##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571106
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14552990162947	32.0	32.0	32.0	32.0	32.0
2	31.372876488777916	32.0	32.0	32.0	32.0	32.0
3	31.489021302525277	32.0	32.0	32.0	32.0	32.0
4	31.601021526651795	32.0	32.0	32.0	32.0	32.0
5	31.530426925999727	32.0	32.0	32.0	32.0	32.0
6	35.1116377695209	36.0	36.0	36.0	36.0	36.0
7	35.11845786946731	36.0	36.0	36.0	36.0	36.0
8	35.07167846249207	36.0	36.0	36.0	36.0	36.0
9	35.1883153039891	36.0	36.0	36.0	36.0	36.0
10	35.08183769738017	36.0	36.0	36.0	36.0	36.0
11	35.19895781168469	36.0	36.0	36.0	36.0	36.0
12	35.11091286030965	36.0	36.0	36.0	36.0	36.0
13	35.148014554215855	36.0	36.0	36.0	36.0	36.0
14	35.10786964241314	36.0	36.0	36.0	36.0	36.0
15	35.087684247757856	36.0	36.0	36.0	36.0	36.0
16	35.08360269372061	36.0	36.0	36.0	36.0	36.0
17	35.0646272320725	36.0	36.0	36.0	36.0	36.0
18	35.06436808578442	36.0	36.0	36.0	36.0	36.0
19	35.0435750981429	36.0	36.0	36.0	36.0	36.0
20	35.016047808988176	36.0	36.0	36.0	36.0	36.0
21	34.99845037523682	36.0	36.0	36.0	36.0	36.0
22	34.98049924182201	36.0	36.0	36.0	36.0	36.0
23	34.936694764194385	36.0	36.0	36.0	32.0	36.0
24	34.92889761270237	36.0	36.0	36.0	32.0	36.0
25	34.90657075919356	36.0	36.0	36.0	32.0	36.0
26	34.83383995265327	36.0	36.0	36.0	32.0	36.0
27	34.83148137123406	36.0	36.0	36.0	32.0	36.0
28	34.78811464071468	36.0	36.0	36.0	32.0	36.0
29	34.76012859259052	36.0	36.0	36.0	32.0	36.0
30	34.73727644255182	36.0	36.0	36.0	32.0	36.0
31	34.72327203706492	36.0	36.0	36.0	32.0	36.0
32	34.66942388978578	36.0	36.0	36.0	32.0	36.0
33	34.632388733440024	36.0	36.0	36.0	32.0	36.0
34	34.58046667343716	36.0	36.0	36.0	32.0	36.0
35	34.51993500330937	36.0	36.0	36.0	32.0	36.0
36	34.486923618382576	36.0	36.0	36.0	32.0	36.0
37	34.43862260245909	36.0	36.0	36.0	32.0	36.0
38	34.38205166816668	36.0	36.0	36.0	32.0	36.0
39	34.31545807608395	36.0	36.0	36.0	32.0	36.0
40	34.272896800243736	36.0	36.0	36.0	32.0	36.0
41	34.24699092637794	36.0	36.0	36.0	32.0	36.0
42	34.15067080366867	36.0	36.0	36.0	32.0	36.0
43	34.161167979324325	36.0	36.0	36.0	32.0	36.0
44	34.12635482730001	36.0	36.0	36.0	32.0	36.0
45	34.00277356567783	36.0	36.0	36.0	32.0	36.0
46	34.024319478345525	36.0	36.0	36.0	32.0	36.0
47	33.94653531918768	36.0	36.0	36.0	32.0	36.0
48	33.887059495084976	36.0	36.0	36.0	32.0	36.0
49	33.89262063434809	36.0	36.0	36.0	32.0	36.0
50	33.33004030775373	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	11.0
21	26.0
22	65.0
23	191.0
24	487.0
25	1085.0
26	2498.0
27	4838.0
28	7724.0
29	11268.0
30	15577.0
31	21565.0
32	30948.0
33	47032.0
34	96889.0
35	330898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63025424945798	18.201480173866138	11.553306924173489	25.61495865250239
2	15.21014348101687	21.568905558882552	37.69747463604431	25.523476324056272
3	18.757978010894746	25.77214888434795	28.728718738016568	26.741154366740734
4	11.918865773774607	16.463580124776094	36.931691831420956	34.68586227002835
5	13.174787167355973	37.92851064425869	34.04236691612415	14.85433527226119
6	32.60048117499151	36.261648532136114	17.659542428497886	13.47832786437449
7	28.4530367392393	31.526721834475563	21.592839157704525	18.427402268580614
8	27.45252194864001	33.16652250195235	20.623491961212103	18.757463588195534
9	27.517133421816613	14.399428477375478	19.075267988779665	39.00817011202824
10	15.331129422559034	27.451296256736928	32.914905464134506	24.302668856569536
11	36.32828231536702	21.83167397996169	22.603509681215044	19.236534023456244
12	23.800485373993617	24.815183170899974	29.974120390960696	21.41021106414571
13	29.580147993542354	20.275745658424178	26.00112763655083	24.142978711482634
14	23.131957990285514	20.293430641597183	26.26640238414585	30.30820898397145
