##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062626_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1059528 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.066820319991542 32.0 32.0 32.0 32.0 32.0 2 30.629692655597587 32.0 32.0 32.0 32.0 32.0 3 30.62680080186649 32.0 32.0 32.0 32.0 32.0 4 30.711294085668335 32.0 32.0 32.0 32.0 32.0 5 30.445471002182103 32.0 32.0 32.0 32.0 32.0 6 34.093829516539444 36.0 36.0 36.0 32.0 36.0 7 33.953070612574656 36.0 36.0 36.0 32.0 36.0 8 33.92889097786939 36.0 36.0 36.0 32.0 36.0 9 34.14883136641976 36.0 36.0 36.0 32.0 36.0 10 33.73434114058335 36.0 36.0 36.0 32.0 36.0 11 34.15387700938531 36.0 36.0 36.0 32.0 36.0 12 33.86263128487402 36.0 36.0 36.0 32.0 36.0 13 34.03741666100377 36.0 36.0 36.0 32.0 36.0 14 33.91621646620004 36.0 36.0 36.0 32.0 36.0 15 33.841956984619564 36.0 36.0 36.0 32.0 36.0 16 33.845786991943584 36.0 36.0 36.0 32.0 36.0 17 33.742172929832904 36.0 36.0 36.0 32.0 36.0 18 33.77328867193694 36.0 36.0 36.0 32.0 36.0 19 33.76149662868749 36.0 36.0 36.0 32.0 36.0 20 33.74591988130564 36.0 36.0 36.0 32.0 36.0 21 33.728598961046806 36.0 36.0 36.0 32.0 36.0 22 33.68234440241315 36.0 36.0 36.0 27.0 36.0 23 33.65310591131145 36.0 36.0 36.0 27.0 36.0 24 33.615101252633245 36.0 36.0 36.0 27.0 36.0 25 33.14958075671431 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 38.0 5 109.0 6 169.0 7 51.0 8 160.0 9 156.0 10 131.0 11 39.0 12 67.0 13 63.0 14 252.0 15 282.0 16 408.0 17 577.0 18 814.0 19 1171.0 20 1838.0 21 2788.0 22 4002.0 23 5777.0 24 8061.0 25 11348.0 26 15101.0 27 18994.0 28 25590.0 29 33295.0 30 42552.0 31 57377.0 32 81404.0 33 115388.0 34 242709.0 35 388810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.072089640571214 17.222735600525187 10.785695340234522 25.919479418669077 2 17.345261039703857 19.986616252664007 36.012285109684434 26.655837597947702 3 19.33608940234832 23.930041152263374 27.78268584588666 28.95118359950164 4 12.78287046083354 14.881962084762257 35.04653268048818 37.28863477391602 5 15.030302686973574 36.23963434726247 32.89080741389061 15.839255551873347 6 34.82709910617669 34.63673910292848 16.575295124102258 13.960866666792565 7 30.71583391179929 30.11319044043689 20.558255063347925 18.61272058441589 8 28.970745208429765 31.664320867536343 19.419154945542825 19.94577897849107 9 27.71265762038097 13.990662751899757 18.52419711380409 39.77248251391518 10 16.557088619316605 25.966123091518302 30.644655306330332 26.832132982834768 11 37.92350780116111 21.072111604983068 21.666553277697144 19.337827316158684 12 25.135971647962197 23.58624926166568 27.913431777909036 23.36434731246308 13 29.497921590231524 18.889291016110395 25.426714203773926 26.18607318988416 14 23.881818422355693 19.510865251229404 24.436893093790044 32.170423232624856 15 25.424407832139817 26.594523398059444 22.3232803297942 25.65778844000654 16 26.271242490839725 25.30069710707179 23.396927740099436 25.03113266198905 17 24.269685005887123 25.421406445784612 24.88457271588162 25.424335832446644 18 25.40449205663692 24.072392289017262 26.07091994832632 24.45219570601949 19 25.74005347153902 24.730539544650814 25.02502048419244 24.504386499617723 20 25.824845094965266 23.807795855150445 24.738785272374884 25.628573777509406 21 27.1619101980489 23.749235710984877 24.20133569841604 24.887518392550177 22 26.146432575189 23.846005516412177 24.757688097745227 25.249873810653593 23 24.54523629943423 23.627202458928075 25.47451754311347 26.353043698524225 24 24.93767165104441 24.842218076449807 24.94702799152448 25.273082280981303 25 25.045787632330512 24.17322373283082 25.10107300882865 25.67991562601002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 471.0 3 729.0 4 729.0 5 729.0 6 1908.5 7 3088.0 8 3088.0 9 3088.0 10 3123.0 11 3158.0 12 3158.0 13 3158.0 14 3099.0 15 3040.0 16 3040.0 17 3040.0 18 4817.0 19 6594.0 20 6594.0 21 6594.0 22 10417.5 23 14241.0 24 14241.0 25 14241.0 26 21364.5 27 28488.0 28 28488.0 29 28488.0 30 34673.0 31 40858.0 32 40858.0 33 40858.0 34 50879.5 35 60901.0 36 60901.0 37 60901.0 38 69472.5 39 78044.0 40 78044.0 41 78044.0 42 90965.5 43 103887.0 44 103887.0 45 103887.0 46 120043.0 47 136199.0 48 136199.0 49 136199.0 50 138953.5 51 141708.0 52 141708.0 53 141708.0 54 132648.5 55 123589.0 56 123589.0 57 123589.0 58 114425.5 59 105262.0 60 105262.0 61 105262.0 62 95057.0 63 84852.0 64 84852.0 65 84852.0 66 71396.0 67 57940.0 68 57940.0 69 57940.0 70 44087.0 71 30234.0 72 30234.0 73 30234.0 74 23788.5 75 17343.0 76 17343.0 77 17343.0 78 14259.5 79 11176.0 80 11176.0 81 11176.0 82 7759.0 83 4342.0 84 4342.0 85 4342.0 86 3246.5 87 2151.0 88 2151.0 89 2151.0 90 1470.5 91 790.0 92 790.0 93 790.0 94 488.0 95 186.0 96 186.0 97 186.0 98 350.5 99 515.0 100 515.