##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062623_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653471
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.42505635292155	32.0	21.0	32.0	14.0	32.0
2	24.46980355669953	32.0	14.0	32.0	14.0	32.0
3	25.142684220110763	32.0	14.0	32.0	14.0	32.0
4	24.229141002431632	32.0	14.0	32.0	14.0	32.0
5	24.57386785335539	32.0	14.0	32.0	14.0	32.0
6	27.88528947726831	32.0	21.0	36.0	14.0	36.0
7	27.800471635313578	32.0	21.0	36.0	14.0	36.0
8	27.319631628641517	32.0	21.0	36.0	14.0	36.0
9	28.019853979748145	32.0	21.0	36.0	14.0	36.0
10	25.804406010366183	32.0	14.0	36.0	14.0	36.0
11	28.714458637032095	32.0	21.0	36.0	14.0	36.0
12	27.073554909093136	32.0	14.0	36.0	14.0	36.0
13	27.76952917574001	32.0	21.0	36.0	14.0	36.0
14	26.908208627467783	32.0	14.0	36.0	14.0	36.0
15	26.802029470320793	32.0	14.0	36.0	14.0	36.0
16	26.961298971186174	32.0	14.0	36.0	14.0	36.0
17	26.56280538845641	32.0	14.0	36.0	14.0	36.0
18	26.86411485743055	32.0	14.0	36.0	14.0	36.0
19	26.72276351972773	32.0	14.0	36.0	14.0	36.0
20	26.607824983817185	32.0	14.0	36.0	14.0	36.0
21	26.611050834696567	32.0	14.0	36.0	14.0	36.0
22	26.46951739250862	32.0	14.0	36.0	14.0	36.0
23	26.34698555865524	32.0	14.0	36.0	14.0	36.0
24	26.26274157537213	32.0	14.0	36.0	14.0	36.0
25	25.678755445918792	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
2	3.0
3	17.0
4	82.0
5	94.0
6	107.0
7	96.0
8	96.0
9	77.0
10	49.0
11	38.0
12	27.0
13	22.0
14	1010.0
15	2332.0
16	5066.0
17	10514.0
18	18006.0
19	26026.0
20	32509.0
21	37016.0
22	39627.0
23	41733.0
24	43369.0
25	43455.0
26	43751.0
27	42834.0
28	41499.0
29	38830.0
30	36156.0
31	34741.0
32	32601.0
33	29438.0
34	31090.0
35	21160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.407320119002435	19.42742320547509	12.052975673061619	26.11228100246085
2	16.0801159379481	20.930503035413413	37.79919902426035	25.190182002378137
3	17.966148383937316	25.045604799216452	29.851707884427032	27.136538932419196
4	11.98135804642122	16.16033916723424	37.88892885293825	33.96937393340629
5	13.575756462422579	36.99218355331441	34.91361494585434	14.518445038408665
6	31.893166351534454	37.21555809022223	17.611323325652783	13.279952232590539
7	27.902731559256782	31.73307074917649	22.394283587826592	17.96991410374014
8	26.63649243729657	33.376373731571896	21.02040972621099	18.966724104920544
9	26.49141134554055	14.722182225912094	19.937564908905426	38.84884151964193
10	14.756346243119062	27.203700270717885	33.23778284043885	24.802170645724207
11	35.80638439995343	22.17518798374791	23.432243119519054	18.58618449677961
12	22.993493737377452	24.939549679590318	30.142874217363726	21.92408236566851
13	27.938242891681455	20.640859463815676	26.502636796254247	24.91826084824862
14	22.331465623568384	20.645930338562614	26.430927680255444	30.59167635761356
15	24.049116410181774	27.815575999448473	23.794801872122683	24.34050571824707
16	24.62894646533974	26.717080105892734	24.796855083831748	23.857118344935778
17	22.424330639292144	26.870494503389896	26.958133833837667	23.747041023480293
18	23.487690072995385	25.81912957140493	27.67986835089268	23.013312004707007
19	23.805094809423483	26.856157824171618	26.713962842367362	22.62478452403754
20	23.9541535864048	25.52638957194795	26.739502467439145	23.7799543742081
21	25.016930766189947	25.75498959638892	25.558102405854218	23.669977231566914
22	23.91951019532417	25.96593129554563	26.234281729641918	23.88027677948828
23	22.93338154983359	25.389205224543833	26.811183367759096	24.866229857863477
24	22.834636017204314	26.62746101525067	26.442822623029695	24.09508034451532
25	23.445661550530843	25.632775139778225	26.551810260158344	24.36975304953259
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	67.0
2	213.0
3	359.0
4	359.0
5	359.0
6	814.5
7	1270.0
8	1270.0
9	1270.0
10	1645.0
11	2020.0
12	2020.0
13	2020.0
14	2716.0
15	3412.0
16	3412.0
17	3412.0
18	5574.0
19	7736.0
20	7736.0
21	7736.0
22	11768.0
23	15800.0
24	15800.0
25	15800.0