15	24.704520701936243	27.29720927463553	24.50053054949519	23.497739473933034
16	24.716777620967036	26.394574737439285	25.36674452728565	23.521903114308028
17	22.701214835774795	26.053832388383242	27.281100181052203	23.96385259478976
18	23.619082972337885	25.04666384173866	28.76331188956166	22.570941296361795
19	24.214243940704527	26.301247053961962	26.85928706754963	22.62522193778388
20	23.964202792476353	24.82884088067714	28.09548490122674	23.11147142561976
21	25.42067497102114	25.091664244466	25.582641401070905	23.905019383441953
22	23.936362076392122	25.81727385108894	26.522922189576015	23.72344188294292
23	23.136335461367942	25.317541752319183	26.76140681414659	24.78471597216629
24	23.046334655913263	26.56179413278796	26.849131334638404	23.542739876660374
25	24.155760927043314	25.294778902690567	26.63691153656239	23.912548633703725
26	22.722401795813738	25.80361614131177	27.728302626832846	23.745679436041645
27	24.04037078931057	26.212296841567063	26.859637265236223	22.887695103886145
28	23.05946706916054	25.48336035692148	27.357443276729715	24.09972929718826
29	22.907131075492117	26.046653335808063	27.61974134398868	23.42647424471114
30	23.861769969147584	25.790133530377897	27.430634593227875	22.917461907246643
31	23.064544935616155	25.836884921538207	26.8373997121375	24.261170430708134
32	22.850223951420574	26.250643488249114	27.02860764901787	23.870524911312437
33	22.896975342580888	25.65285603723302	27.342034578519574	24.10813404166652
34	22.866333045003906	26.241713447240965	28.15239202529828	22.739561482456846
35	24.334011549519705	25.978014589235627	26.913567708971716	22.77440615227296
36	23.22511057491954	26.360255364153062	26.95839301285576	23.456241048071636
37	23.962451804043383	26.27725851243027	26.70852696347088	23.05176272005547
38	23.244896744212106	26.266577482989145	26.563545121220926	23.924980651577815
39	23.14351451394312	26.11721816965677	26.773313535490782	23.965953780909324
40	24.222123388652893	26.00970747987239	26.71255423686671	23.055614894608006
41	22.803647659103564	26.058910254838857	27.60538323883833	23.532058847219258
42	24.098853802971778	26.839500898257068	26.669830119102233	22.39181517966892
43	23.196044166932232	25.768596372652365	26.741445546010723	24.29391391440468
44	22.835165450897033	26.19023438731164	27.234698987578486	23.739901174212843
45	23.184837840961222	26.328212275829703	26.952614751026953	23.534335132182115
46	23.113747710582626	25.947547390501942	26.718682696382107	24.220022202533332
47	23.449412193183054	25.683148137123407	27.316119949711613	23.55131971998193
48	23.31808806071027	27.32575038609295	25.717992806939517	23.638168746257264
49	22.449072501427057	26.450081070764448	27.320147223107444	23.780699204701055
50	22.31739817126768	27.568612481745948	25.823402310604337	24.29058703638204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	48.5
2	59.0
3	163.5
4	268.0
5	286.5
6	305.0
7	248.5
8	192.0
9	292.0
10	392.0
11	532.5
12	673.0
13	1152.5
14	1632.0
15	2226.5
16	2821.0
17	3279.5
18	3738.0
19	3986.0
20	4234.0
21	4816.5
22	5399.0
23	5465.0
24	5531.0
25	6024.0
26	6517.0
27	8055.0
28	9593.0
29	10538.0
30	11483.0
31	12298.0
32	13113.0
33	14645.0
34	16177.0
35	18630.0
36	21083.0
37	23942.0
38	26801.0
39	27437.5
40	28074.0
41	30074.5
42	32075.0
43	32322.0
44	32569.0
45	35601.5
46	38634.0
47	41078.5
48	43523.0
49	42342.5
50	41162.0
51	38047.0
52	34932.0
53	33517.5
54	32103.0
55	31977.0
56	31851.0
57	31213.0
58	30575.0
59	27772.0
60	24969.0
61	22128.5
62	19288.0
63	16821.5
64	14355.0
65	12422.5
66	10490.0
67	9276.0
68	8062.0
69	7392.5
70	6723.0
71	5340.0
72	3957.0
73	3479.0
74	3001.0
75	2244.0
76	1487.0
77	1326.0
78	1165.0
79	1007.5
80	850.0
81	685.0
82	520.0
