##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062624_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4623022 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.310122685983323 32.0 32.0 32.0 32.0 32.0 2 31.401513988036395 32.0 32.0 32.0 32.0 32.0 3 31.50149642376783 32.0 32.0 32.0 32.0 32.0 4 31.60698348396352 32.0 32.0 32.0 32.0 32.0 5 31.535397625189756 32.0 32.0 32.0 32.0 32.0 6 35.14742477972201 36.0 36.0 36.0 36.0 36.0 7 35.15734772622756 36.0 36.0 36.0 36.0 36.0 8 35.09760217450836 36.0 36.0 36.0 36.0 36.0 9 35.242762418175815 36.0 36.0 36.0 36.0 36.0 10 35.108445298335155 36.0 36.0 36.0 36.0 36.0 11 35.24249051810699 36.0 36.0 36.0 36.0 36.0 12 35.15352793043165 36.0 36.0 36.0 36.0 36.0 13 35.20085801019333 36.0 36.0 36.0 36.0 36.0 14 35.16131547719219 36.0 36.0 36.0 36.0 36.0 15 35.11890447417296 36.0 36.0 36.0 36.0 36.0 16 35.13328900446504 36.0 36.0 36.0 36.0 36.0 17 35.1000845334502 36.0 36.0 36.0 36.0 36.0 18 35.119482451089354 36.0 36.0 36.0 36.0 36.0 19 35.10445440233683 36.0 36.0 36.0 36.0 36.0 20 35.101681757084435 36.0 36.0 36.0 36.0 36.0 21 35.10045809862034 36.0 36.0 36.0 36.0 36.0 22 35.08656805007634 36.0 36.0 36.0 36.0 36.0 23 35.043222809668656 36.0 36.0 36.0 36.0 36.0 24 35.01368779988501 36.0 36.0 36.0 36.0 36.0 25 34.98813935992518 36.0 36.0 36.0 36.0 36.0 26 34.945572614623075 36.0 36.0 36.0 32.0 36.0 27 34.92797546712951 36.0 36.0 36.0 32.0 36.0 28 34.91625737450525 36.0 36.0 36.0 32.0 36.0 29 34.89092459434543 36.0 36.0 36.0 32.0 36.0 30 34.87829151580936 36.0 36.0 36.0 32.0 36.0 31 34.880761545153796 36.0 36.0 36.0 32.0 36.0 32 34.84599532513581 36.0 36.0 36.0 32.0 36.0 33 34.83072241490523 36.0 36.0 36.0 32.0 36.0 34 34.81950031819014 36.0 36.0 36.0 32.0 36.0 35 34.79357528473799 36.0 36.0 36.0 32.0 36.0 36 34.76869869968172 36.0 36.0 36.0 32.0 36.0 37 34.76345083367546 36.0 36.0 36.0 32.0 36.0 38 34.72312439785058 36.0 36.0 36.0 32.0 36.0 39 34.71563665498455 36.0 36.0 36.0 32.0 36.0 40 34.70653265331638 36.0 36.0 36.0 32.0 36.0 41 34.6776727430672 36.0 36.0 36.0 32.0 36.0 42 34.647020931330196 36.0 36.0 36.0 32.0 36.0 43 34.64676049562386 36.0 36.0 36.0 32.0 36.0 44 34.62011969659673 36.0 36.0 36.0 32.0 36.0 45 34.57730203317224 36.0 36.0 36.0 32.0 36.0 46 34.59443541475684 36.0 36.0 36.0 32.0 36.0 47 34.5689142296965 36.0 36.0 36.0 32.0 36.0 48 34.533434839808244 36.0 36.0 36.0 32.0 36.0 49 34.52849607897172 36.0 36.0 36.0 32.0 36.0 50 34.01995274952185 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 11.0 20 44.0 21 107.0 22 331.0 23 1005.0 24 2750.0 25 6305.0 26 13250.0 27 24640.0 28 41565.0 29 64443.0 30 95191.0 31 139342.0 32 210558.0 33 354636.0 34 812497.0 35 2856345.