##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062619_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26798
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.175908649899245	32.0	32.0	32.0	21.0	32.0
2	30.0970594820509	32.0	32.0	32.0	21.0	32.0
3	30.84021195611613	32.0	32.0	32.0	32.0	32.0
4	31.20658258078961	32.0	32.0	32.0	32.0	32.0
5	31.00100753787596	32.0	32.0	32.0	32.0	32.0
6	34.535151877005745	36.0	36.0	36.0	32.0	36.0
7	34.54171953130831	36.0	36.0	36.0	32.0	36.0
8	34.44219717889395	36.0	36.0	36.0	32.0	36.0
9	34.7390103739085	36.0	36.0	36.0	32.0	36.0
10	34.40592581535935	36.0	36.0	36.0	32.0	36.0
11	34.876520635868346	36.0	36.0	36.0	32.0	36.0
12	34.600194044331666	36.0	36.0	36.0	32.0	36.0
13	34.74475707142324	36.0	36.0	36.0	32.0	36.0
14	34.670945592954695	36.0	36.0	36.0	32.0	36.0
15	34.58138667064706	36.0	36.0	36.0	32.0	36.0
16	34.59485782521084	36.0	36.0	36.0	32.0	36.0
17	34.56377341592656	36.0	36.0	36.0	32.0	36.0
18	34.569221583700276	36.0	36.0	36.0	32.0	36.0
19	34.599783565937756	36.0	36.0	36.0	32.0	36.0
20	34.56851257556534	36.0	36.0	36.0	32.0	36.0
21	34.60191059034256	36.0	36.0	36.0	32.0	36.0
22	34.58672288976789	36.0	36.0	36.0	32.0	36.0
23	34.538398387939395	36.0	36.0	36.0	32.0	36.0
24	34.47421449361892	36.0	36.0	36.0	32.0	36.0
25	34.43245764609299	36.0	36.0	36.0	32.0	36.0
26	34.384170460482125	36.0	36.0	36.0	32.0	36.0
27	34.3336816180312	36.0	36.0	36.0	32.0	36.0
28	34.32480035823569	36.0	36.0	36.0	32.0	36.0
29	34.2870736622136	36.0	36.0	36.0	32.0	36.0
30	34.235092171057545	36.0	36.0	36.0	32.0	36.0
31	34.292148667811034	36.0	36.0	36.0	32.0	36.0
32	34.18381968803642	36.0	36.0	36.0	32.0	36.0
33	34.195798193895065	36.0	36.0	36.0	32.0	36.0
34	34.11896410179864	36.0	36.0	36.0	32.0	36.0
35	34.047540861258305	36.0	36.0	36.0	32.0	36.0
36	34.06418389432047	36.0	36.0	36.0	32.0	36.0
37	34.03115904171953	36.0	36.0	36.0	32.0	36.0
38	34.024889917158	36.0	36.0	36.0	32.0	36.0
39	34.00044779461154	36.0	36.0	36.0	32.0	36.0
40	33.94327934920516	36.0	36.0	36.0	32.0	36.0
41	33.88301365773565	36.0	36.0	36.0	32.0	36.0
42	33.782035972833796	36.0	36.0	36.0	32.0	36.0
43	33.9169714157773	36.0	36.0	36.0	32.0	36.0
44	33.819911933726395	36.0	36.0	36.0	32.0	36.0
45	33.794574221956864	36.0	36.0	36.0	32.0	36.0
46	33.81297111724756	36.0	36.0	36.0	32.0	36.0
47	33.711433689081275	36.0	36.0	36.0	27.0	36.0
48	33.662810657511756	36.0	36.0	36.0	27.0	36.0
49	33.641615045898945	36.0	36.0	36.0	27.0	36.0
50	32.99567131875513	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	1.0
22	8.0
23	25.0
24	35.0
25	83.0
26	170.0
27	269.0
28	448.0
29	647.0
30	960.0
31	1460.0
32	2177.0
33	3415.0
34	6094.0
35	11004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.439170025376924	20.014181221077774	12.893715479922378	24.652933273622928
2	17.75505634748862	21.28517053511456	36.11090379879096	24.848869318605864
3	19.695499664154042	24.572729308157328	29.543249496231063	26.18852153145757
4	14.015971341144862	16.99380550787372	37.61474736920666	31.37547578177476
5	16.870661989700725	34.61079185013807	33.40920964251063	15.10933651765057
6	32.8867825957161	36.857228151354576	16.628106575117545	13.627882677811776
7	28.091648630494813	31.838196880364205	21.012762146428837	19.05739234271214
8	26.960967236360922	33.84954101052317	19.572356145981043	19.617135607134863
9	27.158743189790286	14.202552429285767	19.06112396447496	39.57758041644899
