FastQCFastQC Report
Thu 2 Feb 2017
SRR4062626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059528
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14980.1413837104824035No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14620.13798597111166483No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13660.12892533278969504No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13350.1259995016648923No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG12780.12061974766122273No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12560.11854335137910467No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12400.1170332449921097No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12250.11561752025430191No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11410.10768946172257836No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10920.10306476091240628No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10840.1023097077189088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT450.001398161324.444267
GTACATA654.9240177E-420.3123321
TAGGACG1201.430999E-820.1665154
CTAGACC951.5969097E-518.5261764
GTCCTAC2400.018.3375231
CTTATGC750.00129038417.6006983
CGTTCAA1002.4827035E-517.59986914
GAGCGAA4750.017.59986916
AGCGAAA4800.017.41653617
AGAGCGA4950.017.33320215
GCGAAAG5250.017.18082218
GTATTAC902.2111839E-417.115021
GTATTAA2450.017.0651231
GTAAACG2600.016.92294927
TGTAGGA2350.016.8517322
CGAAAGC5250.016.76177819
TTAGGAC1206.402617E-616.5006563
GACGGAC5200.016.4998767
AAGACGG5250.016.3427335
TAAACAT1501.8371065E-716.1332135
Produced by FastQC (version 0.11.5)