##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059528
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248319062827978	32.0	32.0	32.0	32.0	32.0
2	31.381830399951678	32.0	32.0	32.0	32.0	32.0
3	31.474387651860074	32.0	32.0	32.0	32.0	32.0
4	31.582556572360524	32.0	32.0	32.0	32.0	32.0
5	31.50980153426809	32.0	32.0	32.0	32.0	32.0
6	35.09461949094314	36.0	36.0	36.0	36.0	36.0
7	35.10514870772646	36.0	36.0	36.0	36.0	36.0
8	35.043718523719996	36.0	36.0	36.0	36.0	36.0
9	35.1716670064406	36.0	36.0	36.0	36.0	36.0
10	35.04095219758232	36.0	36.0	36.0	36.0	36.0
11	35.188524512801926	36.0	36.0	36.0	36.0	36.0
12	35.08563247030753	36.0	36.0	36.0	36.0	36.0
13	35.13924879757779	36.0	36.0	36.0	36.0	36.0
14	35.09168327783692	36.0	36.0	36.0	36.0	36.0
15	35.05331902507532	36.0	36.0	36.0	36.0	36.0
16	35.068807053706934	36.0	36.0	36.0	36.0	36.0
17	35.03874555462432	36.0	36.0	36.0	36.0	36.0
18	35.047217251455365	36.0	36.0	36.0	36.0	36.0
19	35.03665500109483	36.0	36.0	36.0	36.0	36.0
20	35.02590870651837	36.0	36.0	36.0	36.0	36.0
21	35.01695660709297	36.0	36.0	36.0	36.0	36.0
22	35.00455013930731	36.0	36.0	36.0	36.0	36.0
23	34.96095525554775	36.0	36.0	36.0	32.0	36.0
24	34.93984113680809	36.0	36.0	36.0	32.0	36.0
25	34.9125874917888	36.0	36.0	36.0	32.0	36.0
26	34.86915116919987	36.0	36.0	36.0	32.0	36.0
27	34.84781053450216	36.0	36.0	36.0	32.0	36.0
28	34.83641300654631	36.0	36.0	36.0	32.0	36.0
29	34.813557546379144	36.0	36.0	36.0	32.0	36.0
30	34.79578548183719	36.0	36.0	36.0	32.0	36.0
31	34.801958041694036	36.0	36.0	36.0	32.0	36.0
32	34.75475400366956	36.0	36.0	36.0	32.0	36.0
33	34.726330026200344	36.0	36.0	36.0	32.0	36.0
34	34.70969431671461	36.0	36.0	36.0	32.0	36.0
35	34.6822575712959	36.0	36.0	36.0	32.0	36.0
36	34.65545035147726	36.0	36.0	36.0	32.0	36.0
37	34.6477469212706	36.0	36.0	36.0	32.0	36.0
38	34.60998859869678	36.0	36.0	36.0	32.0	36.0
39	34.59465535596983	36.0	36.0	36.0	32.0	36.0
40	34.58067932135819	36.0	36.0	36.0	32.0	36.0
41	34.54990429700772	36.0	36.0	36.0	32.0	36.0
42	34.50867178592732	36.0	36.0	36.0	32.0	36.0
43	34.49485903156877	36.0	36.0	36.0	32.0	36.0
44	34.453989889837736	36.0	36.0	36.0	32.0	36.0
45	34.40987213174168	36.0	36.0	36.0	32.0	36.0
46	34.40980700840374	36.0	36.0	36.0	32.0	36.0
47	34.36788267983479	36.0	36.0	36.0	32.0	36.0
48	34.332001608263305	36.0	36.0	36.0	32.0	36.0
49	34.31411628574233	36.0	36.0	36.0	32.0	36.0
50	33.78157726836856	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	5.0
21	22.0
22	105.0
23	303.0
24	757.0
25	1635.0
26	3545.0
27	6641.0
28	11369.0
29	17245.0
30	24701.0
31	35946.0
32	52835.0
33	86000.0
34	188501.0
35	629914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52516365936198	17.868251263728745	11.258631349912447	26.34795372699683
2	16.763946909905027	20.415637075476507	35.74274975884442	27.077666255774048
3	18.742998015140806	24.158464060646274	28.23898724046055	28.859550683752378
4	12.509249401620345	15.336546084671665	35.235501091051866	36.91870342265612
5	14.734296781208236	36.43669634025717	32.76534456852485	16.06366231000974
6	35.13756610260994	34.783823347588125	16.275375710104605	13.803234839697337
7	30.629865374015598	30.155597586849993	20.61786002823899	18.59667701089542
8	29.00923807582244	31.808880935661914	19.264710323842316	19.917170664673325
9	27.827391064700507	13.643339298253562	18.443401212615427	40.0858684244305
10	16.495458355041112	26.090202429761177	30.493767035887675	26.92057217931003
11	38.28667104597519	20.85154899162646	21.70966694603635	19.152113016362
12	25.119581549520163	23.470167848324913	27.916298578234837	23.493952023920084
13	29.559294327285357	18.81460423886863	25.48181831910058	26.14428311474543
14	23.924615489161212	19.3178471923347	24.49864467951767	32.25889263898642
15	25.519665360424643	26.593917291473186	22.157791016377104	25.728626331725067
16	26.42006629367039	25.25511359775296	23.33586276153155	24.988957347045098
17	24.467121208689154	25.315612234881947	24.729785338377088	25.487481218051812
18	25.66954341933389	23.876575229724935	26.10483158538519	24.349049765555982
19	25.87482350631602	24.531583875084	25.227837301137868	24.365755317462114
20	25.92418510884092	23.577574165099932	24.852009574074494	25.646231151984654
21	27.387761342786597	23.639111000369976	24.227958109648824	24.7451695471946
22	26.248574837097273	23.677618713238346	24.847573636562696	25.226232813101685
23	24.696374234564825	23.29631685052212	25.576860639832077	26.43044827508098
24	25.028031349808593	24.517898535951858	25.097401861961178	25.356668252278375
25	25.132606217107995	23.86534381347166	25.254547307857838	25.747502661562503
26	24.813124334609373	24.89476446115629	25.71248706971406	24.579624134520277
27	25.823007980912255	24.140466320852305	24.78339411511541	25.25313158312003
28	24.722140424792926	23.97595910631904	25.82517875884356	25.476721710044476
29	24.725915690760413	24.342254286814505	25.558833744837322	25.372996277587756
30	24.588590391193062	24.507988462787203	25.907196411987226	24.996224734032513
31	25.165545412674323	24.514312033282746	24.677969812973323	25.642172741069608
32	25.115900665201863	24.172178554979197	24.67976306430788	26.032157715511055
33	24.56641070363407	23.815227157753263	25.392722042267877	26.225640096344787
34	25.380641191171915	24.11045295641078	25.620842488353308	24.888063364064
35	26.11493042184822	23.873271872003382	25.394609675251623	24.617188030896777
36	24.749039194811274	24.684765291714804	25.016516788607756	25.549678724866165
37	25.90398743591486	24.466649300443216	24.75007739295233	24.87928587068959
38	24.79141655529632	24.113001260938834	25.44255555303871	25.653026630726135
39	25.36978730151539	24.042592550645193	24.77376718689832	25.813852960941098
40	25.590451597315027	24.305728588579065	25.408295014383764	24.69552479972214
41	24.417665224515066	24.779618849147923	25.790163166995118	25.012552759341894
42	26.141451665269816	24.77471100339019	24.998395511963817	24.085441819376175
43	25.01094827130571	23.67054008954931	25.41641183621386	25.902099802931115
44	24.601803822079265	24.4262539545911	25.120997274257974	25.850944949071664
45	24.58717466645525	24.75460771211332	25.236614794512274	25.421602826919155
46	24.56558445419513	24.406928752169602	25.31922263425094	25.70826415938433
47	24.83407706072893	24.06420594830906	25.80101705665164	25.300699934310373
