##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062620_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1453514 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25313756867839 32.0 32.0 32.0 32.0 32.0 2 31.360562058569784 32.0 32.0 32.0 32.0 32.0 3 31.473442980253374 32.0 32.0 32.0 32.0 32.0 4 31.593518191087256 32.0 32.0 32.0 32.0 32.0 5 31.50075265872912 32.0 32.0 32.0 32.0 32.0 6 35.1045892918816 36.0 36.0 36.0 36.0 36.0 7 35.126834691650714 36.0 36.0 36.0 36.0 36.0 8 35.05749308228197 36.0 36.0 36.0 36.0 36.0 9 35.20287179896444 36.0 36.0 36.0 36.0 36.0 10 35.072393523557395 36.0 36.0 36.0 36.0 36.0 11 35.20417072006186 36.0 36.0 36.0 36.0 36.0 12 35.10796593634461 36.0 36.0 36.0 36.0 36.0 13 35.16441671700444 36.0 36.0 36.0 36.0 36.0 14 35.11996788472626 36.0 36.0 36.0 36.0 36.0 15 35.074381120512086 36.0 36.0 36.0 36.0 36.0 16 35.08993515026343 36.0 36.0 36.0 36.0 36.0 17 35.0611366660383 36.0 36.0 36.0 36.0 36.0 18 35.06960579671059 36.0 36.0 36.0 36.0 36.0 19 35.0545608779826 36.0 36.0 36.0 36.0 36.0 20 35.05259116871251 36.0 36.0 36.0 36.0 36.0 21 35.04258438515212 36.0 36.0 36.0 36.0 36.0 22 35.02358284818722 36.0 36.0 36.0 36.0 36.0 23 34.96457963253192 36.0 36.0 36.0 32.0 36.0 24 34.943031852462376 36.0 36.0 36.0 32.0 36.0 25 34.917263954801946 36.0 36.0 36.0 32.0 36.0 26 34.859941493511585 36.0 36.0 36.0 32.0 36.0 27 34.83887943287784 36.0 36.0 36.0 32.0 36.0 28 34.818321667352365 36.0 36.0 36.0 32.0 36.0 29 34.78796970651813 36.0 36.0 36.0 32.0 36.0 30 34.77317590336247 36.0 36.0 36.0 32.0 36.0 31 34.76495307234743 36.0 36.0 36.0 32.0 36.0 32 34.72019877345522 36.0 36.0 36.0 32.0 36.0 33 34.70443903533093 36.0 36.0 36.0 32.0 36.0 34 34.68215442025326 36.0 36.0 36.0 32.0 36.0 35 34.65218842061377 36.0 36.0 36.0 32.0 36.0 36 34.61013653807256 36.0 36.0 36.0 32.0 36.0 37 34.58705936096935 36.0 36.0 36.0 32.0 36.0 38 34.54685885378469 36.0 36.0 36.0 32.0 36.0 39 34.49963261447774 36.0 36.0 36.0 32.0 36.0 40 34.460954624448064 36.0 36.0 36.0 32.0 36.0 41 34.44521621394772 36.0 36.0 36.0 32.0 36.0 42 34.38076895028187 36.0 36.0 36.0 32.0 36.0 43 34.38972586435356 36.0 36.0 36.0 32.0 36.0 44 34.36972192906295 36.0 36.0 36.0 32.0 36.0 45 34.27623194547834 36.0 36.0 36.0 32.0 36.0 46 34.32344648899151 36.0 36.0 36.0 32.0 36.0 47 34.27394025788537 36.0 36.0 36.0 32.0 36.0 48 34.24652256531413 36.0 36.0 36.0 32.0 36.0 49 34.255928735464536 36.0 36.0 36.0 32.0 36.0 50 33.700066184433034 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 11.0 21 36.0 22 125.0 23 365.0 24 1073.0 25 2433.0 26 5163.0 27 9943.0 28 16476.0 29 24334.0 30 34991.0 31 49781.0 32 74445.0 33 117816.0 34 259377.0 35 857144.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.95115956468847 17.301335439797867 11.309609001282595 26.437895994231077 2 16.259241880286606 20.06071122400818 36.073365358549864 27.606681537155353 3 19.055593204013746 23.31921802814576 28.30482179283869 29.320366975001804 4 12.473116800022016 15.219709530722184 35.17898053676961 37.12819313248619 5 14.488680535584797 36.421596214415544 33.44315913021821 15.64656411978144 6 35.91293333984631 34.36137562461 16.023108247844693 13.702582787698994 7 30.720833402132214 30.103638635250345 20.365363340653534 18.810164621963903 8 28.739317268357922 32.771958164833634 19.12110925660159 19.36761531020685 9 27.170016938261345 13.972070444453925 18.014480768675085 40.84343184860965 10 15.63087799635917 26.869847830843046 31.672966342257453 25.82630783054033 11 38.30633898263106 20.670182743337868 21.18796241384672 19.83551586018435 12 24.22763041842046 23.42454217847231 28.711935351155887 23.63589205195134 13 30.223031907501408 18.90446187652819 24.74905642463712 26.123449791333282 14 23.516594955397746 19.060015933799054 24.76474254805939 32.658646562743805 15 25.749872378250227 26.620589825760195 22.284752675240828 25.344785120748753 16 26.51697885262887 25.312243294526233 23.458391181646686 24.712386671198214 17 24.422262186673123 25.526964308565308 25.284104590667855 24.766668914093707 18 25.57663703273584 24.11817154839926 26.182272754166796 24.122918664698105 19 25.645504618462567 25.349738633408414 24.7296551667201 24.27510158140892 20 25.469173327535888 24.040566516731175 25.529165869747384 24.96109428598555 21 26.484437026406347 24.363920815348184 24.00038802515834 25.151254133087125 22 25.329649387622 24.512182201203427 24.67454733838133 25.483621072793245 23 24.34899147858225 24.047927986933733 25.08369372431225 26.519386810171763 24 24.696631748989002 25.292291646313693 24.79707797792109 25.213998626776213 25 25.41268952345832 24.117689956890683 24.636295212842807 25.83332530680819 26 24.603890984194166 24.87557739381939 25.2959379820215 25.224593639964944 27 25.802503450259167 24.623773833619765 24.584833720211847 24.988888995909225 28 24.86945430178175 24.102967016485564 25.268143272097827 25.759435409634857 29 24.58393933598163 24.93797789357378 25.588332826515604 24.889749943928987 30 25.240623757321913 24.5406648989965 25.472338071735116 24.74637327194647 31 24.910595976371745 24.65438929380797 24.63505683467789 25.799957895142395 32 24.815034461312376 24.708877933064286 24.724908050421256 25.751179555202082 33 24.56130453507844 24.171834602212293 25.255277898940086 26.011582963769182 34 24.93832188750848 24.69842051744944 25.622319427263857 24.740938167778225 35 25.937073877513388 24.351468234912083 25.045716793921493 24.665741093653036 36 24.836018091329013 25.027966706891025 24.96804296346647 25.167972238313496 37 25.88478679943915 24.501311992866942 24.844411543335667 24.76948966435824 38 24.985311458988356 24.503100761327374 24.96824935982729 25.54333841985698 39 25.20044526574907 24.369837511025004 24.945889754071857 25.483827469154065 40 25.553727036684887 24.524634781639527 25.013794156781426 24.907844024894153 41 24.408571228072105 24.786207769584607 25.611311621353494 25.193909380989794 42 26.0316722095556 24.76295377959896 24.973478067634712 24.23189594321073 43 25.050051117498697 23.932483622448768 24.932817984553296 26.08464727549924 44 24.766118523798188 