FastQCFastQC Report
Thu 2 Feb 2017
SRR4062619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26798
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1250.4664527203522651No Hit
TATCAACGCAGAGTACTTTTTTTTT910.339577580416449No Hit
GGTATCAACGCAGAGTACTTTTTTT560.20897081871781475No Hit
GTACATGGAAGCAGTGGTATCAACG400.14926487051272483No Hit
GTACTTTTTTTTTTTTTTTTTTTTT380.1418016269870886No Hit
ACGCAGAGTACTTTTTTTTTTTTTT370.13807000522427046No Hit
TCCATGTACTCTGCGTTGATACCAC320.11941189641017987No Hit
GCAGAGTACTTTTTTTTTTTTTTTT320.11941189641017987No Hit
GAGTACTTTTTTTTTTTTTTTTTTT290.1082170311217255No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG290.1082170311217255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG1100.00642068967.77272753
GTACATG1100.00642068967.77272751
TACATGG1150.0095586267.43478252
Produced by FastQC (version 0.11.5)