48	25.679359110849358	25.130246675878315	24.393597903972335	24.79679630929999
49	24.300820742821333	25.075222174402185	24.96847652917148	25.655480553605003
50	24.250609705453748	25.481723937451395	24.77310651535401	25.49455984174085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	50.0
2	67.0
3	181.5
4	296.0
5	327.0
6	358.0
7	298.5
8	239.0
9	347.5
10	456.0
11	648.5
12	841.0
13	1398.5
14	1956.0
15	2660.0
16	3364.0
17	3692.0
18	4020.0
19	4273.0
20	4526.0
21	4978.5
22	5431.0
23	5520.0
24	5609.0
25	6285.5
26	6962.0
27	8151.5
28	9341.0
29	10468.5
30	11596.0
31	12720.0
32	13844.0
33	15850.5
34	17857.0
35	20883.0
36	23909.0
37	29975.5
38	36042.0
39	38816.0
40	41590.0
41	46276.5
42	50963.0
43	52240.5
44	53518.0
45	59936.5
46	66355.0
47	71567.0
48	76779.0
49	79302.0
50	81825.0
51	78010.0
52	74195.0
53	73490.5
54	72786.0
55	74881.5
56	76977.0
57	76196.5
58	75416.0
59	69346.5
60	63277.0
61	56151.0
62	49025.0
63	42915.5
64	36806.0
65	31803.0
66	26800.0
67	23626.5
68	20453.0
69	18897.0
70	17341.0
71	13399.5
72	9458.0
73	8554.5
74	7651.0
75	5507.0
76	3363.0
77	3114.0
78	2865.0
79	2470.5
80	2076.0
81	1665.0
82	1254.0
83	1104.0
84	954.0
85	806.5
86	659.0
87	470.0
88	281.0
89	175.5
90	70.0
91	46.5
92	23.0
93	19.5
94	16.0
95	9.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015384208817511194
2	0.004341555862610521
3	6.606715443102967E-4
4	0.0
5	0.0
6	9.438164918718524E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.438164918718524E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1059528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.15039458259068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32574723562554	40.84718624314133
2	12.828536515667881	13.380264824185062
3	3.921593552209342	6.135379534207818
4	1.663115904333733	3.469286025903452
5	0.8305657511621165	2.16571658249451
6	0.5121790571425865	1.6026203956155087
7	0.3388512529287937	1.2369858581529278
8	0.2447072281680284	1.0209262804939783
9	0.1773038037988503	0.8321816996193854
>10	0.9364846045455588	8.942128442646617
>50	0.10355079067110846	3.7461613971078584
>100	0.105354933488945	11.602654742767612
>500	0.009461928081611328	3.4006782561016795
>1k	0.0025474421758184345	1.6178297175622272
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1498	0.1413837104824035	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1462	0.13798597111166483	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1366	0.12892533278969504	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1335	0.1259995016648923	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1278	0.12061974766122273	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1256	0.11854335137910467	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1240	0.1170332449921097	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1225	0.11561752025430191	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1141	0.10768946172257836	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1092	0.10306476091240628	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1084	0.1023097077189088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.438164918718524E-5	0.0	0.0	9.438164918718524E-5	0.0
