FastQCFastQC Report
Thu 2 Feb 2017
SRR4062622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571106
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12410.2172976645316281No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11950.20924311773996418No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6220.10891148053075962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.10470910829163062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGAC551.5922045E-423.9999523
GCGAGGA1600.023.37495220
TTAGGAC1550.022.7096313
TAAGACC602.8702477E-421.9999564
TATGGCG2950.020.88131516
CGGCTAA856.1042374E-620.7058419
CTAAAAC654.927848E-420.3076523
GATAATT550.00448081719.9999642
TTAGACT550.00448081719.999964
GACCTAC550.00448081719.999967
CTATACA808.978137E-519.249964
CGTCGTA808.978137E-519.2499610
TAGGACG1950.019.179454
TCTTGCG1303.5774065E-818.6153492
TCGCACG951.594734E-518.52627813
CTAAACG951.594734E-518.52627812
TGGCGAG3450.018.49271818
GTTTGCC1203.1547825E-718.33329640
TTTAGAC600.00740752118.3332963
TTAACGG1456.2864274E-918.2068635
Produced by FastQC (version 0.11.5)