##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8241
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.667637422642883	32.0	32.0	32.0	21.0	32.0
2	29.50928285402257	32.0	32.0	32.0	14.0	32.0
3	30.552481494964205	32.0	32.0	32.0	32.0	32.0
4	31.043077296444608	32.0	32.0	32.0	32.0	32.0
5	30.81676980948914	32.0	32.0	32.0	32.0	32.0
6	34.365125591554424	36.0	36.0	36.0	32.0	36.0
7	34.3636694575901	36.0	36.0	36.0	32.0	36.0
8	34.32350442907414	36.0	36.0	36.0	32.0	36.0
9	34.675039436961534	36.0	36.0	36.0	32.0	36.0
10	34.18323019051086	36.0	36.0	36.0	32.0	36.0
11	34.797718723455894	36.0	36.0	36.0	32.0	36.0
12	34.502244873195	36.0	36.0	36.0	32.0	36.0
13	34.701856570804516	36.0	36.0	36.0	32.0	36.0
14	34.56012619827691	36.0	36.0	36.0	32.0	36.0
15	34.59264652348016	36.0	36.0	36.0	32.0	36.0
16	34.52068923674312	36.0	36.0	36.0	32.0	36.0
17	34.43684018929741	36.0	36.0	36.0	32.0	36.0
18	34.536706710350686	36.0	36.0	36.0	32.0	36.0
19	34.50891882053149	36.0	36.0	36.0	32.0	36.0
20	34.49981798325446	36.0	36.0	36.0	32.0	36.0
21	34.58512316466448	36.0	36.0	36.0	32.0	36.0
22	34.53488654289528	36.0	36.0	36.0	32.0	36.0
23	34.3694939934474	36.0	36.0	36.0	32.0	36.0
24	34.32763014197306	36.0	36.0	36.0	32.0	36.0
25	34.41038708894552	36.0	36.0	36.0	32.0	36.0
26	34.36403349108118	36.0	36.0	36.0	32.0	36.0
27	34.2932896493144	36.0	36.0	36.0	32.0	36.0
28	34.35723819924766	36.0	36.0	36.0	32.0	36.0
29	34.28200461109089	36.0	36.0	36.0	32.0	36.0
30	34.275330663754396	36.0	36.0	36.0	32.0	36.0
31	34.161752214537074	36.0	36.0	36.0	32.0	36.0
32	34.02002184200946	36.0	36.0	36.0	32.0	36.0
33	34.11721878412814	36.0	36.0	36.0	32.0	36.0
34	34.115398616672735	36.0	36.0	36.0	32.0	36.0
35	34.035553937628926	36.0	36.0	36.0	32.0	36.0
36	33.93847834000728	36.0	36.0	36.0	32.0	36.0
37	33.96226186142459	36.0	36.0	36.0	32.0	36.0
38	34.11940298507463	36.0	36.0	36.0	32.0	36.0
39	33.9426040529062	36.0	36.0	36.0	32.0	36.0
40	33.92767867977187	36.0	36.0	36.0	32.0	36.0
41	33.87768474699672	36.0	36.0	36.0	32.0	36.0
42	33.75415604902318	36.0	36.0	36.0	32.0	36.0
43	33.737167819439385	36.0	36.0	36.0	32.0	36.0
44	33.6318407960199	36.0	36.0	36.0	27.0	36.0
45	33.75100109210047	36.0	36.0	36.0	32.0	36.0
46	33.676738259919915	36.0	36.0	36.0	27.0	36.0
47	33.8278121587186	36.0	36.0	36.0	32.0	36.0
48	33.79990292440238	36.0	36.0	36.0	32.0	36.0
49	33.62225458075476	36.0	36.0	36.0	27.0	36.0
50	33.017473607571894	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	4.0
23	4.0
24	10.0
25	24.0
26	39.0
27	77.0
28	146.0
29	209.0
30	313.0
31	530.0
32	720.0
33	1099.0
34	2016.0
35	3049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.533430408930954	18.165271204950855	12.29219754884116	25.009100837277032
2	20.009707559762166	19.585001820167456	34.922946244387816	25.482344375682565
3	21.86627836427618	22.388059701492537	28.746511345710474	26.99915058852081
4	15.653440116490716	15.835456862031307	36.561096954253124	31.95000606722485
5	19.0632204829511	33.2726610848198	31.56170367673826	16.102414755490837
6	36.13639121465842	35.72381992476642	15.362213323625774	12.777575536949398
7	30.95498119160296	30.433199854386604	19.196699429680862	19.415119524329572
8	28.27326780730494	33.4668122800631	17.88617886178862	20.373741050843343
9	25.797840067952922	12.680499939327753	17.81337216357238	43.70828782914695
10	15.786919063220484	26.222545807547625	30.603082150224488	27.387452979007403
11	38.3205921611455	19.439388423734982	21.52651377260041	20.713505642519113
12	24.00194151195243	23.128261133357604	27.884965416818346	24.98483193787162
13	28.13978886057517	18.238077903167092	26.234680257250332	27.387452979007403
14	23.067588884844074	19.16029608057275	24.681470695303968	33.090644339279216
15	25.555151073898795	25.943453464385392	21.514379322897707	26.987016138818102
16	25.579419973304212	26.028394612304336	23.140395583060307	25.251789831331152
17	24.06261376046596	26.744327144763986	24.57226064797961	24.620798446790438
18	25.664361121223152	24.414512801844438	25.664361121223152	24.256764955709258
19	25.288193180439265	25.1547142337095	25.555151073898795	24.00194151195243
20	25.17898313311491	25.094041985195968	24.378109452736318	25.3488654289528
21	26.865671641791046	24.00194151195243	24.06261376046596	25.06977308579056
22	24.681470695303968	24.85135299114185	25.1547142337095	25.31246207984468
23	23.637908020871254	24.839218541439145	25.555151073898795	25.967722363790802
24	24.48731950006067	25.02123528697974	24.91202523965538	25.579419973304212
25	24.754277393520205	25.49447882538527	24.147554908384905	25.603688872709622
