##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5691
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.94447373045159	32.0	32.0	32.0	21.0	32.0
2	29.718678615357582	32.0	32.0	32.0	14.0	32.0
3	30.807063784923564	32.0	32.0	32.0	32.0	32.0
4	31.1396942540854	32.0	32.0	32.0	32.0	32.0
5	30.940080829379724	32.0	32.0	32.0	32.0	32.0
6	34.49007204357758	36.0	36.0	36.0	32.0	36.0
7	34.669302407309786	36.0	36.0	36.0	32.0	36.0
8	34.35828501142154	36.0	36.0	36.0	32.0	36.0
9	34.6136004217185	36.0	36.0	36.0	32.0	36.0
10	34.47706905640485	36.0	36.0	36.0	32.0	36.0
11	34.829555438411525	36.0	36.0	36.0	32.0	36.0
12	34.54261114039712	36.0	36.0	36.0	32.0	36.0
13	34.73888596028817	36.0	36.0	36.0	32.0	36.0
14	34.66403092602354	36.0	36.0	36.0	32.0	36.0
15	34.54788262168336	36.0	36.0	36.0	32.0	36.0
16	34.55543841152697	36.0	36.0	36.0	32.0	36.0
17	34.56281848532771	36.0	36.0	36.0	32.0	36.0
18	34.60235459497452	36.0	36.0	36.0	32.0	36.0
19	34.60446318748902	36.0	36.0	36.0	32.0	36.0
20	34.55350553505535	36.0	36.0	36.0	32.0	36.0
21	34.52099806712353	36.0	36.0	36.0	32.0	36.0
22	34.56896854682832	36.0	36.0	36.0	32.0	36.0
23	34.485854858548585	36.0	36.0	36.0	32.0	36.0
24	34.345809172377436	36.0	36.0	36.0	32.0	36.0
25	34.30117729748726	36.0	36.0	36.0	32.0	36.0
26	34.47777192057635	36.0	36.0	36.0	32.0	36.0
27	34.32735898787559	36.0	36.0	36.0	32.0	36.0
28	34.2925672113864	36.0	36.0	36.0	32.0	36.0
29	34.22684941135125	36.0	36.0	36.0	32.0	36.0
30	34.18221753646108	36.0	36.0	36.0	32.0	36.0
31	34.19416622737656	36.0	36.0	36.0	32.0	36.0
32	34.03953610964681	36.0	36.0	36.0	32.0	36.0
33	34.13881567387102	36.0	36.0	36.0	32.0	36.0
34	33.99349850641364	36.0	36.0	36.0	32.0	36.0
35	33.89527323844667	36.0	36.0	36.0	32.0	36.0
36	33.82041820418204	36.0	36.0	36.0	32.0	36.0
37	33.89527323844667	36.0	36.0	36.0	32.0	36.0
38	33.88473027587419	36.0	36.0	36.0	32.0	36.0
39	33.76541908276226	36.0	36.0	36.0	32.0	36.0
40	33.81075382182393	36.0	36.0	36.0	32.0	36.0
41	33.63626779124934	36.0	36.0	36.0	27.0	36.0
42	33.68195396239677	36.0	36.0	36.0	27.0	36.0
43	33.88824459673168	36.0	36.0	36.0	32.0	36.0
44	33.608504656475134	36.0	36.0	36.0	27.0	36.0
45	33.459848884203126	36.0	36.0	36.0	21.0	36.0
46	33.519592338780534	36.0	36.0	36.0	21.0	36.0
47	33.51203654893692	36.0	36.0	36.0	21.0	36.0
48	33.44192584782991	36.0	36.0	36.0	21.0	36.0
49	33.322087506589355	36.0	36.0	36.0	21.0	36.0
50	32.67650676506765	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	3.0
23	2.0
24	5.0
25	22.0
26	33.0
27	58.0
28	112.0
29	152.0
30	200.0
31	351.0
32	494.0
33	816.0
34	1282.0
35	2159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51669595782074	18.38312829525483	12.1792618629174	24.920913884007028
2	17.817606747496047	21.454928835002633	37.83166403092602	22.895800386575292
3	22.913371990862768	23.66895097522404	28.465998945703742	24.95167808820945
4	14.81286241433843	16.710595677385346	36.30293445791601	32.17360745036022
5	17.712177121771216	34.633632050606224	33.06975926902126	14.584431558601299
6	33.24547531189598	37.25180108943947	16.51730803022316	12.9854155684414
7	29.731154454401686	29.098576700052714	21.26164118784045	19.90862765770515
8	28.16728167281673	34.54577402916886	18.169038833245477	19.117905464768935
9	25.88297311544544	14.074855034264628	18.942189421894216	41.09998242839571
10	16.148304340186257	26.673695308381657	31.94517659462309	25.232823756808997
11	38.569671410999824	19.627481989105604	22.31593744508874	19.486909154805833
12	23.93252503953611	23.194517659462306	30.311017395888246	22.561939905113338
13	30.59216306448779	18.977332630469164	25.232823756808997	25.197680548234054
14	21.964505359339306	20.752064663503777	26.550694078369354	30.73273589878756
15	25.03953610964681	28.90528905289053	22.263222632226324	23.79195220523634
16	24.740818836759797	25.30311017395888	26.322263222632223	23.633807766649095
17	22.878228782287824	27.499560709892812	26.56826568265683	23.053944825162535
18	24.125812686698296	24.51238798102267	28.079423651379372	23.282375680899666
19	25.074679318221754	25.88297311544544	25.197680548234054	23.844667018098754
20	24.969249692496927	25.14496573537164	26.35740643120717	23.528378140924268
21	24.81110525390968	26.023545949745213	24.459673168160254	24.705675628184853
22	25.232823756808997	26.3398348269197	25.162537339659114	23.264804076612194
23	23.33509049376208	25.68968546828325	26.533122474081882	24.442101563872782
24	23.10665963802495	26.884554559831315	25.795115094008082	24.213670708135655
25	23.827095413811282	26.005974345457737	26.462836056931998	23.704094183798983
26	22.84308557371288	26.128975575470044	27.09541381128097	23.93252503953611
27	24.934106483921983	26.410121244069586	25.355824986821297	23.299947285187137
