FastQCFastQC Report
Thu 2 Feb 2017
SRR4062624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4623022
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCA61610.13326780620987744No Hit
GCGCAAGACGGACCAGAGCGAAAGC60150.13010969880740347No Hit
GTATCAACGCAGAGTACTTTTTTTT57240.12381511487507522No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG57160.12364206789411773No Hit
GGGTAGGCACACGCTGAGCCAGTCA56260.12169528935834613No Hit
GAATAGGACCGCGGTTCTATTTTGT55610.12028928263806661No Hit
GTACATGGAAGCAGTGGTATCAACG52560.11369186648906278No Hit
GAACTACGACGGTATCTGATCGTCT49930.10800294699008571No Hit
GTATCTGATCGTCTTCGAACCTCCG49380.10681324899600306No Hit
GTCCTATTCCATTATTCCTAGCTGC46450.10047540331843542No Hit
GAATAACGCCGCCGCATCGCCAGTC46410.10038887982795669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA11800.012.88232510
TCGCGTA4650.012.6678939
CGTCGTA9500.012.5009410
CCGTCGT9400.012.4320569
AAGACGG10300.012.3581465
CAAGACG10550.011.9743514
ATCGCCA8300.011.90592916
CGCATCG8300.011.79081113
AGAATTT13100.011.75075717
CGTCTTA2200.011.66072615
CCGACCA7800.011.4498289
CGCGTAA5250.011.40085810
GCGTTAT4000.011.39234351
CGCAAGA10450.011.3578482
CGCCGGT13150.011.3433057
CGACCAT7400.011.29814710
AAGAATT14650.011.2854716
CATCGCC8700.011.24870415
ACGGTAT9050.011.2331339
GTCGCGT5350.011.1880018
Produced by FastQC (version 0.11.5)