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69119547376161 17.40067105222451 11.52430189951178 26.383831574502093 2 16.48145243892019 19.61519367402541 36.63285271299384 27.270501174060563 3 18.872436835333623 23.073240816128763 28.10716254134765 29.947159807189973 4 12.521960781429604 15.15439927285533 35.3723350178044 36.95130492791067 5 14.924977644493149 36.13660069106312 33.061317899849925 15.877103764593809 6 35.89284791106379 34.50675632852181 16.078480657253007 13.521915103161389 7 31.416050853349294 29.819756310757107 20.024966369378973 18.73922646651463 8 28.658879118221613 32.795459938425545 18.847048282930146 19.698612660422697 9 27.108501754912695 14.29852594255446 18.282651477756325 40.31032082477652 10 16.0685153564054 26.392454978583274 31.005454873457232 26.5335747915541 11 38.26237469776263 20.803145648019843 21.45447285347117 19.480006800746352 12 24.915261056512385 23.404106664428593 28.023509297597975 23.657122981461047 13 29.559171468359875 18.79608187025716 25.16561677621262 26.479129885170348 14 23.593917571666324 19.371982222883645 24.55476093343272 32.47933927201731 15 25.482682107071952 26.89483199517545 21.838593889451534 25.783892008301063 16 26.507314912193795 25.573834604291307 23.149186830605608 24.769663652909287 17 24.574336873153534 25.8086593574506 24.62333079963712 24.993672969758745 18 25.582054335886784 24.5740773026821 25.59390805408237 24.249960307348744 19 25.80965437759111 25.043575392892354 24.691143585299834 24.455626644216704 20 25.76007641754679 24.211197783614267 24.655301229369016 25.373424569469925 21 26.925374787314443 24.197808273462684 24.01954825220386 24.857268687019012 22 25.840262062347964 24.204427320484307 24.66391031667165 25.29140030049608 23 24.428328482970663 23.896100862163323 25.27031019969189 26.40526045517413 24 24.839206908381573 25.00089768121372 24.768409062297344 25.39148634810736 25 25.20191338046845 24.308882804364764 24.75177492125281 25.737428893913982 26 24.91123771420512 25.147165641868025 25.186317521309654 24.755279122617196 27 25.85010410938992 24.473926362452957 24.41898394599896 25.256985582158165 28 24.936178110335618 24.38599686525394 25.237301488074248 25.44052353633619 29 24.811065143103363 24.70436004847046 25.248744219690067 25.235830588736114 30 24.834556270768342 24.793262934937363 25.455340684080674 24.916840110213624 31 25.34562889815363 24.69464778666422 24.27834001222577 25.68138330295638 32 25.077903587739797 24.6082540814212 24.39555771095184 25.918284619887167 33 24.627267618453903 24.14929022617673 25.09817171538444 26.125270439984927 34 25.40398899248154 24.47379657721724 25.258218541897488 24.863995888403736 35 26.14488964145098 24.164842823590284 25.018656627634478 24.671610907324258 36 24.762222632728115 24.90528922423471 24.758329075656572 25.574159067380602 37 26.0034886271361 24.5959461149006 24.55657792673277 24.843987331230526 38 24.937908580145198 24.271785857822003 25.098301500620156 25.692004061412643 39 25.65858436321523 24.270877361171976 24.525451101033045 25.545087174579745 40 25.834724558957323 24.419156992979918 24.90394811013229 24.84217033793047 41 24.699752672602465 24.901914808106042 25.42959994566325 24.968732573628248 42 26.145452044139095 24.87738539855532 24.776650424765446 24.200512132540144 43 25.2761505352992 23.781457237278993 25.05770035271301 25.884691874708793 44 24.768430693169964 24.524218141293723 