FastQCFastQC Report
Thu 2 Feb 2017
SRR4062623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653471
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCAC15140.23168587435402643No Hit
GTACATGGAAGCAGTGGTATCAACG14200.2173011503188359No Hit
GTATCAACGCAGAGTACTTTTTTTT11440.17506515208785087No Hit
TATCAACGCAGAGTACTTTTTTTTT9130.1397154579162656No Hit
GCGCAAGACGGACCAGAGCGAAAGC7610.11645505309340429No Hit
GAACTACGACGGTATCTGATCGTCT7060.10803845924302685No Hit
GTATCTGATCGTCTTCGAACCTCCG6950.10635514047295136No Hit
GAGTACATGGAAGCAGTGGTATCAA6750.10329456089099592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA250.006024339219.00222213
ACGCCCC653.3608048E-614.61709317
TCCGATA653.3634788E-614.6159738
ATTCCGA602.5627842E-514.2505756
TGCGCAC400.0052733514.25057510
ACGAACG602.5627842E-514.25057515
AATTCCG602.5646368E-514.2494835
CGTCTAT400.005289457314.2440261
CGAACGA707.2440944E-613.57197716
GCCTTGG1051.373337E-913.5657411
TTAGGCC500.001498331913.2995184
AACGAAC655.440981E-513.15437614
TAGACTG655.4448952E-513.1533685
AACCTCC2200.012.52419218
CGAACCT2000.012.35049916
AGACTGG853.9316474E-612.2946146
CCGATAA853.9316474E-612.2946149
TAACGAA701.08784276E-412.21571413
CACGCCC701.08862136E-412.21477916
ACCGTCG701.08862136E-412.2147798
Produced by FastQC (version 0.11.5)