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009155019971156968 2 0.003208976072364298 3 0.004530319160984892 4 0.015572972115885566 5 0.03595940834031758 6 0.04549195490822328 7 0.06540648288671937 8 0.08475472097009235 9 0.09173896300994405 10 0.1157119019034891 11 0.11552313860511473 12 0.1324174538096209 13 0.11958154952016369 14 0.12194109074984334 15 0.11052091119819392 16 0.10891642316201178 17 0.12137480085472022 18 0.12732084475351288 19 0.13166240061612342 20 0.12260176229415362 21 0.127887134648636 22 0.15044434880437327 23 0.14213876367590098 24 0.1342107051441774 25 0.13043543917668998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1059528.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.17464910195157 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8123444158132 38.26394732447815 2 12.909865583674415 12.696762200611012 3 4.050204034061446 5.9750208649884105 4 1.6720831503654296 3.288964087540229 5 0.8896870120819528 2.1875023314846884 6 0.5424711026867619 1.6005495673542154 7 0.3650635570859669 1.2566310623728882 8 0.26191909930645374 1.0303823841195257 9 0.20738520224604612 0.917828509444792 >10 1.037736062672173 9.527053034128553 >50 0.11410158544560933 3.8929888375476707 >100 0.12234967374157414 12.927545959850365 >500 0.009987728345266516 3.2891471365737406 >1k 0.004801792473685825 3.1456766995057555 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3092 0.29182805928677674 No Hit TATCAACGCAGAGTACTTTTTTTTT 1913 0.18055209489508536 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1859 0.17545548583897735 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1581 0.14921738736493986 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 1497 0.1412893288332163 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 1391 0.13128487401937466 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1388 0.13100172907181312 No Hit GAACTACGACGGTATCTGATCGTCT 1357 0.12807589794701035 No Hit GAATAGGACCGCGGTTCTATTTTGT 1338 0.12628264661245384 No Hit GAATAACGCCGCCGCATCGCCAGTC 1253 0.11826020643154311 No Hit GATTAAGAGGGACGGCCGGGGGCAT 1244 0.11741077158885843 No Hit GGGTAGGCACACGCTGAGCCAGTCA 1241 0.11712762664129689 No Hit GTCCTATTCCATTATTCCTAGCTGC 1206 0.1138242689197454 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1200 0.1132579790246223 No Hit GTACATGGAAGCAGTGGTATCAACG 1189 0.11221978088356326 No Hit GTATCTGATCGTCTTCGAACCTCCG 1188 0.11212539923437606 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 1066 0.10061083803353946 No Hit ATCAGATACCGTCGTAGTTCCGACC 1065 0.10051645638435229 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.8876329837437048E-4 5 0.0 0.0 0.0 9.438164918718524E-5 1.8876329837437048E-4 6 0.0 0.0 0.0 9.438164918718524E-5 2.8314494756155573E-4 7 0.0 0.0 0.0 9.438164918718524E-5 2.8314494756155573E-4 8 0.0 0.0 0.0 9.438164918718524E-5 2.8314494756155573E-4 9 9.438164918718524E-5 0.0 0.0 9.438164918718524E-5 2.8314494756155573E-4 10 9.438164918718524E-5 0.0 0.0 1.8876329837437048E-4 2.8314494756155573E-4 11 9.438164918718524E-5 0.0 0.0 1.8876329837437048E-4 2.8314494756155573E-4 12 9.438164918718524E-5 0.0 0.0 1.8876329837437048E-4 3.7752659674874096E-4 13 9.438164918718524E-5 0.0 0.0 2.8314494756155573E-4 3.7752659674874096E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTAT 120 0.0 15.032504 1 TTACCCT 60 2.5689007E-5 14.248723 4 CGTTATT 125 7.2759576E-12 13.672959 2 GTTATTC 135 1.8189894E-12 13.365383 3 CAAGACG 300 0.0 13.298809 4 ACTACAC 60 4.1030016E-4 12.661942 3 CGTCTTA 85 3.937652E-6 12.29476 15 CGAACGA 70 1.0878123E-4 12.217233 16 ACGAACG 70 1.08973436E-4 12.214923 15 GCGTCCC 125 1.4024408E-9 12.162934 16 CGTCGTA 235 0.0 12.128293 10 CACGCCC 150 1.4551915E-11 12.036238 16 GCACGCC 150 1.4551915E-11 12.033961 15 AAGACGG 300 0.0 12.0328245 5 GTGTAAT 95 1.0463918E-6 11.99269 1 ATACCGT 240 0.0 11.87562 6 TAAACTG 80 2.8655728E-5 11.874498 5 CGCATCG 225 0.0 11.82284 13 ATCGCCA 210 0.0 11.764744 16 GTTATAT 105 2.7380702E-7 11.754741 1 >>END_MODULE