24.949481096996724 25.757870068539585 45 24.90663033833713 24.742452015153724 24.924843533082903 25.42607411342624 46 24.70667938852865 24.46036816249489 25.23460349343599 25.598348955540466 47 25.02528650103179 24.236040510315767 25.514706836656558 25.22396615199588 48 25.860357143011647 25.015736459830823 24.346001381780145 24.77790501537739 49 24.697719370576216 25.0583492788916 24.74623741786217 25.497693932670018 50 24.70709062934443 25.223441748105184 24.65898351660831 25.41048410594207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27.0 1 36.5 2 46.0 3 184.5 4 323.0 5 414.5 6 506.0 7 479.0 8 452.0 9 577.5 10 703.0 11 1077.0 12 1451.0 13 2646.0 14 3841.0 15 5116.0 16 6391.0 17 7152.5 18 7914.0 19 8714.0 20 9514.0 21 11401.0 22 13288.0 23 15355.0 24 17422.0 25 21569.5 26 25717.0 27 32040.0 28 38363.0 29 44672.0 30 50981.0 31 57763.5 32 64546.0 33 74261.0 34 83976.0 35 98301.5 36 112627.0 37 135366.5 38 158106.0 39 174997.0 40 191888.0 41 211013.5 42 230139.0 43 240538.5 44 250938.0 45 277812.0 46 304686.0 47 328449.5 48 352213.0 49 363032.5 50 373852.0 51 356075.5 52 338299.0 53 333343.5 54 328388.0 55 335009.5 56 341631.0 57 332079.0 58 322527.0 59 295344.5 60 268162.0 61 235889.5 62 203617.0 63 177050.5 64 150484.0 65 129167.0 66 107850.0 67 94186.0 68 80522.0 69 75243.0 70 69964.0 71 53849.0 72 37734.0 73 34295.0 74 30856.0 75 21980.0 76 13104.0 77 11908.5 78 10713.0 79 9265.5 80 7818.0 81 6188.0 82 4558.0 83 3927.5 84 3297.0 85 2709.5 86 2122.0 87 1557.0 88 992.0 89 638.5 90 285.0 91 190.5 92 96.0 93 64.0 94 32.0 95 28.5 96 25.0 97 23.0 98 21.0 99 18.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015768906139750145 2 0.003828664453684192 3 5.624026881118022E-4 4 1.730469809574776E-4 5 0.0 6 4.975100702527481E-4 7 6.489261785905411E-5 8 2.16308726196847E-5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.9467785357716227E-4 47 4.32617452393694E-5 48 0.0 49 0.0 50 1.0815436309842349E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4623022.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.394268971541976 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33563031452397 34.02352958009593 2 14.928120703562236 13.851584943214219 3 5.035556620786234 7.008629048585567 4 2.2280493148502845 4.134748767800957 5 1.2129229073758943 2.8136335803270964 6 0.7522950750032923 2.094130803340145 7 0.4731911662623707 1.5367350769773847 8 0.33634439178206615 1.2483561743525495 9 0.23285930214401923 0.972300338657567 >10 1.2944000360074273 10.59421598328812 >50 0.08191918560362133 2.632243803083593 >100 0.06697034333189991 6.4136488335741575 >500 0.012161610393398295 3.967379580164496 >1k 0.009344248266270491 8.076970593469605 >5k 2.3478010719272204E-4 0.6318928930687702 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 6424 0.1389567257088545 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 5931 0.12829270550734995 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 5762 0.12463708803462324 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC 5493 