##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062625_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5691
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.346863468634687	32.0	32.0	32.0	14.0	32.0
2	28.266034088912317	32.0	32.0	32.0	14.0	32.0
3	28.694429801440872	32.0	32.0	32.0	14.0	32.0
4	28.355824986821297	32.0	32.0	32.0	14.0	32.0
5	28.15181866104375	32.0	27.0	32.0	14.0	32.0
6	31.9367422245651	36.0	32.0	36.0	14.0	36.0
7	31.584080126515552	36.0	32.0	36.0	14.0	36.0
8	31.31804603760323	36.0	32.0	36.0	14.0	36.0
9	31.795290810050957	36.0	32.0	36.0	14.0	36.0
10	30.880161658759445	36.0	32.0	36.0	14.0	36.0
11	31.924793533649623	36.0	32.0	36.0	14.0	36.0
12	31.319100333860483	36.0	32.0	36.0	14.0	36.0
13	31.656826568265682	36.0	32.0	36.0	14.0	36.0
14	31.30521876647338	36.0	32.0	36.0	14.0	36.0
15	31.12335266209805	36.0	32.0	36.0	14.0	36.0
16	31.25267966965384	36.0	32.0	36.0	14.0	36.0
17	31.034440344403443	36.0	32.0	36.0	14.0	36.0
18	31.155157265858374	36.0	32.0	36.0	14.0	36.0
19	30.88771744860306	36.0	32.0	36.0	14.0	36.0
20	31.086276577051486	36.0	32.0	36.0	14.0	36.0
21	30.95853101388157	36.0	32.0	36.0	14.0	36.0
22	30.884203127745565	36.0	32.0	36.0	14.0	36.0
23	30.684941135125637	36.0	32.0	36.0	14.0	36.0
24	30.7387102442453	36.0	32.0	36.0	14.0	36.0
25	30.051660516605168	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	1.0
8	0.0
9	0.0
10	1.0
11	0.0
12	0.0
13	2.0
14	5.0
15	3.0
16	20.0
17	48.0
18	64.0
19	90.0
20	136.0
21	87.0
22	132.0
23	160.0
24	123.0
25	153.0
26	212.0
27	206.0
28	222.0
29	254.0
30	312.0
31	384.0
32	471.0
33	585.0
34	990.0
35	1028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.056931997891404	26.26954840976981	9.892813213846424	27.780706378492354
2	12.370409418379898	18.924617817606748	34.352486382006674	34.352486382006674
3	28.799859427165703	21.595501669302408	24.3718151467229	25.232823756808997
4	9.876977152899824	13.181019332161686	32.74165202108963	44.200351493848856
5	12.4274916505537	43.55774301283178	30.093162242924944	13.921603093689578
6	40.770042194092824	32.419127988748244	15.295358649789028	11.515471167369903
7	26.92104800422015	36.85598733954633	19.922630560928432	16.300334095305082
8	33.08125219838199	31.5511783327471	18.571931058740766	16.795638410130145
9	24.090029892737824	12.871461227360648	26.446280991735538	36.59222788816599
10	14.016883573689764	32.641575800211044	31.445655997186073	21.89588462891312
11	32.6530612244898	20.285010555946517	19.739619985925405	27.32230823363828
12	21.160949868073878	29.90325417766051	29.199648197009676	19.736147757255935
13	27.563764291996485	17.959542656112575	32.17238346525945	22.304309586631486
14	28.682034136899524	18.08903748020412	25.092380784796763	28.136547598099593
15	30.31315974665728	26.583391977480648	21.79802955665025	21.305418719211822
16	29.950756243404854	24.49876890608512	24.059092507914176	21.49138234259585
17	23.43007915567282	28.566402814423924	26.36763412489006	21.63588390501319
18	24.02323125659979	25.448785638859555	28.563885955649422	21.964097148891234
19	23.76847290640394	26.952850105559467	27.023223082336383	22.25545390570021
20	23.75505894773887	24.617279605841986	28.206932957944748	23.420728488474396
21	24.010554089709764	26.666666666666668	25.505716798592786	23.817062445030782
22	23.07286166842661	26.891939457937347	27.402323125659976	22.632875747976065
23	21.602112676056336	26.672535211267608	27.27112676056338	24.454225352112676
24	23.319253783878917	25.677578317493836	27.067933826117564	23.93523407250968
25	22.748064743138634	25.95003518648839	26.548205489092187	24.75369458128079
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	2.5
3	5.0
4	5.0
5	5.0
6	7.5
7	10.0
8	10.0
9	10.0
10	12.5
11	15.0
12	15.0
13	15.0
14	19.0
15	23.0
16	23.0
17	23.0
18	39.5
19	56.0
20	56.0
21	56.0
22	108.0
23	160.0
24	160.0
25	160.0
26	178.5
27	197.0
28	197.0
29	197.0
30	261.0
31	325.0
32	325.0
33	325.0
34	346.5
35	368.0
36	368.0
37	368.0
38	428.0
39	488.0
40	488.0
41	488.0
42	545.0
43	602.0
44	602.0
45	602.0
46	715.0
47	828.0
48	828.0
49	828.0
50	823.5
51	819.0
52	819.0
53	819.0
54	678.0