10	15.739980595566832	25.87133368161803	32.39794014478692	25.990745578028214
11	36.37958056571386	21.70684379431301	23.102470333606988	18.811105306366148
12	24.277931188894694	24.26300470184342	28.617807299052167	22.841256810209718
13	28.300619449212626	20.74781700126875	25.281737443092766	25.66982610642585
14	23.180834390626163	20.796328084185387	25.464586909470853	30.55825061571759
15	24.554071199343237	27.927457272930816	22.527800582132997	24.990670945592957
16	24.837674453317412	27.214717516232557	23.990596313157695	23.957011717292335
17	22.87484140607508	27.099037241585194	25.729532054630944	24.296589297708785
18	23.82267333383088	26.005672065079484	26.893798044630195	23.277856556459437
19	24.550339577580417	26.63258452123293	25.662362862900217	23.154713038286438
20	24.919770132099412	26.184789909694757	25.25561609075304	23.639823867452794
21	25.807896111650123	25.319053660720947	24.792894992163596	24.080155235465334
22	24.632435256362413	25.860138816329574	25.13993581610568	24.36749011120233
23	23.882379282035973	26.13627882677812	25.949697738637212	24.031644152548697
24	23.755504142100158	26.45346667661766	25.4944398835734	24.296589297708785
25	24.098813344279424	25.546682588252857	25.639973132323306	24.714530935144413
26	23.55026494514516	26.378834241361293	25.975819090976938	24.095081722516607
27	24.669751473990598	26.39002910664975	25.00186581088141	23.938353608478245
28	23.33009926113889	26.315396671393387	26.091499365624298	24.26300470184342
29	23.98313306963206	26.419882080752295	25.222031494887677	24.374953354727964
30	23.76669900738861	26.86394507052765	25.710873945816854	23.658481976266884
31	24.292857675945967	26.199716396746027	24.882453914471228	24.62497201283678
32	23.51294872751698	27.158743189790286	25.333980147772223	23.994327934920516
33	24.080155235465334	26.259422344951115	25.55041421001567	24.11000820956788
34	24.04657063959997	25.931039629823122	25.99820882155385	24.02418090902306
35	24.70706769161878	26.1735950444063	25.32651690424659	23.792820359728335
36	23.9495484737667	26.386297484886935	25.210836629599225	24.453317411747143
37	24.938428240913503	26.069109635047393	25.19964176431077	23.792820359728335
38	23.740577655048885	25.87506530338085	25.972087469214124	24.412269572356145
39	24.16971415777297	26.442271811329203	24.792894992163596	24.595119038734232
40	24.826479588028956	26.274348832002385	25.62131502350922	23.277856556459437
41	23.774162250914248	26.054183147996117	25.983282334502572	24.18837226658706
42	24.356295245913877	26.5654153295022	25.800432868124485	23.277856556459437
43	23.42712142697216	26.01686693036794	26.62885289947011	23.927158743189793
44	23.591312784536157	25.804164489887306	26.27061721023957	24.333905515336966
45	23.647287110978432	26.199716396746027	25.766848272259125	24.38614822001642
46	23.662213598029705	25.333980147772223	25.972087469214124	25.03171878498395
47	23.673408463318157	25.710873945816854	26.229569370848573	24.38614822001642
48	24.26300470184342	26.296738562579296	25.427270691842672	24.012986043734607
49	23.039032763639078	26.09523098738712	26.434808567803568	24.430927681170235
50	23.11366519889544	26.755728039405923	25.725800432868123	24.40480632883051
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	6.5
4	9.0
5	12.0
6	15.0
7	19.5
8	24.0
9	23.0
10	22.0
11	23.5
12	25.0
13	33.0
14	41.0
15	53.5
16	66.0
17	78.0
18	90.0
19	100.5
20	111.0
21	144.0
22	177.0
23	223.5
24	270.0
25	306.0
26	342.0
27	397.0
