FastQCFastQC Report
Thu 2 Feb 2017
SRR4062623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653471
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11030.16879096394484225No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG8620.13191097998227924No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC7930.121351980424533No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7920.12119895144543523No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6670.10207032905821375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC300.005743107829.3333321
TAGTACG450.001397527324.4444452
GTATAGA752.0618536E-623.4666671
GTATTAT852.3492248E-723.2941171
CATCCCG703.2124393E-522.015
TAATACC550.004481444620.0000024
TAACGGC808.9805195E-519.25000236
ATCCCGA808.9805195E-519.25000216
TTAACGG808.9805195E-519.25000235
GCACCGG708.11787E-418.8571436
GTAATAC708.11787E-418.8571433
TATAGGG708.11787E-418.8571434
GTAAACG1404.0708983E-918.85714327
CGGTCCA2100.018.85714110
TATAGAC600.00740855218.3333323
CGCACTG600.00740855218.3333325
ATACGAA2050.018.24390440
ATTTCGG851.4295593E-418.11764522
TAGCGGC2200.018.00000230
CTAGCGG2200.018.00000229
Produced by FastQC (version 0.11.5)