##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062621_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8241
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.003883023904866	32.0	32.0	32.0	21.0	32.0
2	28.963717995388908	32.0	32.0	32.0	14.0	32.0
3	29.19439388423735	32.0	32.0	32.0	14.0	32.0
4	28.958257493022693	32.0	32.0	32.0	14.0	32.0
5	28.633782307972332	32.0	32.0	32.0	14.0	32.0
6	31.91457347409295	36.0	32.0	36.0	14.0	36.0
7	32.17643489867734	36.0	32.0	36.0	14.0	36.0
8	31.898677344982406	36.0	32.0	36.0	14.0	36.0
9	32.15714112365004	36.0	32.0	36.0	14.0	36.0
10	31.341948792622254	36.0	32.0	36.0	14.0	36.0
11	32.526756461594466	36.0	32.0	36.0	21.0	36.0
12	31.875621890547265	36.0	32.0	36.0	14.0	36.0
13	32.197427496663025	36.0	32.0	36.0	14.0	36.0
14	31.88278121587186	36.0	32.0	36.0	14.0	36.0
15	31.773935202038587	36.0	32.0	36.0	14.0	36.0
16	31.667637422642883	36.0	32.0	36.0	14.0	36.0
17	31.54107511224366	36.0	32.0	36.0	14.0	36.0
18	31.61072685353719	36.0	32.0	36.0	14.0	36.0
19	31.623346681228007	36.0	32.0	36.0	14.0	36.0
20	31.604780973182866	36.0	32.0	36.0	14.0	36.0
21	31.619827690814223	36.0	32.0	36.0	14.0	36.0
22	31.42931683048174	36.0	32.0	36.0	14.0	36.0
23	31.400800873680378	36.0	32.0	36.0	14.0	36.0
24	31.34085669214901	36.0	32.0	36.0	14.0	36.0
25	30.693969178497756	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	0.0
7	0.0
8	2.0
9	0.0
10	2.0
11	0.0
12	0.0
13	0.0
14	7.0
15	9.0
16	13.0
17	23.0
18	32.0
19	60.0
20	84.0
21	94.0
22	143.0
23	156.0
24	182.0
25	211.0
26	275.0
27	324.0
28	432.0
29	434.0
30	494.0
31	685.0
32	831.0
33	972.0
34	1424.0
35	1350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73037252760587	18.4564979978158	11.38211382113821	28.431015653440117
2	17.51001092100473	19.961169760951343	37.204222788496544	25.32459652954738
3	18.589976944545565	23.176798932168428	28.33394005581847	29.89928406746754
4	12.246631872800098	14.51632479669863	36.084476271392155	37.15256705910912
5	14.734797912368006	36.24226241048671	33.59631023182425	15.426629445321034
6	34.64012622891128	34.66440101954121	16.579682000242748	14.115790751304768
7	30.860750273157706	30.22945247055967	20.32293310671361	18.586864149569017
8	27.45264691597863	32.673627974745024	19.65760077707625	20.216124332200096
9	26.49987855234394	13.796453728443042	18.848676220548942	40.85499149866408
10	14.72124377505162	25.87149277298676	32.45475525324912	26.9525081987125
11	37.28134110787172	19.910106899902818	22.95918367346939	19.849368318756074
12	23.697315680796795	23.41795214381149	29.10239280942548	23.782339365966234
13	28.500303582270796	19.247115968427444	24.845173041894352	27.40740740740741
14	23.536555744474132	18.872965751760994	25.977653631284912	31.612824872479962
15	24.981782851590964	26.876366286130676	22.297789652659702	25.84406120961865
16	25.273257226135538	26.305562302647562	24.058780665533156	24.36239980568375
17	22.792956891317548	26.569520340012144	25.78020643594414	24.857316332726167
18	24.462528847321753	24.6811611806146	26.746022106158147	24.1102878659055
19	24.553734061930783	25.54948391013965	25.889496053430477	24.00728597449909
20	25.458520587878052	24.09814162516701	25.44637434713956	24.996963439815378
21	25.905224787363306	23.608748481166465	25.32199270959903	25.164034021871203
22	24.31972789115646	25.35228377065112	25.801749271137027	24.526239067055393
23	23.748785228377063	24.31972789115646	25.89893100097182	26.032555879494655
24	24.96963808598494	24.568860820986156	26.329851833859607	24.1316492591693
25	24.207457791813432	24.122434106643993	25.579982995262966	26.090125106279604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.5
