##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062619_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26798
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.488805134711544	32.0	32.0	32.0	14.0	32.0
2	28.44540637360997	32.0	32.0	32.0	14.0	32.0
3	28.637323680871706	32.0	32.0	32.0	14.0	32.0
4	28.238301365773566	32.0	32.0	32.0	14.0	32.0
5	28.351294872751698	32.0	32.0	32.0	14.0	32.0
6	31.683707739383536	36.0	32.0	36.0	14.0	36.0
7	31.643965967609525	36.0	32.0	36.0	14.0	36.0
8	31.54351070975446	36.0	32.0	36.0	14.0	36.0
9	31.78517053511456	36.0	32.0	36.0	14.0	36.0
10	30.793305470557506	36.0	32.0	36.0	14.0	36.0
11	32.13101724009255	36.0	32.0	36.0	14.0	36.0
12	31.363459959698485	36.0	32.0	36.0	14.0	36.0
13	31.80427643854019	36.0	32.0	36.0	14.0	36.0
14	31.34114486155683	36.0	32.0	36.0	14.0	36.0
15	31.242891260541832	36.0	32.0	36.0	14.0	36.0
16	31.37528920068662	36.0	32.0	36.0	14.0	36.0
17	31.101462795731024	36.0	32.0	36.0	14.0	36.0
18	31.263751026195983	36.0	32.0	36.0	14.0	36.0
19	31.211769535039927	36.0	32.0	36.0	14.0	36.0
20	31.118814836928127	36.0	32.0	36.0	14.0	36.0
21	31.103104709306663	36.0	32.0	36.0	14.0	36.0
22	30.990633629375328	36.0	32.0	36.0	14.0	36.0
23	30.989029032017314	36.0	32.0	36.0	14.0	36.0
24	30.87484140607508	36.0	32.0	36.0	14.0	36.0
25	30.22628554369729	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	4.0
6	3.0
7	6.0
8	0.0
9	6.0
10	5.0
11	2.0
12	2.0
13	0.0
14	16.0
15	65.0
16	105.0
17	203.0
18	355.0
19	446.0
20	533.0
21	596.0
22	610.0
23	581.0
24	655.0
25	681.0
26	779.0
27	844.0
28	1030.0
29	1189.0
30	1409.0
31	1719.0
32	2110.0
33	2783.0
34	4637.0
35	5421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54724585759069	20.97327959396925	12.248096730855352	25.231377817584715
2	17.51688621860656	20.23734000074635	37.89976489905587	24.34600888159122
3	19.14091655470966	25.19779071503209	28.68711748022093	26.97417525003732
4	12.060020156022546	16.916128550632674	36.96017319249002	34.063678100854766
5	13.780615292712067	37.26478494623656	33.94563918757467	15.0089605734767
6	31.92085122269927	36.89378383423558	17.24099309314915	13.944371849916
7	28.138770632608857	31.996414967510646	21.62596161027709	18.238852789603406
8	27.098195943674597	32.84652448362156	20.67007806372091	19.38520150898293
9	26.55683813366207	14.442078523665433	19.843849228585302	39.15723411408719
10	15.526905829596412	27.05530642750374	31.565769805680123	25.852017937219728
11	35.678786293486795	22.080639736930607	23.395986697059154	18.844587272523448
12	24.197466273029637	24.91871893568519	28.98837774206809	21.895437049217087
13	28.05680119581465	20.78101644245142	26.43124065769806	24.730941704035875
14	22.590890408399655	20.875836042297202	26.428277846280313	30.10499570302283
15	23.997758266392676	28.511115262469644	23.77358490566038	23.7175415654773
16	24.21910028396353	27.2604991780003	24.899118218502466	23.6212823195337
17	22.250868736688712	27.078429174606732	26.648731457609387	24.02197063109517
18	23.322122571001493	25.747384155455904	27.784005979073246	23.146487294469356
19	24.004933288485255	25.72410957880181	27.33116567627163	22.939791456441306
20	24.464626079156858	24.6552304069963	26.987330418208323	23.892813095638523
21	25.200851986099178	25.077538208587125	25.671686409327005	24.049923395986696
22	24.39443779904306	25.470992822966508	26.708283492822964	23.426285885167463
23	22.843474360891015	24.872925698908656	27.877859171774556	24.405740768425773
24	23.70043723607011	26.379909563137637	26.237901266863485	23.681751933928773
25	23.746169369908067	25.27468420659242	26.825622243814934	24.15352417968458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	10.0
3	16.0
4	16.0
5	16.0
6	48.5
7	81.0
8	81.0
9	81.0
10	86.0
11	91.0
12	91.0
13	91.0
14	108.0
15	125.0
16	125.0
17	125.0
18	236.5
19	348.0
20	348.0
21	348.0
22	499.5
23	651.0
24	651.0
25	651.0
26	930.5
27	1210.0
28	1210.0
29	1210.0
30	1445.0
31	1680.0
32	1680.0
33	1680.0
34	1893.0
35	2106.0
36	2106.0
37	2106.0
38	2208.0
39	2310.0
40	2310.0
41	2310.0
42	2521.5
43	2733.0
44	2733.0
45	2733.0
46	3111.5
47	3490.0
48	3490.0
49	3490.0
50	3346.0
51	3202.0
52	3202.0
53	3202.0
54	2876.5
55	2551.0
56	2551.0
57	2551.0
58	2328.0
59	2105.0
60	2105.0
61	2105.0
62	1861.0
63	1617.0
64	1617.0
65	1617.0
66	1344.0
67	1071.0
68	1071.0
69	1071.0
70	830.0
71	589.0
72	589.0
73	589.0
74	487.0
75	385.0
76	385.0
77	385.0
78	306.0
79	227.0
80	227.0
81	227.0
82	166.0
83	105.0
84	105.0
85	105.0
86	74.5
87	44.0
88	44.0
89	44.0
90	32.0
91	20.0
92	20.0
93	20.0
94	12.0
95	4.0
96	4.0
97	4.0
98	18.5
99	33.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007463243525636242
2	0.003731621762818121
3	0.007463243525636242
4	0.026121352339726844
5	0.05224270467945369
6	0.04851108291663557
7	0.07463243525636241
8	0.09329054407045302
9	0.1082170311217255
10	0.1418016269870886
11	0.13807000522427046
12	0.1455332487499067
13	0.1418016269870886
14	0.13060676169863422
15	0.123143518172998
16	0.12687513993581612
17	0.13060676169863422
18	0.1418016269870886
19	0.15299649227554296
20	0.15299649227554296
21	0.13807000522427046
22	0.17165460108963354
23	0.15672811403836107
24	0.1455332487499067
25	0.14926487051272483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	26798.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.0767967758788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.91637959511112	72.68452869617136
2	3.9497900051908825	6.246734830957534
3	1.3637865131423719	3.2353160683633106
4	0.8682931433155585	2.7464736174341366
5	0.42470860270869715	1.6792297932681544
6	0.32560992874333444	1.544891409806702
7	0.2265112547779718	1.2538249123068885
8	0.12741258081260914	0.8060303007687141
9	0.16044547213439667	1.1418762594223448
>10	0.6229059506394223	7.646092992014329
>50	0.009437968949082158	0.5485483991342638
>100	0.004718984474541079	0.4664527203522651
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	125	0.4664527203522651	No Hit
TATCAACGCAGAGTACTTTTTTTTT	91	0.339577580416449	No Hit
GGTATCAACGCAGAGTACTTTTTTT	56	0.20897081871781475	No Hit
GTACATGGAAGCAGTGGTATCAACG	40	0.14926487051272483	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	38	0.1418016269870886	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	37	0.13807000522427046	No Hit
TCCATGTACTCTGCGTTGATACCAC	32	0.11941189641017987	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	32	0.11941189641017987	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	29	0.1082170311217255	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	29	0.1082170311217255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	110	0.0064206896	7.7727275	3
GTACATG	110	0.0064206896	7.7727275	1
TACATGG	115	0.009558626	7.4347825	2
>>END_MODULE