##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653471
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05396873005841	32.0	32.0	32.0	21.0	32.0
2	29.461823095439584	32.0	32.0	32.0	14.0	32.0
3	30.675267915485154	32.0	32.0	32.0	32.0	32.0
4	31.154735252214714	32.0	32.0	32.0	32.0	32.0
5	30.834491507656804	32.0	32.0	32.0	32.0	32.0
6	34.480175860902776	36.0	36.0	36.0	32.0	36.0
7	34.507981226404844	36.0	36.0	36.0	32.0	36.0
8	34.37717511565165	36.0	36.0	36.0	32.0	36.0
9	34.737870540544264	36.0	36.0	36.0	32.0	36.0
10	34.30044026437287	36.0	36.0	36.0	32.0	36.0
11	34.77088348220502	36.0	36.0	36.0	32.0	36.0
12	34.517404750937686	36.0	36.0	36.0	32.0	36.0
13	34.67993070847827	36.0	36.0	36.0	32.0	36.0
14	34.585719947786515	36.0	36.0	36.0	32.0	36.0
15	34.49081290523986	36.0	36.0	36.0	32.0	36.0
16	34.53676750766292	36.0	36.0	36.0	32.0	36.0
17	34.484423945362536	36.0	36.0	36.0	32.0	36.0
18	34.52139574671256	36.0	36.0	36.0	32.0	36.0
19	34.5139753715161	36.0	36.0	36.0	32.0	36.0
20	34.51843463596701	36.0	36.0	36.0	32.0	36.0
21	34.5249674430847	36.0	36.0	36.0	32.0	36.0
22	34.49074251190948	36.0	36.0	36.0	32.0	36.0
23	34.42439373744206	36.0	36.0	36.0	32.0	36.0
24	34.397348925966114	36.0	36.0	36.0	32.0	36.0
25	34.36510878064979	36.0	36.0	36.0	32.0	36.0
26	34.29188900502088	36.0	36.0	36.0	32.0	36.0
27	34.279028449617506	36.0	36.0	36.0	32.0	36.0
28	34.26069098705222	36.0	36.0	36.0	32.0	36.0
29	34.210648980597455	36.0	36.0	36.0	32.0	36.0
30	34.20298834990382	36.0	36.0	36.0	32.0	36.0
31	34.191384162418835	36.0	36.0	36.0	32.0	36.0
32	34.15367170080998	36.0	36.0	36.0	32.0	36.0
33	34.114791628090614	36.0	36.0	36.0	32.0	36.0
34	34.088799655990854	36.0	36.0	36.0	32.0	36.0
35	34.07916342117707	36.0	36.0	36.0	32.0	36.0
36	34.00805238488012	36.0	36.0	36.0	32.0	36.0
37	34.037775203490284	36.0	36.0	36.0	32.0	36.0
38	33.957794607564836	36.0	36.0	36.0	32.0	36.0
39	33.94343742874588	36.0	36.0	36.0	32.0	36.0
40	33.949892191084224	36.0	36.0	36.0	32.0	36.0
41	33.89058121936551	36.0	36.0	36.0	32.0	36.0
42	33.84440319463297	36.0	36.0	36.0	32.0	36.0
43	33.8390548318135	36.0	36.0	36.0	32.0	36.0
44	33.80312209723155	36.0	36.0	36.0	32.0	36.0
45	33.72389593417306	36.0	36.0	36.0	32.0	36.0
46	33.745479141385005	36.0	36.0	36.0	32.0	36.0
47	33.73740380215801	36.0	36.0	36.0	32.0	36.0
48	33.679730240515646	36.0	36.0	36.0	27.0	36.0
49	33.65440241418517	36.0	36.0	36.0	27.0	36.0
50	32.92152214864929	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	14.0
22	55.0
23	207.0
24	497.0
25	1146.0
26	2454.0
27	4747.0
28	8365.0
29	14412.0
30	23620.0
31	38440.0
32	62222.0
33	105230.0
34	183900.0
35	208158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.191961703156615	19.206424026127067	12.314400188545841	24.28721408217048
2	17.06631040757081	21.711910653646683	36.946884033685464	24.274894905097042
3	19.3685373553676	25.09476377537045	30.19065997211795	25.346038897144002
4	13.93589003949678	17.51171819407441	38.32105785872671	30.2313339077021
5	16.341046503976457	35.424678371343184	33.335373719721304	14.898901404959059
6	32.45688401915926	37.9948582184339	16.693038701088035	12.855219061318806
7	28.553371151895035	31.826048898879982	21.588869284176347	18.03171066504864
8	26.993741105176976	33.76528379267602	19.985156166312148	19.25581893583485
9	26.75053674914419	14.580295070477497	19.071848635976192	39.59731954440212
10	14.924610273447483	27.071285489333114	32.58553172214222	25.41857251507718
11	36.47812986345224	22.148037173799604	22.705215686694586	18.668617276053567
12	23.26361843142236	24.633686881284707	29.593509122822585	22.509185564470343
13	28.656512683806934	20.40794465247884	25.796401064469578	25.139141599244645
14	22.79580884232047	20.50557714114322	25.66265373673813	31.035960279798186