6	9.438164918718524E-5	0.0	0.0	9.438164918718524E-5	0.0
7	9.438164918718524E-5	0.0	0.0	9.438164918718524E-5	0.0
8	9.438164918718524E-5	0.0	0.0	9.438164918718524E-5	0.0
9	9.438164918718524E-5	0.0	0.0	9.438164918718524E-5	9.438164918718524E-5
10	9.438164918718524E-5	0.0	0.0	1.8876329837437048E-4	9.438164918718524E-5
11	9.438164918718524E-5	0.0	0.0	1.8876329837437048E-4	9.438164918718524E-5
12	9.438164918718524E-5	0.0	0.0	1.8876329837437048E-4	9.438164918718524E-5
13	9.438164918718524E-5	0.0	0.0	1.8876329837437048E-4	9.438164918718524E-5
14	9.438164918718524E-5	0.0	0.0	3.7752659674874096E-4	9.438164918718524E-5
15	9.438164918718524E-5	0.0	0.0	3.7752659674874096E-4	9.438164918718524E-5
16	9.438164918718524E-5	0.0	0.0	3.7752659674874096E-4	1.8876329837437048E-4
17	9.438164918718524E-5	0.0	0.0	4.719082459359262E-4	1.8876329837437048E-4
18	9.438164918718524E-5	0.0	0.0	4.719082459359262E-4	1.8876329837437048E-4
19	9.438164918718524E-5	0.0	0.0	5.662898951231115E-4	1.8876329837437048E-4
20	9.438164918718524E-5	0.0	0.0	7.550531934974819E-4	1.8876329837437048E-4
21	9.438164918718524E-5	0.0	0.0	0.0010381981410590377	1.8876329837437048E-4
22	9.438164918718524E-5	0.0	0.0	0.0016044880361821491	1.8876329837437048E-4
23	9.438164918718524E-5	0.0	0.0	0.002831449475615557	1.8876329837437048E-4
24	9.438164918718524E-5	0.0	0.0	0.004152792564236151	1.8876329837437048E-4
25	9.438164918718524E-5	0.0	0.0	0.005285372354482373	2.8314494756155573E-4
26	9.438164918718524E-5	0.0	0.0	0.007267386987413263	2.8314494756155573E-4
27	9.438164918718524E-5	0.0	0.0	0.009815691515467264	2.8314494756155573E-4
28	9.438164918718524E-5	0.0	0.0	0.015950498712634306	2.8314494756155573E-4
29	9.438164918718524E-5	0.0	0.0	0.03303357721551483	2.8314494756155573E-4
30	9.438164918718524E-5	0.0	0.0	0.0620087435159807	2.8314494756155573E-4
31	9.438164918718524E-5	0.0	0.0	0.12363996043521266	2.8314494756155573E-4
32	9.438164918718524E-5	0.0	0.0	0.19008464146299106	2.8314494756155573E-4
33	9.438164918718524E-5	0.0	0.0	0.2610596416517544	2.8314494756155573E-4
34	9.438164918718524E-5	0.0	0.0	0.3385469756344334	2.8314494756155573E-4
35	9.438164918718524E-5	0.0	0.0	0.424434276394772	2.8314494756155573E-4
36	9.438164918718524E-5	0.0	0.0	0.5434495360198126	2.8314494756155573E-4
37	9.438164918718524E-5	0.0	0.0	0.7212645630884695	2.8314494756155573E-4
38	9.438164918718524E-5	0.0	0.0	0.9503288256657682	2.8314494756155573E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGT	45	0.0013981613	24.44426	7
GTACATA	65	4.9240177E-4	20.312332	1
TAGGACG	120	1.430999E-8	20.166515	4
CTAGACC	95	1.5969097E-5	18.526176	4
GTCCTAC	240	0.0	18.337523	1
CTTATGC	75	0.001290384	17.600698	3
CGTTCAA	100	2.4827035E-5	17.599869	14
GAGCGAA	475	0.0	17.599869	16
AGCGAAA	480	0.0	17.416536	17
AGAGCGA	495	0.0	17.333202	15
GCGAAAG	525	0.0	17.180822	18
GTATTAC	90	2.2111839E-4	17.11502	1
GTATTAA	245	0.0	17.065123	1
GTAAACG	260	0.0	16.922949	27
TGTAGGA	235	0.0	16.851732	2
CGAAAGC	525	0.0	16.761778	19
TTAGGAC	120	6.402617E-6	16.500656	3
GACGGAC	520	0.0	16.499876	7
AAGACGG	525	0.0	16.342733	5
TAAACAT	150	1.8371065E-7	16.133213	5
>>END_MODULE