24.487277040331225 25.545815176186814 25.200789259683777 45 25.02707232266081 24.676473704415645 25.066356430003427 25.23009754292012 46 24.5153806779717 24.42731810951681 25.599169459901262 25.45813175261023 47 25.014138150716125 24.086868100341654 25.845227496948773 25.05376625199344 48 25.25981861887811 25.447088916928216 24.473998874451848 24.819093589741826 49 24.28948052787933 24.770315249801516 25.662291522475876 25.277912699843274 50 24.180984840875286 25.647086990562183 24.63471284074319 25.53721532781934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 13.5 2 16.0 3 96.5 4 177.0 5 203.5 6 230.0 7 228.5 8 227.0 9 306.0 10 385.0 11 542.5 12 700.0 13 1217.0 14 1734.0 15 2318.0 16 2902.0 17 3389.0 18 3876.0 19 4195.0 20 4514.0 21 5343.0 22 6172.0 23 6597.5 24 7023.0 25 8254.0 26 9485.0 27 11475.5 28 13466.0 29 15342.5 30 17219.0 31 18686.0 32 20153.0 33 23124.5 34 26096.0 35 30275.5 36 34455.0 37 41110.5 38 47766.0 39 52357.0 40 56948.0 41 62577.0 42 68206.0 43 71696.5 44 75187.0 45 85981.0 46 96775.0 47 105586.0 48 114397.0 49 115248.5 50 116100.0 51 111712.5 52 107325.0 53 105588.5 54 103852.0 55 105000.0 56 106148.0 57 103793.5 58 101439.0 59 92692.5 60 83946.0 61 74620.0 62 65294.0 63 57069.0 64 48844.0 65 41746.0 66 34648.0 67 29926.5 68 25205.0 69 22672.5 70 20140.0 71 15631.0 72 11122.0 73 9899.5 74 8677.0 75 6442.0 76 4207.0 77 3668.0 78 3129.0 79 2667.0 80 2205.0 81 1742.0 82 1279.0 83 1105.0 84 931.0 85 730.0 86 529.0 87 395.0 88 261.0 89 162.5 90 64.0 91 46.0 92 28.0 93 19.0 94 10.0 95 5.5 96 1.0 97 3.0 98 5.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014447745257355623 2 0.00522870780742394 3 6.191890824580981E-4 4 2.7519514775915473E-4 5 0.0 6 4.8159150857852073E-4 7 1.3759757387957737E-4 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.3759757387957737E-4 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1453514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.46462460137425 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37941652435333 46.189729962494624 2 11.939500065077677 13.721977783355443 3 3.306234809800543 5.6997462656755244 4 1.3986856221744415 3.2149977685437543 5 0.755676849570119 2.1712343240248027 6 0.46212208895660856 1.593340341713663 7 0.3109992400295381 1.2510018205727063 8 0.2170129985702134 0.9976456397164704 9 0.17059596729040874 0.8822909896966493 >10 0.9086525079381211 9.374751620442145 >50 0.07445387943675577 2.965755223677967 >100 0.06809274382210323 7.993824545052093 >500 0.006748948829546079 2.5489158878186964 >1k 0.001807754150771271 1.3947878272155563 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 2443 0.16807543649390375 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 2185 0.15032534946343826 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 1495 0.10285418647498408 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0639636081936602E-4 2 0.0 0.0 0.0 0.0 2.0639636081936602E-4 3 0.0 0.0 0.0 0.0 2.0639636081936602E-4 4 0.0 0.0 0.0 0.0 2.0639636081936602E-4 5 0.0 0.0 