15	24.591909969991015	27.60198998884419	23.112731858031957	24.69336818313284
16	25.118788745024645	27.048637200426644	24.10420661360642	23.728367440942293
17	23.3018756761968	26.699731128083727	26.064201777890677	23.934191417828792
18	24.13420029350958	25.778802731873334	26.791395486563292	23.295601488053794
19	24.44668546882723	26.939680567309033	25.902297118005237	22.7113368458585
20	24.29518677952044	25.657909838386097	25.92754689955637	24.1193564825371
21	25.465246353702	25.908724335127342	24.96729005571785	23.658739255452804
22	24.251573520477574	26.130310296860916	25.619958651569846	23.998157531091664
23	23.521931348139397	25.480396222632677	26.03206569228015	24.965606736947777
24	23.469901495246155	26.82781638358856	25.64627963597467	24.056002485190618
25	24.03488448607513	25.836188599034998	25.861591409565225	24.267335505324645
26	23.588651983026026	26.155100991474754	26.340266056183058	23.91598096931616
27	24.562834463962442	26.146684397624377	25.764111949879947	23.526369188533234
28	23.540753912568423	25.845370337780864	26.503701005859483	24.110174743791234
29	23.573043027158054	26.424125936728636	26.253957711971914	23.748873324141393
30	24.12272312007725	26.07812741498858	26.35939467857028	23.439754786363896
31	23.798760771327267	26.278748406585756	25.587057421063825	24.335433401023153
32	23.784988163208467	26.247071407912514	25.648881128619326	24.319059300259692
33	23.491937668236236	25.948358840713663	26.183411352607845	24.376292138442256
34	23.816512132902606	26.107508978975346	26.679990389780112	23.39598849834193
35	25.157045989799087	25.786454180828223	25.7399333711825	23.316566458190188
36	23.719950847091916	26.405609430257808	25.799155586093335	24.07528413655694
37	24.710966515729083	26.2069778153889	25.59440281206052	23.487652856821498
38	23.708167615701388	25.90612284248268	26.185400729336116	24.200308812479822
39	23.942454982700074	26.09098184923279	25.64337208537181	24.32319108269533
40	24.293350431771266	26.161222150638668	26.202693003974165	23.342734413615908
41	23.204090158553324	26.28793014533162	26.555883887731817	23.952095808383234
42	24.726575471597055	26.507373701357828	25.763652862942653	23.00239796410246
43	23.84665884178487	25.486823439754787	26.16917965755175	24.497338060908593
44	23.7139827169071	26.017068852328567	26.245694147100636	24.023254283663697
45	23.930059635393157	26.391377735201715	26.053336720374737	23.62522590903039
46	23.613325783892144	26.022005600869207	26.02185257165593	24.342816043582722
47	23.879866130249088	25.458053991684405	26.54670214898595	24.115377729080556
48	24.26167343309803	26.677082839177256	25.676579373836024	23.384664353888695
49	23.469901495246155	26.008040142561796	26.45044692113345	24.071611441058593
50	23.453986481419985	26.336746389663812	25.85883688794147	24.35043024097473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	12.5
2	21.0
3	37.5
4	54.0
5	76.0
6	98.0
7	102.0
8	106.0
9	145.0
10	184.0
11	236.5
12	289.0
13	433.0
14	577.0
15	747.5
16	918.0
17	1375.0
18	1832.0
19	2208.5
20	2585.0
21	3304.0
22	4023.0
23	4663.0
24	5303.0
25	6964.5
26	8626.0
27	10376.5
28	12127.0
29	13379.5
30	14632.0
31	15073.5
32	15515.0
33	17208.0
34	18901.0
35	20759.0
36	22617.0
37	26272.0
38	29927.0
39	31827.0
40	33727.0
41	36105.0
42	38483.0
43	38489.0
44	38495.0
45	41393.0
46	44291.0
47	46441.0
48	48591.0
49	47988.0
50	47385.0
51	44193.0
52	41001.0
53	39502.5
54	38004.0
55	38022.5
56	38041.0
57	36847.5
58	35654.0
59	31836.0
60	28018.0
61	24959.5
62	21901.0
63	18997.0
64	16093.0
65	14039.5
66	11986.0
67	10869.5
68	9753.0
69	8983.0
70	8213.0
71	6486.0
72	4759.0
73	4330.5
74	3902.0
75	2983.5
76	2065.0
77	1778.0
78	1491.0
79	1345.0
80	1199.0
81	1025.0
82	851.0
83	695.0
84	539.0
85	438.5
86	338.0
87	253.5
88	169.0
89	136.5
90	104.0
91	71.5
92	39.0
93	29.0
