FastQCFastQC Report
Thu 2 Feb 2017
SRR4062620_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062620_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453514
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG63020.4335699552945482No Hit
TCCATGTACTCTGCGTTGATACCAC51030.3510802097537416No Hit
GAGTACATGGAAGCAGTGGTATCAA28740.19772771366495268No Hit
GTATCAACGCAGAGTACTTTTTTTT26390.1815599987341023No Hit
CATGTACTCTGCGTTGATACCACTG23630.16257153353872064No Hit
TATCAACGCAGAGTACTTTTTTTTT19310.13285045758073194No Hit
GCTTCCATGTACTCTGCGTTGATAC16180.11131643726857807No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15770.10849568700404674No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15450.1062941258219735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA3350.013.89625310
TCGCCAG1750.013.57588717
ACGGTAT2400.013.4590429
GGTTTAA1153.8198777E-1013.2062521
CGGAATT604.0839764E-412.66951715
CGAACGT604.0982512E-412.6642784
TAGGACA2050.012.5098354
ATCGCCA1950.012.18348816
TGTAGGA2850.011.9915382
AGAATTT4300.011.93415117
CGAACGA802.8564371E-511.87890116
GCATCGC2100.011.76495714
GGTATCT2750.011.74364411
GTCCTAA2200.011.6492651
CGCCGGT3600.011.6117227
TCTTATA907.5183016E-611.6029232
GCGCCGA2050.011.5891719
GAATTTC4550.011.48768318
AGTTAAA2400.011.48293717
CCGGTCC4150.011.4463859
Produced by FastQC (version 0.11.5)