28	452.0
29	508.0
30	564.0
31	615.5
32	667.0
33	725.0
34	783.0
35	925.0
36	1067.0
37	1141.0
38	1215.0
39	1264.0
40	1313.0
41	1416.0
42	1519.0
43	1456.0
44	1393.0
45	1509.5
46	1626.0
47	1741.0
48	1856.0
49	1805.0
50	1754.0
51	1684.0
52	1614.0
53	1581.5
54	1549.0
55	1569.5
56	1590.0
57	1528.5
58	1467.0
59	1345.0
60	1223.0
61	1095.5
62	968.0
63	856.5
64	745.0
65	649.5
66	554.0
67	477.5
68	401.0
69	392.5
70	384.0
71	289.5
72	195.0
73	192.5
74	190.0
75	146.0
76	102.0
77	108.0
78	114.0
79	103.0
80	92.0
81	75.0
82	58.0
83	45.5
84	33.0
85	32.0
86	31.0
87	22.5
88	14.0
89	9.0
90	4.0
91	6.0
92	8.0
93	4.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	25.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007463243525636242
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	26798.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.49511157549071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.2341120876209	78.77826703485334
2	3.599346376363556	6.082543473393537
3	1.165923243386477	2.9554444361519514
4	0.5741288698494016	1.9404433166654227
5	0.3709755774411518	1.5672811403836109
6	0.27381530715894536	1.388163295768341
7	0.17223866095482046	1.018732741249347
8	0.14574040542330963	0.985148145383984
9	0.08832751843836947	0.6716919173072617
>10	0.37539195336307024	4.612284498843197
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	47	0.17538622285245167	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	36	0.13433838346145233	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	28	0.10448540935890738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.003731621762818121
2	0.0	0.0	0.0	0.0	0.003731621762818121
3	0.0	0.0	0.0	0.0	0.003731621762818121
4	0.0	0.0	0.0	0.0	0.003731621762818121
5	0.0	0.0	0.0	0.0	0.003731621762818121
6	0.0	0.0	0.0	0.0	0.003731621762818121
7	0.0	0.0	0.0	0.0	0.003731621762818121
8	0.0	0.0	0.0	0.0	0.003731621762818121
9	0.0	0.0	0.0	0.0	0.003731621762818121
10	0.0	0.0	0.0	0.0	0.003731621762818121
11	0.0	0.0	0.0	0.0	0.003731621762818121
12	0.0	0.0	0.0	0.0	0.003731621762818121
13	0.0	0.0	0.0	0.0	0.003731621762818121
14	0.0	0.0	0.0	0.0	0.003731621762818121
15	0.0	0.0	0.0	0.0	0.003731621762818121
16	0.0	0.0	0.0	0.0	0.003731621762818121
17	0.0	0.0	0.0	0.0	0.003731621762818121
18	0.0	0.0	0.0	0.0	0.003731621762818121
19	0.0	0.0	0.0	0.0	0.003731621762818121
20	0.0	0.0	0.0	0.0	0.003731621762818121
21	0.0	0.0	0.0	0.0	0.003731621762818121
22	0.0	0.0	0.0	0.0	0.003731621762818121
23	0.0	0.0	0.0	0.0	0.003731621762818121
24	0.0	0.0	0.0	0.0	0.003731621762818121
25	0.0	0.0	0.0	0.007463243525636242	0.003731621762818121
26	0.0	0.0	0.0	0.011194865288454363	0.003731621762818121
27	0.0	0.0	0.0	0.011194865288454363	0.003731621762818121
28	0.0	0.0	0.0	0.014926487051272483	0.003731621762818121
29	0.0	0.0	0.0	0.022389730576908726	0.003731621762818121
30	0.0	0.0	0.0	0.055974326442271814	0.003731621762818121
31	0.0	0.0	0.0	0.11941189641017987	0.003731621762818121
32	0.0	0.0	0.0	0.19404433166654228	0.003731621762818121
33	0.0	0.0	0.0	0.26121352339726844	0.003731621762818121
34	0.0	0.0	0.0	0.34330920217926714	0.003731621762818121
35	0.0	0.0	0.0	0.43659974624972014	0.003731621762818121
36	0.0	0.0	0.0	0.5709381297111725	0.003731621762818121
37	0.0	0.0	0.0	0.742592730800806	0.003731621762818121
38	0.0	0.0	0.0	0.9664900365698933	0.003731621762818121
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGAGA	25	0.0023120327	35.2	6
>>END_MODULE