94	19.0
95	14.0
96	9.0
97	7.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007498419975790815
2	0.0021424057073688046
3	6.12115916391087E-4
4	0.0
5	0.0
6	1.5302897909777176E-4
7	0.0
8	1.5302897909777176E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5302897909777176E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	653471.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.92682749874123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.81893437949324	58.10515844840878
2	8.75058413256783	11.712976695071786
3	2.002580523452497	4.020790837363325
4	0.7743969972276857	2.0731173699600207
5	0.3843138250502967	1.2860452537261309
6	0.21186972541865404	0.8507861139179956
7	0.15570019485639364	0.7294364057871977
8	0.10937051117921512	0.5855857068112382
9	0.08369681895617315	0.5041406308025858
>10	0.557053810492107	7.516992141066181
>50	0.08778246693179234	4.1557561229621935
>100	0.061883042737529106	7.558791988438215
>500	0.0016043751820549405	0.7312287965685638
>1k	2.291964545792772E-4	0.1691934891158225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1103	0.16879096394484225	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	862	0.13191097998227924	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	793	0.121351980424533	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	792	0.12119895144543523	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	667	0.10207032905821375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.060579581955435E-4
13	0.0	0.0	0.0	0.0	3.060579581955435E-4
14	0.0	0.0	0.0	0.0	3.060579581955435E-4
15	0.0	0.0	0.0	0.0	3.060579581955435E-4
16	0.0	0.0	0.0	0.0	3.060579581955435E-4
17	0.0	0.0	0.0	0.0	3.060579581955435E-4
18	0.0	0.0	0.0	0.0	3.060579581955435E-4
19	0.0	0.0	0.0	0.0	3.060579581955435E-4
20	0.0	0.0	0.0	0.0	4.590869372933152E-4
21	0.0	0.0	0.0	0.0	4.590869372933152E-4
22	0.0	0.0	0.0	0.0	6.12115916391087E-4
23	0.0	0.0	0.0	0.0	6.12115916391087E-4
24	0.0	0.0	0.0	1.5302897909777176E-4	6.12115916391087E-4
25	0.0	0.0	0.0	1.5302897909777176E-4	6.12115916391087E-4
26	1.5302897909777176E-4	0.0	0.0	4.590869372933152E-4	6.12115916391087E-4
27	1.5302897909777176E-4	0.0	0.0	0.0010712028536844023	6.12115916391087E-4
28	1.5302897909777176E-4	0.0	0.0	0.0019893767282710326	6.12115916391087E-4
29	1.5302897909777176E-4	0.0	0.0	0.005049956310226467	6.12115916391087E-4
30	1.5302897909777176E-4	0.0	0.0	0.009793854662257392	6.12115916391087E-4
31	1.5302897909777176E-4	0.0	0.0	0.02096497013639473	6.12115916391087E-4
32	1.5302897909777176E-4	0.0	0.0	0.03810421579534516	6.12115916391087E-4
33	1.5302897909777176E-4	0.0	0.0	0.05692678022437109	6.12115916391087E-4
34	1.5302897909777176E-4	0.0	0.0	0.0855431993156544	7.651448954888587E-4
35	1.5302897909777176E-4	0.0	0.0	0.11660808207250208	7.651448954888587E-4
36	1.5302897909777176E-4	0.0	0.0	0.16175163090634473	7.651448954888587E-4
37	1.5302897909777176E-4	0.0	0.0	0.24270396084906598	7.651448954888587E-4
38	1.5302897909777176E-4	0.0	0.0	0.3427849131790087	7.651448954888587E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATAC	30	0.0057431078	29.333332	1
TAGTACG	45	0.0013975273	24.444445	2
GTATAGA	75	2.0618536E-6	23.466667	1
GTATTAT	85	2.3492248E-7	23.294117	1
CATCCCG	70	3.2124393E-5	22.0	15
TAATACC	55	0.0044814446	20.000002	4
TAACGGC	80	8.9805195E-5	19.250002	36
ATCCCGA	80	8.9805195E-5	19.250002	16
TTAACGG	80	8.9805195E-5	19.250002	35
GCACCGG	70	8.11787E-4	18.857143	6
GTAATAC	70	8.11787E-4	18.857143	3
TATAGGG	70	8.11787E-4	18.857143	4
GTAAACG	140	4.0708983E-9	18.857143	27
CGGTCCA	210	0.0	18.857141	10
TATAGAC	60	0.007408552	18.333332	3
CGCACTG	60	0.007408552	18.333332	5
ATACGAA	205	0.0	18.243904	40
ATTTCGG	85	1.4295593E-4	18.117645	22
TAGCGGC	220	0.0	18.000002	30
CTAGCGG	220	0.0	18.000002	29
>>END_MODULE