3	5.0
4	5.0
5	5.0
6	12.5
7	20.0
8	20.0
9	20.0
10	23.5
11	27.0
12	27.0
13	27.0
14	26.0
15	25.0
16	25.0
17	25.0
18	36.0
19	47.0
20	47.0
21	47.0
22	99.0
23	151.0
24	151.0
25	151.0
26	202.5
27	254.0
28	254.0
29	254.0
30	323.5
31	393.0
32	393.0
33	393.0
34	494.5
35	596.0
36	596.0
37	596.0
38	615.5
39	635.0
40	635.0
41	635.0
42	744.5
43	854.0
44	854.0
45	854.0
46	922.5
47	991.0
48	991.0
49	991.0
50	1043.0
51	1095.0
52	1095.0
53	1095.0
54	992.0
55	889.0
56	889.0
57	889.0
58	829.0
59	769.0
60	769.0
61	769.0
62	668.5
63	568.0
64	568.0
65	568.0
66	479.5
67	391.0
68	391.0
69	391.0
70	307.0
71	223.0
72	223.0
73	223.0
74	175.0
75	127.0
76	127.0
77	127.0
78	106.5
79	86.0
80	86.0
81	86.0
82	67.5
83	49.0
84	49.0
85	49.0
86	38.5
87	28.0
88	28.0
89	28.0
90	17.5
91	7.0
92	7.0
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	5.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.024268899405411964
5	0.024268899405411964
6	0.024268899405411964
7	0.04853779881082393
8	0.06067224851352991
9	0.08494114791894188
10	0.09707559762164786
11	0.10921004732435385
12	0.09707559762164786
13	0.0728066982162359
14	0.08494114791894188
15	0.08494114791894188
16	0.08494114791894188
17	0.0728066982162359
18	0.09707559762164786
19	0.0728066982162359
20	0.09707559762164786
21	0.13347894672976582
22	0.10921004732435385
23	0.10921004732435385
24	0.08494114791894188
25	0.09707559762164786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8241.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.11321441572625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.55063379300739	81.49496420337337
2	3.635603844546594	6.334182744812522
3	1.2397269814737428	3.2398980706224974
4	0.5014625992478061	1.7473607571896617
5	0.2785903329154478	1.2134449702705983
6	0.23680178297813068	1.2377138696760102
7	0.1532246831034963	0.9343526271083606
8	0.08357709987463435	0.5824535857298871
9	0.09750661652040675	0.7644703312704768
>10	0.22287226633235824	2.451158839946608
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17	0.2062856449460017	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	16	0.19415119524329572	No Hit
GTACATGGGTACCTGGTTGATCCTG	15	0.18201674554058975	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	15	0.18201674554058975	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	14	0.16988229583788375	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	14	0.16988229583788375	No Hit
GTATCTGATCGTCTTCGAACCTCCG	13	0.15774784613517778	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	13	0.15774784613517778	No Hit
CTATTGGAGCTGGAATTACCGCGGC	11	0.13347894672976582	No Hit
GAACTACGACGGTATCTGATCGTCT	11	0.13347894672976582	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	11	0.13347894672976582	No Hit
CTCCGAGGTCACCCCAACCGAAATT	11	0.13347894672976582	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11	0.13347894672976582	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	10	0.12134449702705982	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	10	0.12134449702705982	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	10	0.12134449702705982	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	9	0.10921004732435385	No Hit
GAATAACGCCGCCGCATCGCCAGTC	9	0.10921004732435385	No Hit
GCATATGCTTGTCTCAAAGATTAAG	9	0.10921004732435385	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	9	0.10921004732435385	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	9	0.10921004732435385	No Hit
GGGCGGGGACGGGCGGTGACTCGCC	9	0.10921004732435385	No Hit
TCGTAGTTCCGACCATAAACGATGC	9	0.10921004732435385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE