FastQCFastQC Report
Thu 2 Feb 2017
SRR4062624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4623022
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC64240.1389567257088545No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT59310.12829270550734995No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT57620.12463708803462324No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC54930.11881838329992807No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA53960.11672018865581864No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA48730.10540724227572354No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA47810.10341720199471255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA15850.018.73803736
CTAGCGG16750.017.73121629
TCTAGCG16800.017.67844628
CAATACG17100.017.62560538
TAGCGGC17000.017.47046330
ATACGAA17650.017.20101240
GTATCAA77350.016.8124621
AATACGA17800.016.80886839
CGGTCCA19400.016.78338410
ATCGTTT16000.016.77488129
TACGAAT17850.016.76178641
CGAATGC17900.016.71496643
CGCCGGT18050.016.697947
TACGACG16000.016.5000615
TACCGTC14000.016.3427417
CGTCGTA14150.016.16949510
GCAATAC18950.016.02099637
CGACGGT16700.015.9400067
ATACCGT14650.015.9181486
GTATTAG12700.015.7667371
Produced by FastQC (version 0.11.5)