26	24.620798446790438	25.21538648222303	25.1547142337095	25.009100837277032
27	25.409537677466325	24.79068074262832	24.875621890547265	24.92415968935809
28	24.244630506006555	25.78570561825021	25.47020992597986	24.499453949763378
29	25.16684868341221	24.60866399708773	24.57226064797961	25.652226671520445
30	24.36597500303361	26.089066860817862	25.1547142337095	24.390243902439025
31	25.05763863608785	24.438781701249848	25.7614367188448	24.7421429438175
32	24.887756340249968	25.51874772479068	24.12328600897949	25.47020992597986
33	23.34668122800631	24.305302754520085	26.12547020992598	26.222545807547625
34	25.56728552360151	24.086882659871375	26.42883145249363	23.91700036403349
35	26.21041135784492	24.79068074262832	24.79068074262832	24.208227156898435
36	24.135420458682198	25.579419973304212	25.094041985195968	25.19111758281762
37	25.943453464385392	24.256764955709258	24.98483193787162	24.814949642033735
38	24.5358572988715	24.73000849411479	26.076932411115155	24.657201795898555
39	25.603688872709622	25.276058730736562	23.747118068195608	25.37313432835821
40	26.28321805606116	25.16684868341221	24.48731950006067	24.06261376046596
41	24.511588399466085	24.996966387574325	25.506613275087975	24.98483193787162
42	25.870646766169152	25.627957772115035	25.02123528697974	23.480160174736074
43	24.28103385511467	24.256764955709258	26.052663511709746	25.409537677466325
44	24.7421429438175	25.530882174493385	24.64506734619585	25.081907535493265
45	24.050479310763258	25.749302269142092	24.91202523965538	25.288193180439265
46	24.450916150952555	24.111151559276784	25.21538648222303	26.222545807547625
47	23.965538162844314	25.33673097925009	25.71289892003398	24.98483193787162
48	25.397403227763622	26.12547020992598	24.06261376046596	24.414512801844438
49	24.232496056303845	26.028394612304336	24.754277393520205	24.98483193787162
50	25.142579784006795	25.482344375682565	24.98483193787162	24.390243902439025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	2.0
12	4.0
13	5.5
14	7.0
15	11.5
16	16.0
17	21.5
18	27.0
19	24.0
20	21.0
21	29.0
22	37.0
23	44.5
24	52.0
25	59.5
26	67.0
27	77.5
28	88.0
29	111.5
30	135.0
31	147.0
32	159.0
33	175.5
34	192.0
35	215.5
36	239.0
37	281.5
38	324.0
39	344.5
40	365.0
41	402.5
42	440.0
43	418.5
44	397.0
45	455.5
46	514.0
47	550.0
48	586.0
49	567.5
50	549.0
51	527.0
52	505.0
53	515.0
54	525.0
55	532.0
56	539.0
57	536.0
58	533.0
59	478.0
60	423.0
61	397.5
62	372.0
63	332.5
64	293.0
65	244.5
66	196.0
67	185.5
68	175.0
69	159.0
70	143.0
71	123.0
72	103.0
73	80.0
74	57.0
75	46.0
76	35.0
77	31.0
78	27.0
79	30.5
80	34.0
81	26.5
82	19.0
83	12.5
84	6.0
85	7.0
86	8.0
87	8.0
88	8.0
89	4.5
90	1.0
91	2.0
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	5.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8241.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.37022206042955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.25661914460285	84.23734983618493
2	3.367277664630007	6.018687052542168
3	1.1541072640868975	3.0942846741900256
4	0.40733197556008144	1.456133964324718
5	0.25797691785471827	1.1527727217570682
6	0.1493550577053632	0.8008736803785949
7	0.20366598778004072	1.274117218784128
8	0.05431093007467753	0.38830239048659143
9	0.05431093007467753	0.4368401892974154
>10	0.09504412763068568	1.1406382720543622
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	18	0.2184200946487077	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	14	0.16988229583788375	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	14	0.16988229583788375	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	14	0.16988229583788375	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	12	0.1456133964324718	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	12	0.1456133964324718	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	10	0.12134449702705982	No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA	9	0.10921004732435385	No Hit
AATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA	9	0.10921004732435385	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	9	0.10921004732435385	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT	9	0.10921004732435385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.012134449702705982	0.0
33	0.0	0.0	0.0	0.024268899405411964	0.0
34	0.0	0.0	0.0	0.03640334910811795	0.0
35	0.0	0.0	0.0	0.04853779881082393	0.0
36	0.0	0.0	0.0	0.0728066982162359	0.0
37	0.0	0.0	0.0	0.18201674554058975	0.0
38	0.0	0.0	0.0	0.23055454435141368	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE