FastQCFastQC Report
Thu 2 Feb 2017
SRR4062625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5691
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTT571.0015814443858724No Hit
ACGCAGAGTACATGGGAATAACGCC260.45686171147425764No Hit
GTACATGGAAGCAGTGGTATCAACG260.45686171147425764No Hit
ACGCAGAGTACATGGGGAATAATTG240.42171850289931473No Hit
GAGTACATGGAAGCAGTGGTATCAA200.3514320857494289No Hit
TCCATGTACTCTGCGTTGATACCAC180.316288877174486No Hit
ACGCAGAGTACATGGAAGCAGTGGT130.22843085573712882No Hit
CATGTACTCTGCGTTGATACCACTG100.17571604287471446No Hit
CCGCAGCTAGGAATAATGGAATAGG90.158144438587243No Hit
CTCCACTCCTGGTGGTGCCCTTCCG90.158144438587243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG80.14057283429977158No Hit
CTCCCGACCCGGGGAGGTAGTGACG80.14057283429977158No Hit
GTCCTACAGTGGACATTTCTAAATT80.14057283429977158No Hit
GCGCAAGACGGACCAGAGCGAAAGC80.14057283429977158No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG80.14057283429977158No Hit
GTACATGGGAAGCAGTGGTATCAAC70.12300123001230012No Hit
CTTTAGGACGTGAAATATGGCGAGG70.12300123001230012No Hit
GTCCTAAAGTGTGTATTTCTCATTT70.12300123001230012No Hit
GTATCAACGCAGAGTACTTTTTTTT70.12300123001230012No Hit
ACGCAGAGTACATGGGAGAAATCGT70.12300123001230012No Hit
GGCGGTGACTCGCCTCGCGGCGGAC70.12300123001230012No Hit
ACGCAGAGTACATGGGAATTCCTCG70.12300123001230012No Hit
GGATTGACAGATTGATAGCTCTTTC70.12300123001230012No Hit
ATATTAAAGTTGCTGCAGTTAAAAA70.12300123001230012No Hit
CCCAAGATCCAACTACGAGCTTTTT70.12300123001230012No Hit
GATTTAAAGTGGACTCATTCCAATT60.10542962572482868No Hit
ACGCAGAGTACATGGGAAACCAGAA60.10542962572482868No Hit
GTCAATCCTGTCCGTGTCCGGGCCG60.10542962572482868No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT60.10542962572482868No Hit
GCTTCCATGTACTCTGCGTTGATAC60.10542962572482868No Hit
TATCAACGCAGAGTACTTTTTTTTT60.10542962572482868No Hit
CATCCAAGGAAGGCAGCAGGCGCGC60.10542962572482868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGA701.3218596E-816.2857151
CAGAGTA701.3218596E-816.2857154
AGAGTAC701.3218596E-816.2857155
GCAGAGT701.3218596E-816.2857153
CGCAGAG701.3218596E-816.2857152
CATGGGA455.4066215E-414.77777911
ACATGGG652.1679225E-614.61538610
AGTACAT755.7039324E-713.9333327
GAGTACA755.7039324E-713.9333326
GTACATG904.4629633E-611.6111128
TACATGG904.4629633E-611.6111129
Produced by FastQC (version 0.11.5)