83	419.0
84	318.0
85	275.5
86	233.0
87	165.5
88	98.0
89	64.5
90	31.0
91	18.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01610909358332779
2	0.0035019768659408238
3	8.754942164852059E-4
4	5.252965298911236E-4
5	0.0
6	7.003953731881648E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	571106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.97861644529993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.23580481787783	53.892902474041534
2	10.703504298135629	13.695907922220773
3	2.39479197301641	4.596464313236997
4	0.8324603089466011	2.130386352481219
5	0.41118244216778704	1.3153441878247285
6	0.2524960099864797	0.9692607226136164
7	0.16854357196071174	0.7548229181356627
8	0.11126248883836833	0.5694736078511546
9	0.08724591331396737	0.5023685541900784
>10	0.6249691524271206	8.14748643796242
>50	0.10757889135859498	4.782893968164488
>100	0.06850930533593295	7.809541674483146
>500	0.001100551089733691	0.4043393942586759
>1k	5.502755448668455E-4	0.4288074725355396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1241	0.2172976645316281	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1195	0.20924311773996418	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	622	0.10891148053075962	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	598	0.10470910829163062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.750988432970412E-4
11	0.0	0.0	0.0	1.750988432970412E-4	1.750988432970412E-4
12	0.0	0.0	0.0	3.501976865940824E-4	3.501976865940824E-4
13	0.0	0.0	0.0	3.501976865940824E-4	3.501976865940824E-4
14	0.0	0.0	0.0	3.501976865940824E-4	3.501976865940824E-4
15	0.0	0.0	0.0	3.501976865940824E-4	3.501976865940824E-4
16	0.0	0.0	0.0	5.252965298911236E-4	5.252965298911236E-4
17	0.0	0.0	0.0	5.252965298911236E-4	5.252965298911236E-4
18	0.0	0.0	0.0	5.252965298911236E-4	5.252965298911236E-4
19	0.0	0.0	0.0	5.252965298911236E-4	5.252965298911236E-4
20	0.0	0.0	0.0	0.0010505930597822471	5.252965298911236E-4
21	0.0	0.0	0.0	0.0017509884329704119	5.252965298911236E-4
22	0.0	0.0	0.0	0.0036770757092378648	8.754942164852059E-4
23	0.0	0.0	0.0	0.006653756045287565	8.754942164852059E-4
24	0.0	0.0	0.0	0.011556523657604718	8.754942164852059E-4
25	0.0	0.0	0.0	0.015758895896733707	8.754942164852059E-4
26	0.0	0.0	0.0	0.018910675076080446	8.754942164852059E-4
27	0.0	0.0	0.0	0.025739529964665053	8.754942164852059E-4
28	0.0	0.0	0.0	0.03764625130886386	8.754942164852059E-4
29	0.0	0.0	0.0	0.059708705564291045	8.754942164852059E-4
30	0.0	0.0	0.0	0.09472847422369927	8.754942164852059E-4
31	0.0	0.0	0.0	0.16564350575900097	8.754942164852059E-4
32	0.0	0.0	0.0	0.2530178285642245	8.754942164852059E-4
33	0.0	0.0	0.0	0.32813523233865516	8.754942164852059E-4
34	0.0	0.0	0.0	0.4193617296964136	0.0010505930597822471
35	0.0	0.0	0.0	0.5177672796293508	0.0010505930597822471
36	0.0	0.0	0.0	0.6618736276628157	0.0010505930597822471
37	0.0	0.0	0.0	0.8614863090214426	0.0010505930597822471
38	0.0	0.0	0.0	1.1113523584063203	0.0010505930597822471
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGAC	55	1.5922045E-4	23.999952	3
GCGAGGA	160	0.0	23.374952	20
TTAGGAC	155	0.0	22.709631	3
TAAGACC	60	2.8702477E-4	21.999956	4
TATGGCG	295	0.0	20.881315	16
CGGCTAA	85	6.1042374E-6	20.705841	9
CTAAAAC	65	4.927848E-4	20.307652	3
GATAATT	55	0.004480817	19.99996	42
TTAGACT	55	0.004480817	19.99996	4
GACCTAC	55	0.004480817	19.99996	7
CTATACA	80	8.978137E-5	19.24996	4
CGTCGTA	80	8.978137E-5	19.24996	10
TAGGACG	195	0.0	19.17945	4
TCTTGCG	130	3.5774065E-8	18.615349	2
TCGCACG	95	1.594734E-5	18.526278	13
CTAAACG	95	1.594734E-5	18.526278	12
TGGCGAG	345	0.0	18.492718	18
GTTTGCC	120	3.1547825E-7	18.333296	40
TTTAGAC	60	0.007407521	18.333296	3
TTAACGG	145	6.2864274E-9	18.20686	35
>>END_MODULE