0.0 0.0 2.0639636081936602E-4 6 0.0 0.0 0.0 0.0 2.0639636081936602E-4 7 0.0 0.0 0.0 6.879878693978868E-5 2.0639636081936602E-4 8 0.0 0.0 0.0 6.879878693978868E-5 2.0639636081936602E-4 9 0.0 0.0 0.0 6.879878693978868E-5 2.0639636081936602E-4 10 0.0 0.0 0.0 6.879878693978868E-5 2.0639636081936602E-4 11 0.0 0.0 0.0 1.3759757387957737E-4 2.0639636081936602E-4 12 0.0 0.0 0.0 1.3759757387957737E-4 6.191890824580981E-4 13 0.0 0.0 0.0 2.0639636081936602E-4 6.879878693978867E-4 14 6.879878693978868E-5 0.0 0.0 2.0639636081936602E-4 6.879878693978867E-4 15 6.879878693978868E-5 0.0 0.0 2.0639636081936602E-4 8.255854432774641E-4 16 6.879878693978868E-5 0.0 0.0 4.1279272163873205E-4 8.943842302172528E-4 17 6.879878693978868E-5 0.0 0.0 4.1279272163873205E-4 0.00103198180409683 18 6.879878693978868E-5 0.0 0.0 6.191890824580981E-4 0.00103198180409683 19 6.879878693978868E-5 0.0 0.0 6.191890824580981E-4 0.00103198180409683 20 6.879878693978868E-5 0.0 0.0 8.255854432774641E-4 0.0013071769518559848 21 6.879878693978868E-5 0.0 0.0 0.0011695793779764076 0.0014447745257355623 22 6.879878693978868E-5 0.0 0.0 0.0019951648212538716 0.001513573312675351 23 6.879878693978868E-5 0.0 0.0 0.003027146625350702 0.001513573312675351 24 6.879878693978868E-5 0.0 0.0 0.00412792721638732 0.001513573312675351 25 6.879878693978868E-5 0.0 0.0 0.004884713872724996 0.001513573312675351 26 6.879878693978868E-5 0.0 0.0 0.006054293250701404 0.0016511708865549282 27 6.879878693978868E-5 0.0 0.0 0.008462250793594008 0.0016511708865549282 28 6.879878693978868E-5 0.0 0.0 0.01403495253571689 0.0016511708865549282 29 1.3759757387957737E-4 0.0 0.0 0.02504275844608308 0.0016511708865549282 30 1.3759757387957737E-4 0.0 0.0 0.04698957147987567 0.0016511708865549282 31 1.3759757387957737E-4 0.0 0.0 0.08785605092211014 0.0017887684604345057 32 1.3759757387957737E-4 0.0 0.0 0.14062472050492805 0.001926366034314083 33 1.3759757387957737E-4 0.0 0.0 0.1889214689366597 0.001926366034314083 34 1.3759757387957737E-4 0.0 0.0 0.25372992623394064 0.00206396360819366 35 1.3759757387957737E-4 0.0 0.0 0.32369829255170574 0.00206396360819366 36 1.3759757387957737E-4 0.0 0.0 0.42696527174832855 0.00206396360819366 37 1.3759757387957737E-4 0.0 0.0 0.5793545848199605 0.00206396360819366 38 2.0639636081936602E-4 0.0 0.0 0.772885572481586 0.00206396360819366 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATACG 95 6.868795E-7 20.841942 37 TCGTATA 65 4.933083E-4 20.307533 44 CTTACGC 55 0.0044842428 19.999846 3 AGTCGTA 70 8.124829E-4 18.856997 42 CGCAATA 435 0.0 18.712498 36 ATACGAA 435 0.0 18.712498 40 TAATACC 225 0.0 18.577633 4 CGATTAA 60 0.0074131386 18.33319 41 ACGATTA 60 0.0074131386 18.33319 40 GTGTACG 85 1.4277802E-4 18.123116 1 GTACCGT 85 1.4311704E-4 18.117506 6 ATAACGC 425 0.0 18.117504 3 GGCGTTA 330 0.0 17.99986 42 TAACGCC 440 0.0 17.99986 4 GTATAGA 135 5.5088094E-8 17.931337 1 CGTCGTA 335 0.0 17.731205 10 CTAGCGG 465 0.0 17.50524 29 GCGTAAC 215 0.0 17.395212 11 CAATACG 485 0.0 17.236977 38 ACCGTCG 320 0.0 17.187366 8 >>END_MODULE