##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062622_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571106
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.939186420734504	32.0	32.0	32.0	32.0	32.0
2	30.573515249358262	32.0	32.0	32.0	32.0	32.0
3	30.576474419809983	32.0	32.0	32.0	32.0	32.0
4	30.627620791937048	32.0	32.0	32.0	32.0	32.0
5	30.41950706173635	32.0	32.0	32.0	32.0	32.0
6	34.025679996357944	36.0	36.0	36.0	32.0	36.0
7	33.8462527096546	36.0	36.0	36.0	32.0	36.0
8	33.81994585943765	36.0	36.0	36.0	32.0	36.0
9	34.016681666800906	36.0	36.0	36.0	32.0	36.0
10	33.638807857035296	36.0	36.0	36.0	27.0	36.0
11	34.072991703816804	36.0	36.0	36.0	32.0	36.0
12	33.79931921569726	36.0	36.0	36.0	32.0	36.0
13	33.952952691794515	36.0	36.0	36.0	32.0	36.0
14	33.830570857248915	36.0	36.0	36.0	32.0	36.0
15	33.79195455834819	36.0	36.0	36.0	32.0	36.0
16	33.76151012246413	36.0	36.0	36.0	32.0	36.0
17	33.683293469163345	36.0	36.0	36.0	27.0	36.0
18	33.713918256856	36.0	36.0	36.0	27.0	36.0
19	33.69405679506081	36.0	36.0	36.0	27.0	36.0
20	33.6609858765273	36.0	36.0	36.0	27.0	36.0
21	33.60923016042556	36.0	36.0	36.0	27.0	36.0
22	33.56734301513204	36.0	36.0	36.0	27.0	36.0
23	33.52753604409689	36.0	36.0	36.0	27.0	36.0
24	33.51905775810445	36.0	36.0	36.0	27.0	36.0
25	33.02005407052281	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	21.0
5	61.0
6	94.0
7	37.0
8	88.0
9	86.0
10	59.0
11	15.0
12	49.0
13	32.0
14	156.0
15	213.0
16	299.0
17	406.0
18	546.0
19	858.0
20	1215.0
21	1820.0
22	2622.0
23	3767.0
24	4784.0
25	6423.0
26	8622.0
27	10787.0
28	14154.0
29	18391.0
30	23176.0
31	31156.0
32	43572.0
33	60915.0
34	127330.0
35	209346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05073301253091	17.672501348350806	11.109710227153334	25.16705541196495
2	15.662762416234305	21.11324544246323	38.11941087785224	25.104581263450225
3	19.281260342191942	25.59207680844991	28.492705963931687	26.633956885426464
4	11.986746384501956	16.10608496464185	36.8103058769137	35.0968627739425
5	13.528336060031739	37.77627527158967	34.03830456578933	14.657084102589254
6	32.28175962244252	36.16594870249655	18.09690151306788	13.455390161993039
7	28.73853432038197	31.489421350037233	21.52630426212274	18.24574006745806
8	27.564342970905027	33.12274146796258	20.629181898796734	18.68373366233566
9	27.52457582709554	14.70886692686928	19.2261922496543	38.54036499638088
10	15.430152105051878	27.505123049603743	32.73002010654541	24.33470473879897
11	36.10456178116843	22.069148069004324	22.68952786976056	19.136762280066684
12	23.800265088292637	24.886566054672706	29.924470875352398	21.38869798168226
13	29.39575032036505	20.547013461467667	25.84307283988058	24.2141633782867
14	23.13686373230301	20.502387647343436	26.32091943706218	30.039829183291378
15	24.623218636610634	27.593998141948152	24.40743921891707	23.375344002524145
16	24.69373410388413	26.60082979696793	25.295135328421857	23.41030077072608
17	22.758405486377846	26.27434343505174	26.95365434206766	24.01359673650275
18	23.339814131412354	25.43044128449535	28.77895102234736	22.45079356174494
19	24.256322594784134	26.598115275038353	26.579005040543503	22.566557089634014
20	24.021001700471576	25.17434216292972	27.810949634486263	22.993706502112442
21	25.26674170146668	25.44854330805292	25.209238396779813	24.075476593700586
22	23.875647922789348	26.18642641210344	26.253761283851556	23.684164381255655
23	23.156661827312195	25.847237710494387	26.37781331313429	24.61828714905913
24	23.109318839309196	26.884369246953625	26.456737091259754	23.549574822477425
25	23.913058722879065	25.863943002201932	26.368318817407893	23.854679457511114
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	222.0
2	444.0
3	666.0
4	666.0
5	666.0
6	1915.5
7	3165.0
8	3165.0
9	3165.0
10	3018.0
11	2871.0
12	2871.0
13	2871.0
14	2657.0
15	2443.0
16	2443.0
17	2443.0
18	3981.0
19	5519.0
20	5519.0
21	5519.0
22	8625.5
23	11732.0
24	11732.0
25	11732.0
26	17106.5
27	22481.0
28	22481.0
29	22481.0
30	27655.0
31	32829.0
32	32829.0
33	32829.0
34	36888.5
35	40948.0
36	40948.0
37	40948.0
38	44411.0
39	47874.0
40	47874.0
41	47874.0
42	54200.5
43	60527.0
44	60527.0
45	60527.0
46	71958.0
47	83389.0
48	83389.0
49	83389.0
50	78150.0
51	72911.0
52	72911.0
53	72911.0
54	64659.0
55	56407.0