26	21750.0
27	27700.0
28	27700.0
29	27700.0
30	32535.5
31	37371.0
32	37371.0
33	37371.0
34	44056.0
35	50741.0
36	50741.0
37	50741.0
38	55549.5
39	60358.0
40	60358.0
41	60358.0
42	66178.0
43	71998.0
44	71998.0
45	71998.0
46	80792.0
47	89586.0
48	89586.0
49	89586.0
50	85624.5
51	81663.0
52	81663.0
53	81663.0
54	72727.5
55	63792.0
56	63792.0
57	63792.0
58	56982.0
59	50172.0
60	50172.0
61	50172.0
62	43930.5
63	37689.0
64	37689.0
65	37689.0
66	30821.5
67	23954.0
68	23954.0
69	23954.0
70	18324.0
71	12694.0
72	12694.0
73	12694.0
74	9867.5
75	7041.0
76	7041.0
77	7041.0
78	5823.5
79	4606.0
80	4606.0
81	4606.0
82	3268.0
83	1930.0
84	1930.0
85	1930.0
86	1406.5
87	883.0
88	883.0
89	883.0
90	574.5
91	266.0
92	266.0
93	266.0
94	157.5
95	49.0
96	49.0
97	49.0
98	181.5
99	314.0
100	314.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005968130184813098
2	0.002754521623759891
3	0.004743898352030924
4	0.016221071784363807
5	0.03565575212978082
6	0.04682686760391815
7	0.07253573609234382
8	0.09120527154227195
9	0.09686734376888952
10	0.11660808207250206
11	0.11553687921881768
12	0.13298218283596364
13	0.12012774859175082
14	0.12242318327821741
15	0.11262932861596002
16	0.11247629963686222
17	0.1218110673618263
18	0.12655496571385724
19	0.1294625163167149
20	0.11783231390528423
21	0.12441256000648843
22	0.14782599380844752
23	0.13145189304498592
24	0.12915645835851935
25	0.12701405265115054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	653471.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.91368511662141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.16263933155848	52.72732835274508
2	9.744689646168384	12.066592926241402
3	2.257765558441897	4.193597575575735
4	0.866727123931613	2.1464908093254715
5	0.43918765878374716	1.3595863206521543
6	0.2660551386633694	0.9883472447317739
7	0.18373486555863233	0.796299182779935
8	0.12145996054815983	0.6016027001324822
9	0.09815536276291865	0.5469444200349982
>10	0.6664027246963782	8.26900399011676
>50	0.10460980179758356	4.59484534872371
>100	0.0843785706529974	9.747540960930953
>500	0.003454093535492674	1.3388891826367815
>1k	7.401629004627159E-4	0.6229309853728232
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCAC	1514	0.23168587435402643	No Hit
GTACATGGAAGCAGTGGTATCAACG	1420	0.2173011503188359	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1144	0.17506515208785087	No Hit
TATCAACGCAGAGTACTTTTTTTTT	913	0.1397154579162656	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	761	0.11645505309340429	No Hit
GAACTACGACGGTATCTGATCGTCT	706	0.10803845924302685	No Hit
GTATCTGATCGTCTTCGAACCTCCG	695	0.10635514047295136	No Hit
GAGTACATGGAAGCAGTGGTATCAA	675	0.10329456089099592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.5302897909777176E-4
6	0.0	0.0	0.0	0.0	1.5302897909777176E-4
7	0.0	0.0	0.0	0.0	1.5302897909777176E-4
8	0.0	0.0	0.0	1.5302897909777176E-4	1.5302897909777176E-4
9	0.0	0.0	0.0	3.060579581955435E-4	1.5302897909777176E-4
10	0.0	0.0	0.0	3.060579581955435E-4	1.5302897909777176E-4
11	0.0	0.0	0.0	3.060579581955435E-4	1.5302897909777176E-4
12	0.0	0.0	0.0	3.060579581955435E-4	1.5302897909777176E-4
13	0.0	0.0	0.0	3.060579581955435E-4	3.060579581955435E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	25	0.0060243392	19.002222	13
ACGCCCC	65	3.3608048E-6	14.617093	17
TCCGATA	65	3.3634788E-6	14.615973	8
ATTCCGA	60	2.5627842E-5	14.250575	6
TGCGCAC	40	0.00527335	14.250575	10
ACGAACG	60	2.5627842E-5	14.250575	15
AATTCCG	60	2.5646368E-5	14.249483	5
CGTCTAT	40	0.0052894573	14.244026	1
CGAACGA	70	7.2440944E-6	13.571977	16
GCCTTGG	105	1.373337E-9	13.565741	1
TTAGGCC	50	0.0014983319	13.299518	4
AACGAAC	65	5.440981E-5	13.154376	14
TAGACTG	65	5.4448952E-5	13.153368	5
AACCTCC	220	0.0	12.524192	18
CGAACCT	200	0.0	12.350499	16
AGACTGG	85	3.9316474E-6	12.294614	6
CCGATAA	85	3.9316474E-6	12.294614	9
TAACGAA	70	1.08784276E-4	12.215714	13
CACGCCC	70	1.08862136E-4	12.214779	16
ACCGTCG	70	1.08862136E-4	12.214779	8
>>END_MODULE