FastQCFastQC Report
Thu 2 Feb 2017
SRR4062626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059528
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30920.29182805928677674No Hit
TATCAACGCAGAGTACTTTTTTTTT19130.18055209489508536No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18590.17545548583897735No Hit
GCGCAAGACGGACCAGAGCGAAAGC15810.14921738736493986No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14970.1412893288332163No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13910.13128487401937466No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13880.13100172907181312No Hit
GAACTACGACGGTATCTGATCGTCT13570.12807589794701035No Hit
GAATAGGACCGCGGTTCTATTTTGT13380.12628264661245384No Hit
GAATAACGCCGCCGCATCGCCAGTC12530.11826020643154311No Hit
GATTAAGAGGGACGGCCGGGGGCAT12440.11741077158885843No Hit
GGGTAGGCACACGCTGAGCCAGTCA12410.11712762664129689No Hit
GTCCTATTCCATTATTCCTAGCTGC12060.1138242689197454No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12000.1132579790246223No Hit
GTACATGGAAGCAGTGGTATCAACG11890.11221978088356326No Hit
GTATCTGATCGTCTTCGAACCTCCG11880.11212539923437606No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC10660.10061083803353946No Hit
ATCAGATACCGTCGTAGTTCCGACC10650.10051645638435229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1200.015.0325041
TTACCCT602.5689007E-514.2487234
CGTTATT1257.2759576E-1213.6729592
GTTATTC1351.8189894E-1213.3653833
CAAGACG3000.013.2988094
ACTACAC604.1030016E-412.6619423
CGTCTTA853.937652E-612.2947615
CGAACGA701.0878123E-412.21723316
ACGAACG701.08973436E-412.21492315
GCGTCCC1251.4024408E-912.16293416
CGTCGTA2350.012.12829310
CACGCCC1501.4551915E-1112.03623816
GCACGCC1501.4551915E-1112.03396115
AAGACGG3000.012.03282455
GTGTAAT951.0463918E-611.992691
ATACCGT2400.011.875626
TAAACTG802.8655728E-511.8744985
CGCATCG2250.011.8228413
ATCGCCA2100.011.76474416
GTTATAT1052.7380702E-711.7547411
Produced by FastQC (version 0.11.5)