55	537.0
56	537.0
57	537.0
58	485.0
59	433.0
60	433.0
61	433.0
62	372.0
63	311.0
64	311.0
65	311.0
66	273.5
67	236.0
68	236.0
69	236.0
70	172.0
71	108.0
72	108.0
73	108.0
74	91.5
75	75.0
76	75.0
77	75.0
78	60.5
79	46.0
80	46.0
81	46.0
82	36.0
83	26.0
84	26.0
85	26.0
86	18.0
87	10.0
88	10.0
89	10.0
90	7.0
91	4.0
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	4.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.017571604287471447
5	0.035143208574942894
6	0.05271481286241434
7	0.07028641714988579
8	0.08785802143735723
9	0.07028641714988579
10	0.08785802143735723
11	0.12300123001230012
12	0.10542962572482868
13	0.10542962572482868
14	0.14057283429977158
15	0.12300123001230012
16	0.08785802143735723
17	0.10542962572482868
18	0.158144438587243
19	0.12300123001230012
20	0.14057283429977158
21	0.10542962572482868
22	0.158144438587243
23	0.1932876471621859
24	0.158144438587243
25	0.12300123001230012
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5691.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.7845721314356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.01515772224498	79.79265506940784
2	4.240065546907005	7.2746441750131785
3	1.1061040557148711	2.8465998945703745
4	0.6145022531749283	2.1085925144965736
5	0.36870135190495695	1.58144438587243
6	0.14338385907414994	0.7380073800738007
7	0.20483408439164277	1.2300123001230012
8	0.10241704219582139	0.7028641714988578
9	0.04096681687832855	0.316288877174486
>10	0.14338385907414994	2.4073097873835883
>50	0.020483408439164276	1.0015814443858724
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACTTTTTTTTTTTTTT	57	1.0015814443858724	No Hit
ACGCAGAGTACATGGGAATAACGCC	26	0.45686171147425764	No Hit
GTACATGGAAGCAGTGGTATCAACG	26	0.45686171147425764	No Hit
ACGCAGAGTACATGGGGAATAATTG	24	0.42171850289931473	No Hit
GAGTACATGGAAGCAGTGGTATCAA	20	0.3514320857494289	No Hit
TCCATGTACTCTGCGTTGATACCAC	18	0.316288877174486	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	13	0.22843085573712882	No Hit
CATGTACTCTGCGTTGATACCACTG	10	0.17571604287471446	No Hit
CCGCAGCTAGGAATAATGGAATAGG	9	0.158144438587243	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	9	0.158144438587243	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	8	0.14057283429977158	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	8	0.14057283429977158	No Hit
GTCCTACAGTGGACATTTCTAAATT	8	0.14057283429977158	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	8	0.14057283429977158	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	8	0.14057283429977158	No Hit
GTACATGGGAAGCAGTGGTATCAAC	7	0.12300123001230012	No Hit
CTTTAGGACGTGAAATATGGCGAGG	7	0.12300123001230012	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7	0.12300123001230012	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7	0.12300123001230012	No Hit
ACGCAGAGTACATGGGAGAAATCGT	7	0.12300123001230012	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	7	0.12300123001230012	No Hit
ACGCAGAGTACATGGGAATTCCTCG	7	0.12300123001230012	No Hit
GGATTGACAGATTGATAGCTCTTTC	7	0.12300123001230012	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	7	0.12300123001230012	No Hit
CCCAAGATCCAACTACGAGCTTTTT	7	0.12300123001230012	No Hit
GATTTAAAGTGGACTCATTCCAATT	6	0.10542962572482868	No Hit
ACGCAGAGTACATGGGAAACCAGAA	6	0.10542962572482868	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	6	0.10542962572482868	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	6	0.10542962572482868	No Hit
GCTTCCATGTACTCTGCGTTGATAC	6	0.10542962572482868	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6	0.10542962572482868	No Hit
CATCCAAGGAAGGCAGCAGGCGCGC	6	0.10542962572482868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAGA	70	1.3218596E-8	16.285715	1
CAGAGTA	70	1.3218596E-8	16.285715	4
AGAGTAC	70	1.3218596E-8	16.285715	5
GCAGAGT	70	1.3218596E-8	16.285715	3
CGCAGAG	70	1.3218596E-8	16.285715	2
CATGGGA	45	5.4066215E-4	14.777779	11
ACATGGG	65	2.1679225E-6	14.615386	10
AGTACAT	75	5.7039324E-7	13.933332	7
GAGTACA	75	5.7039324E-7	13.933332	6
GTACATG	90	4.4629633E-6	11.611112	8
TACATGG	90	4.4629633E-6	11.611112	9
>>END_MODULE