FastQCFastQC Report
Thu 2 Feb 2017
SRR4062622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571106
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG33100.5795771713132063No Hit
TCCATGTACTCTGCGTTGATACCAC25640.4489534342136136No Hit
GTATCAACGCAGAGTACTTTTTTTT24010.42041232275619583No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20550.3598281229754196No Hit
TATCAACGCAGAGTACTTTTTTTTT15400.26965221867744343No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14440.2528427297209275No Hit
GAGTACATGGAAGCAGTGGTATCAA14030.24566367714574877No Hit
CATGTACTCTGCGTTGATACCACTG11480.20101347210500328No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10250.1794763143794672No Hit
GCTTCCATGTACTCTGCGTTGATAC8320.14568223762313826No Hit
GGTATCAACGCAGAGTACTTTTTTT8240.14428144687676192No Hit
CATGGAAGCAGTGGTATCAACGCAG7880.13797788851806844No Hit
GTACATGGGGTGGTATCAACGCAAA7640.13377551627893947No Hit
GCGTTGATACCACTGCTTCCATGTA7410.12974824288310752No Hit
CCCATGTACTCTGCGTTGATACCAC6040.10575970135141288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG402.7643674E-416.61941
CCAACGA400.005260932314.255198519
CGACCCG400.00527626414.2489485
AATTGAA400.00527626414.2489487
TACCGTC602.564425E-514.2489487
TGAGTTA1400.013.57161612
ATACCGT707.248802E-613.5704276
GTTTTCG500.001495382913.30251715
ATCCAAA500.001497361213.30018514
ACCGTCG655.44446E-513.1528748
CGAGGAT801.9868876E-613.06611916
TTAACGA801.9927684E-613.06268112
AGGACCT801.994731E-613.0615365
GTTATAT1251.0186341E-1012.9156481
GACGGAC751.4758198E-512.6657327
AGAACCG604.0875163E-412.6657315
CAAGACG751.47715145E-512.6646214
TAAATCC905.39545E-712.664624
TTAAATC1207.385097E-1012.6635093
ATAGGGT1155.313268E-912.3882183
Produced by FastQC (version 0.11.5)