FastQCFastQC Report
Thu 2 Feb 2017
SRR4062621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8241
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT170.2062856449460017No Hit
GCGCAAGACGGACCAGAGCGAAAGC160.19415119524329572No Hit
GTACATGGGTACCTGGTTGATCCTG150.18201674554058975No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT150.18201674554058975No Hit
GGCTCCACTCCTGGTGGTGCCCTTC140.16988229583788375No Hit
GGCGGTGACTCGCCTCGCGGCGGAC140.16988229583788375No Hit
GTATCTGATCGTCTTCGAACCTCCG130.15774784613517778No Hit
CTCCACTCCTGGTGGTGCCCTTCCG130.15774784613517778No Hit
CTATTGGAGCTGGAATTACCGCGGC110.13347894672976582No Hit
GAACTACGACGGTATCTGATCGTCT110.13347894672976582No Hit
TTCGTATTGCGCCGCTAGAGGTGAA110.13347894672976582No Hit
CTCCGAGGTCACCCCAACCGAAATT110.13347894672976582No Hit
TATCAACGCAGAGTACTTTTTTTTT110.13347894672976582No Hit
GTCCTGTATTGTTATTTTTCGTCAC100.12134449702705982No Hit
GAGTACTTTTTTTTTTTTTTTTTTT100.12134449702705982No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG100.12134449702705982No Hit
GGACTCTTTCGAGGCCCTGTAATTG90.10921004732435385No Hit
GAATAACGCCGCCGCATCGCCAGTC90.10921004732435385No Hit
GCATATGCTTGTCTCAAAGATTAAG90.10921004732435385No Hit
GCTAAGAGCATCGAGGGGGCGCCGA90.10921004732435385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG90.10921004732435385No Hit
GGGCGGGGACGGGCGGTGACTCGCC90.10921004732435385No Hit
TCGTAGTTCCGACCATAAACGATGC90.10921004732435385No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)