FastQCFastQC Report
Thu 2 Feb 2017
SRR4062620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453514
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24430.16807543649390375No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21850.15032534946343826No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14950.10285418647498408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACG956.868795E-720.84194237
TCGTATA654.933083E-420.30753344
CTTACGC550.004484242819.9998463
AGTCGTA708.124829E-418.85699742
CGCAATA4350.018.71249836
ATACGAA4350.018.71249840
TAATACC2250.018.5776334
CGATTAA600.007413138618.3331941
ACGATTA600.007413138618.3331940
GTGTACG851.4277802E-418.1231161
GTACCGT851.4311704E-418.1175066
ATAACGC4250.018.1175043
GGCGTTA3300.017.9998642
TAACGCC4400.017.999864
GTATAGA1355.5088094E-817.9313371
CGTCGTA3350.017.73120510
CTAGCGG4650.017.5052429
GCGTAAC2150.017.39521211
CAATACG4850.017.23697738
ACCGTCG3200.017.1873668
Produced by FastQC (version 0.11.5)