0.11881838329992807 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 5396 0.11672018865581864 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA 4873 0.10540724227572354 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 4781 0.10341720199471255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.16308726196847E-5 2 0.0 0.0 0.0 0.0 2.16308726196847E-5 3 0.0 0.0 0.0 0.0 2.16308726196847E-5 4 0.0 0.0 0.0 2.16308726196847E-5 2.16308726196847E-5 5 0.0 0.0 0.0 2.16308726196847E-5 4.32617452393694E-5 6 0.0 0.0 0.0 2.16308726196847E-5 8.65234904787388E-5 7 0.0 0.0 0.0 2.16308726196847E-5 8.65234904787388E-5 8 0.0 0.0 0.0 2.16308726196847E-5 8.65234904787388E-5 9 0.0 0.0 0.0 4.32617452393694E-5 8.65234904787388E-5 10 0.0 0.0 0.0 4.32617452393694E-5 8.65234904787388E-5 11 0.0 0.0 0.0 4.32617452393694E-5 8.65234904787388E-5 12 0.0 0.0 0.0 6.48926178590541E-5 1.946778535771623E-4 13 0.0 0.0 0.0 8.65234904787388E-5 1.946778535771623E-4 14 0.0 0.0 0.0 8.65234904787388E-5 1.946778535771623E-4 15 0.0 0.0 0.0 1.081543630984235E-4 3.028322166755858E-4 16 0.0 0.0 0.0 1.514161083377929E-4 3.028322166755858E-4 17 0.0 0.0 0.0 1.514161083377929E-4 3.028322166755858E-4 18 0.0 0.0 0.0 2.379395988165317E-4 3.028322166755858E-4 19 0.0 0.0 0.0 4.109865797740093E-4 3.677248345346399E-4 20 0.0 0.0 0.0 5.840335607314869E-4 4.758791976330634E-4 21 0.0 0.0 0.0 6.921879238299104E-4 4.975100702527481E-4 22 0.0 0.0 0.0 0.001232959739322028 5.407718154921175E-4 23 0.0 0.0 0.0 0.0020549328988700463 5.407718154921175E-4 24 0.0 0.0 0.0 0.0031364765298542816 5.407718154921175E-4 25 0.0 0.0 0.0 0.003936818816782615 5.407718154921175E-4 26 0.0 0.0 0.0 0.005061624193006219 6.056644333511716E-4 27 0.0 0.0 0.0 0.006640677894243203 6.056644333511716E-4 28 0.0 0.0 0.0 0.010599127583645503 6.056644333511716E-4 29 0.0 0.0 0.0 0.018407872599351678 6.056644333511716E-4 30 0.0 0.0 0.0 0.032792402891442 6.056644333511716E-4 31 0.0 0.0 0.0 0.06504403396739189 6.272953059708563E-4 32 0.0 0.0 0.0 0.1013622690958425 6.272953059708563E-4 33 0.0 0.0 0.0 0.13837269214812303 6.272953059708563E-4 34 0.0 0.0 0.0 0.18057452462912787 6.705570512102257E-4 35 0.0 0.0 0.0 0.22876810882578538 6.705570512102257E-4 36 0.0 0.0 0.0 0.30049608243265985 6.705570512102257E-4 37 0.0 2.16308726196847E-5 0.0 0.40144736494872835 6.705570512102257E-4 38 0.0 2.16308726196847E-5 0.0 0.5361211778788852 7.354496690692798E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAATA 1585 0.0 18.738037 36 CTAGCGG 1675 0.0 17.731216 29 TCTAGCG 1680 0.0 17.678446 28 CAATACG 1710 0.0 17.625605 38 TAGCGGC 1700 0.0 17.470463 30 ATACGAA 1765 0.0 17.201012 40 GTATCAA 7735 0.0 16.812462 1 AATACGA 1780 0.0 16.808868 39 CGGTCCA 1940 0.0 16.783384 10 ATCGTTT 1600 0.0 16.774881 29 TACGAAT 1785 0.0 16.761786 41 CGAATGC 1790 0.0 16.714966 43 CGCCGGT 1805 0.0 16.69794 7 TACGACG 1600 0.0 16.500061 5 TACCGTC 1400 0.0 16.342741 7 CGTCGTA 1415 0.0 16.169495 10 GCAATAC 1895 0.0 16.020996 37 CGACGGT 1670 0.0 15.940006 7 ATACCGT 1465 0.0 15.918148 6 GTATTAG 1270 0.0 15.766737 1 >>END_MODULE