28	21.542786856439992	26.26954840976981	27.253558249868213	24.934106483921983
29	23.95009664382358	26.550694078369354	26.005974345457737	23.493234932349324
30	23.35266209804955	26.533122474081882	27.376559479880513	22.73765594798805
31	23.897381830961166	25.39096819539624	26.8318397469689	23.879810226673694
32	23.633807766649095	26.287120014057287	25.443683008258656	24.635389211034965
33	23.036373220875067	25.39096819539624	26.49797926550694	25.074679318221754
34	23.036373220875067	26.91969776840625	27.21841504129327	22.82551396942541
35	25.742400281145667	25.759971885433142	25.953259532595325	22.544368300825866
36	22.913371990862768	26.989984185556143	26.726410121244072	23.370233702337025
37	24.705675628184853	25.40853979968371	25.373396591108765	24.51238798102267
38	23.704094183798983	26.62098049551924	24.740818836759797	24.934106483921983
39	23.018801616587595	26.25197680548234	26.35740643120717	24.3718151467229
40	25.566684238270952	25.40853979968371	25.42611140397118	23.59866455807415
41	23.07151642945001	26.603408891231766	27.288701458443153	23.036373220875067
42	26.023545949745213	26.65612370409418	24.652960815322437	22.667369530838165
43	24.020383060973465	26.058689158320153	26.44526445264453	23.475663328061852
44	23.036373220875067	26.972412581268667	25.63697065542084	24.354243542435423
45	23.370233702337025	27.358987875593044	25.98840274117027	23.282375680899666
46	23.141802846599894	25.759971885433142	27.077842206993495	24.020383060973465
47	24.934106483921983	26.041117554032684	26.181690388332456	22.84308557371288
48	23.95009664382358	28.16728167281673	25.074679318221754	22.807942365137936
49	23.563521349499208	26.35740643120717	25.812686698295558	24.266385520998067
50	23.229660868037254	27.077842206993495	25.654542259708315	24.037954665260937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	1.5
10	2.0
11	2.5
12	3.0
13	4.0
14	5.0
15	6.0
16	7.0
17	14.5
18	22.0
19	20.5
20	19.0
21	25.5
22	32.0
23	36.0
24	40.0
25	52.5
26	65.0
27	87.5
28	110.0
29	119.5
30	129.0
31	154.0
32	179.0
33	182.0
34	185.0
35	229.5
36	274.0
37	269.5
38	265.0
39	273.5
40	282.0
41	298.0
42	314.0
43	291.5
44	269.0
45	327.0
46	385.0
47	393.5
48	402.0
49	387.0
50	372.0
51	348.5
52	325.0
53	316.5
54	308.0
55	310.0
56	312.0
57	310.5
58	309.0
59	269.0
60	229.0
61	219.0
62	209.0
63	175.5
64	142.0
65	127.0
66	112.0
67	108.0
68	104.0
69	96.0
70	88.0
71	74.0
72	60.0
73	48.5
74	37.0
75	29.0
76	21.0
77	17.5
78	14.0
79	15.5
80	17.0
81	13.0
82	9.0
83	8.0
84	7.0
85	8.0
86	9.0
87	7.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	4.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017571604287471447
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5691.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.14549288350027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.23264683447749	87.7525918116324
2	3.1464530892448517	5.798629414865577
3	0.7818459191456903	2.161307327358988
4	0.4767353165522502	1.7571604287471447
5	0.09534706331045004	0.4392901071867862
6	0.09534706331045004	0.5271481286241434
7	0.057208237986270026	0.36900369003690037
8	0.03813882532418002	0.28114566859954315
9	0.01906941266209001	0.158144438587243
>10	0.057208237986270026	0.7555789843612721
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	19	0.33386048146195746	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	13	0.22843085573712882	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	11	0.1932876471621859	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9	0.158144438587243	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	8	0.14057283429977158	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	8	0.14057283429977158	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	7	0.12300123001230012	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	7	0.12300123001230012	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	7	0.12300123001230012	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	6	0.10542962572482868	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	6	0.10542962572482868	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	6	0.10542962572482868	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	6	0.10542962572482868	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC	6	0.10542962572482868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.017571604287471447	0.0
32	0.0	0.0	0.0	0.035143208574942894	0.0
33	0.0	0.0	0.0	0.035143208574942894	0.0
34	0.0	0.0	0.0	0.05271481286241434	0.0
35	0.0	0.0	0.0	0.08785802143735723	0.0
36	0.0	0.0	0.0	0.10542962572482868	0.0
37	0.0	0.0	0.0	0.12300123001230012	0.0
38	0.0	0.0	0.0	0.158144438587243	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE