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13980.19167463025854137No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT12880.1765929354599437No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA11150.15287354273124007No Hit
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG10740.1472521837608537No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10390.1424534626885726No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC10320.14149371847411638No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC9940.13628367845278264No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC9810.1345012963402211No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC9530.13066231948239623No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA9510.13038810684969446No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9430.12929125631888735No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC8930.12243594050134296No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC8840.12120198365418497No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA8390.11503219941839501No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA8360.11462088046934234No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC8280.11352402993853525No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT8100.11105611624421927No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT8090.11091900992786838No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG7870.10790267096814883No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT7750.10625739517193818No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT7730.1059831825392364No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT7620.10447501305937663No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT7430.10186999304870976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT951.8189894E-1230.1112211
ATATGCG407.02976E-427.4997843
CCTTATA2500.024.6431882
TGTTACG901.5106707E-824.44425416
CCCTTAT2900.024.2806681
TAGGACC5700.023.1577154
GGACCGT1550.022.7095016
GCGCAAG500.002576502722.0043541
GTACTAT703.2081945E-522.0043531
TCCGAAA500.002579582221.99982812
ACTATAC703.2132928E-521.9998263
AGGTATA2350.021.5317487
CGCGGTA4400.021.4998343
GATATAC2050.021.467661
ATAGGAC956.859136E-720.8419423
TTAGCGT1800.020.77761715
GCACGAA1800.020.77761743
AGTGCCC1800.020.77761710
TTCGTCA4450.020.76388444
CCGCGGT4450.020.76388442
Produced by FastQC (version 0.11.5)