56	56407.0
57	56407.0
58	50339.0
59	44271.0
60	44271.0
61	44271.0
62	39261.0
63	34251.0
64	34251.0
65	34251.0
66	28489.0
67	22727.0
68	22727.0
69	22727.0
70	17376.5
71	12026.0
72	12026.0
73	12026.0
74	9345.0
75	6664.0
76	6664.0
77	6664.0
78	5384.0
79	4104.0
80	4104.0
81	4104.0
82	2851.5
83	1599.0
84	1599.0
85	1599.0
86	1212.0
87	825.0
88	825.0
89	825.0
90	555.0
91	285.0
92	285.0
93	285.0
94	187.0
95	89.0
96	89.0
97	89.0
98	185.0
99	281.0
100	281.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006653756045287566
2	0.003326878022643783
3	0.0040272733958319476
4	0.01610909358332779
5	0.036420559405784564
6	0.048852577279874486
7	0.06671265929617269
8	0.08614863090214427
9	0.09192689273094662
10	0.11293875392659157
11	0.11363914929977971
12	0.12782215560684007
13	0.11609053310593831
14	0.11731622500901759
15	0.10786088747097736
16	0.10593480019470991
17	0.11819171922550278
18	0.12309448683781996
19	0.1279972544501371
20	0.11836681806879984
21	0.123269585681117
22	0.14393124919016786
23	0.13692729545828622
24	0.13237472553256313
25	0.12221899262133473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	571106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.045313133276125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.84549688230749	49.506836182309726
2	10.764921486448468	12.712363200449643
3	2.533173460409574	4.487160605723639
4	0.8607669814289728	2.0329702381303427
5	0.42416188520172277	1.252238566546822
6	0.25535872419448097	0.9046641498826214
7	0.16776938159634683	0.6934196969372673
8	0.1306320783443439	0.6170569576873954
9	0.09569027614571642	0.5085056086950139
>10	0.7071217003010118	8.607144055722744
>50	0.11906213201616288	4.881849963901484
>100	0.09108252655701465	9.929081733819348
>500	0.002083587208820575	0.8982659835658092
>1k	0.002678897839912168	2.9684430566281583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3310	0.5795771713132063	No Hit
TCCATGTACTCTGCGTTGATACCAC	2564	0.4489534342136136	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2401	0.42041232275619583	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2055	0.3598281229754196	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1540	0.26965221867744343	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1444	0.2528427297209275	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1403	0.24566367714574877	No Hit
CATGTACTCTGCGTTGATACCACTG	1148	0.20101347210500328	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1025	0.1794763143794672	No Hit
GCTTCCATGTACTCTGCGTTGATAC	832	0.14568223762313826	No Hit
GGTATCAACGCAGAGTACTTTTTTT	824	0.14428144687676192	No Hit
CATGGAAGCAGTGGTATCAACGCAG	788	0.13797788851806844	No Hit
GTACATGGGGTGGTATCAACGCAAA	764	0.13377551627893947	No Hit
GCGTTGATACCACTGCTTCCATGTA	741	0.12974824288310752	No Hit
CCCATGTACTCTGCGTTGATACCAC	604	0.10575970135141288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.750988432970412E-4
2	0.0	0.0	0.0	0.0	1.750988432970412E-4
3	0.0	0.0	0.0	0.0	1.750988432970412E-4
4	0.0	0.0	0.0	0.0	1.750988432970412E-4
5	0.0	0.0	0.0	0.0	1.750988432970412E-4
6	0.0	0.0	0.0	0.0	1.750988432970412E-4
7	0.0	0.0	0.0	0.0	1.750988432970412E-4
8	0.0	0.0	0.0	1.750988432970412E-4	1.750988432970412E-4
9	0.0	0.0	0.0	3.501976865940824E-4	1.750988432970412E-4
10	0.0	0.0	0.0	3.501976865940824E-4	1.750988432970412E-4
11	0.0	0.0	0.0	5.252965298911236E-4	1.750988432970412E-4
12	0.0	0.0	0.0	8.754942164852059E-4	1.750988432970412E-4
13	0.0	0.0	0.0	8.754942164852059E-4	1.750988432970412E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAGG	40	2.7643674E-4	16.6194	1
CCAACGA	40	0.0052609323	14.2551985	19
CGACCCG	40	0.005276264	14.248948	5
AATTGAA	40	0.005276264	14.248948	7
TACCGTC	60	2.564425E-5	14.248948	7
TGAGTTA	140	0.0	13.571616	12
ATACCGT	70	7.248802E-6	13.570427	6
GTTTTCG	50	0.0014953829	13.302517	15
ATCCAAA	50	0.0014973612	13.300185	14
ACCGTCG	65	5.44446E-5	13.152874	8
CGAGGAT	80	1.9868876E-6	13.066119	16
TTAACGA	80	1.9927684E-6	13.062681	12
AGGACCT	80	1.994731E-6	13.061536	5
GTTATAT	125	1.0186341E-10	12.915648	1
GACGGAC	75	1.4758198E-5	12.665732	7
AGAACCG	60	4.0875163E-4	12.665731	5
CAAGACG	75	1.47715145E-5	12.664621	4
TAAATCC	90	5.39545E-7	12.66462	4
TTAAATC	120	7.385097E-10	12.663509	3
ATAGGGT	115	5.313268E-9	12.388218	3
>>END_MODULE