##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062624_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4623022
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187158097019655	32.0	32.0	32.0	32.0	32.0
2	30.764335968983058	32.0	32.0	32.0	32.0	32.0
3	30.753133772670775	32.0	32.0	32.0	32.0	32.0
4	30.848400894479845	32.0	32.0	32.0	32.0	32.0
5	30.579680996542955	32.0	32.0	32.0	32.0	32.0
6	34.26167018024141	36.0	36.0	36.0	32.0	36.0
7	34.14743667670195	36.0	36.0	36.0	32.0	36.0
8	34.131370129754956	36.0	36.0	36.0	32.0	36.0
9	34.342203000548125	36.0	36.0	36.0	32.0	36.0
10	33.929018724115956	36.0	36.0	36.0	32.0	36.0
11	34.31142724391102	36.0	36.0	36.0	32.0	36.0
12	34.04287952772018	36.0	36.0	36.0	32.0	36.0
13	34.19773191648233	36.0	36.0	36.0	32.0	36.0
14	34.085778090608265	36.0	36.0	36.0	32.0	36.0
15	34.00301620887809	36.0	36.0	36.0	32.0	36.0
16	34.01397743726939	36.0	36.0	36.0	32.0	36.0
17	33.917944366260855	36.0	36.0	36.0	32.0	36.0
18	33.934523997506396	36.0	36.0	36.0	32.0	36.0
19	33.92796270491466	36.0	36.0	36.0	32.0	36.0
20	33.92639273617993	36.0	36.0	36.0	32.0	36.0
21	33.904156415435615	36.0	36.0	36.0	32.0	36.0
22	33.855575422310345	36.0	36.0	36.0	32.0	36.0
23	33.803600977888486	36.0	36.0	36.0	32.0	36.0
24	33.78057686076337	36.0	36.0	36.0	32.0	36.0
25	33.298462347788956	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	28.0
4	159.0
5	407.0
6	745.0
7	210.0
8	624.0
9	854.0
10	549.0
11	156.0
12	355.0
13	221.0
14	664.0
15	792.0
16	1296.0
17	1781.0
18	2477.0
19	3676.0
20	5658.0
21	8648.0
22	13449.0
23	20516.0
24	29390.0
25	41822.0
26	58945.0
27	76272.0
28	102547.0
29	136720.0
30	175473.0
31	240456.0
32	345136.0
33	492671.0
34	1059501.0
35	1800820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.12634596143966	16.934231091641657	11.015061788485978	25.924361158432706
2	17.11218725860499	19.216962453402857	36.813654031727914	26.85719625626424
3	19.49856126717513	22.833972698038295	27.69295697992004	29.974509054866534
4	12.747408416510417	14.68948700957756	35.14398575448466	37.41911881942736
5	15.194167604133465	35.95499466947615	33.11353442401889	15.737303302371505
6	35.68248686651322	34.34226095928771	16.311037495682765	13.664214678516306
7	31.36969062578733	29.810157323449427	20.045125189500688	18.775026861262553
8	28.599646857001332	32.75604815838265	18.93965307467355	19.704651909942474
9	27.000888781114107	14.570294358675683	18.340783144986073	40.088033715224135
10	16.087710989275855	26.35357647977537	30.953907682832465	26.60480484811631
11	38.11416637178567	20.9210811831564	21.42390922545121	19.54084321960672
12	24.871813864678238	23.44165641134267	28.071366658912904	23.615163065066184
13	29.349187363985873	18.95495240304165	25.17936160716316	26.516498625809316
14	23.569422068757312	19.517217915285954	24.57561872940844	32.33774128654829
15	25.448705788804897	26.9105238950287	21.834633044806914	25.806137271359486
16	26.368945224867517	25.63436442725955	23.170185066808028	24.826505281064907
17	24.47041987419237	25.841608688288925	24.675681306396545	25.01229013112216
18	25.422999045527288	24.672394080493216	25.617077329616222	24.287529544363274
19	25.7238309037964	25.081852707360735	24.717124363918316	24.477192024924545
20	25.6854029040345	24.30060707176233	24.597191253592793	25.416798770610384
21	26.85099555619856	24.218912773882455	24.005234320753317	24.924857349165666
22	25.83136204909581	24.24863743100505	24.628510402315285	25.291490117583855
23	24.39591144496501	24.03888849178758	25.20777423274613	26.35742583050128
24	24.721368498796846	25.095548436982217	24.763625100984832	25.41945796323611
25	25.107425343717782	24.43987212918764	24.647922099299137	25.804780427795443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	377.0
1	377.0
2	1019.0
3	1661.0
4	1661.0
5	1661.0
6	3839.5
7	6018.0
8	6018.0
9	6018.0
10	6962.5
11	7907.0
12	7907.0
13	7907.0
14	9901.5
15	11896.0
16	11896.0
17	11896.0
18	18971.5
19	26047.0
20	26047.0
21	26047.0
22	42384.0
23	58721.0
24	58721.0
25	58721.0
26	87366.5
27	116012.0
28	116012.0
29	116012.0
30	146118.5
31	176225.0
32	176225.0
33	176225.0
34	224128.0
35	272031.0
36	272031.0
37	272031.0
38	314145.0
39	356259.0
40	356259.0
41	356259.0
42	411561.0
43	466863.0
44	466863.0
45	466863.0
46	535872.0
47	604881.0
48	604881.0
49	604881.0
50	613942.0
51	623003.0
52	623003.0
53	623003.0
54	586152.5
55	549302.0
56	549302.0
57	549302.0
58	510541.0
59	471780.0
60	471780.0
61	471780.0
62	418204.5
63	364629.0
64	364629.0
65	364629.0
66	301954.5
67	239280.0
68	239280.0
69	239280.0
70	180850.0
71	122420.0
72	122420.0
73	122420.0
74	96039.0
75	69658.0
76	69658.0
77	69658.0
78	57657.5
79	45657.0
80	45657.0
81	45657.0
82	31796.5
83	17936.0
84	17936.0
85	17936.0
86	13328.0
87	8720.0
88	8720.0
89	8720.0
90	5935.0
91	3150.0
92	3150.0
93	3150.0
94	1877.5
95	605.0
96	605.0
97	605.0
98	1294.5
99	1984.0
100	1984.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008587456430014826
2	0.003309523510811759
3	0.004412698014415678
4	0.01416822156589348
5	0.032987080745019166
6	0.04347805396556625
7	0.06458978564237852
8	0.08500932939536088
9	0.09381309455157254
10	0.11488156448314543
11	0.11550885978911629
12	0.1277951954370972
13	0.11669855778319894
14	0.11972687994995482
15	0.10975504767228017
16	0.10845719531509908
17	0.1188832759177871
18	0.12574026253822715
19	0.12734094711208382
20	0.11780173228680287
21	0.12554558468465
22	0.14583534320191424
23	0.13480359816587503
24	0.12961218873715072
25	0.1268001752965917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4623022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.2923670187055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27558934464791	32.45549296765091
2	14.544867755570362	12.884532417365158
3	5.087212292655479	6.759740219051001
4	2.400241103714969	4.252494395965047
5	1.2488195613529658	2.7656587175792184
6	0.7364394057097773	1.9571186666840892
7	0.5129315154819492	1.5903265657431116
8	0.355937235025598	1.261224211950162
9	0.2733990185766547	1.0898540706015964
>10	1.3712043419588518	10.774014243911841
>50	0.09281453838875914	2.8411224541248203
>100	0.07580043184858808	7.037221160833695
>500	0.013278171164088715	4.149794869292638
>1k	0.011122305325695341	9.312048451417027
>5k	3.4297857832540473E-4	0.8693565878298287
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCA	6161	0.13326780620987744	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	6015	0.13010969880740347	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5724	0.12381511487507522	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5716	0.12364206789411773	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	5626	0.12169528935834613	No Hit
GAATAGGACCGCGGTTCTATTTTGT	5561	0.12028928263806661	No Hit
GTACATGGAAGCAGTGGTATCAACG	5256	0.11369186648906278	No Hit
GAACTACGACGGTATCTGATCGTCT	4993	0.10800294699008571	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4938	0.10681324899600306	No Hit
GTCCTATTCCATTATTCCTAGCTGC	4645	0.10047540331843542	No Hit
GAATAACGCCGCCGCATCGCCAGTC	4641	0.10038887982795669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.48926178590541E-5
2	0.0	0.0	0.0	0.0	6.48926178590541E-5
3	0.0	0.0	0.0	0.0	6.48926178590541E-5
4	0.0	0.0	0.0	0.0	6.48926178590541E-5
5	0.0	0.0	0.0	0.0	6.48926178590541E-5
6	0.0	0.0	0.0	0.0	8.65234904787388E-5
7	0.0	0.0	0.0	2.16308726196847E-5	8.65234904787388E-5
8	0.0	0.0	0.0	2.16308726196847E-5	8.65234904787388E-5
9	0.0	0.0	0.0	4.32617452393694E-5	8.65234904787388E-5
10	2.16308726196847E-5	0.0	0.0	4.32617452393694E-5	8.65234904787388E-5
11	2.16308726196847E-5	0.0	0.0	4.32617452393694E-5	8.65234904787388E-5
12	2.16308726196847E-5	0.0	0.0	8.65234904787388E-5	2.379395988165317E-4
13	2.16308726196847E-5	0.0	0.0	1.081543630984235E-4	2.379395988165317E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	1180	0.0	12.882325	10
TCGCGTA	465	0.0	12.667893	9
CGTCGTA	950	0.0	12.50094	10
CCGTCGT	940	0.0	12.432056	9
AAGACGG	1030	0.0	12.358146	5
CAAGACG	1055	0.0	11.974351	4
ATCGCCA	830	0.0	11.905929	16
CGCATCG	830	0.0	11.790811	13
AGAATTT	1310	0.0	11.750757	17
CGTCTTA	220	0.0	11.660726	15
CCGACCA	780	0.0	11.449828	9
CGCGTAA	525	0.0	11.400858	10
GCGTTAT	400	0.0	11.3923435	1
CGCAAGA	1045	0.0	11.357848	2
CGCCGGT	1315	0.0	11.343305	7
CGACCAT	740	0.0	11.298147	10
AAGAATT	1465	0.0	11.28547	16
CATCGCC	870	0.0	11.248704	15
ACGGTAT	905	0.0	11.233133	9
GTCGCGT	535	0.0	11.188001	8
>>END_MODULE