FastQCFastQC Report
Thu 2 Feb 2017
SRR4062618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772216
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42970.24246480113033625No Hit
TATCAACGCAGAGTACTTTTTTTTT29970.16911031160987147No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29710.1676432218194622No Hit
GTACATGGAAGCAGTGGTATCAACG22120.12481548524559084No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21630.12205058525597332No Hit
TCCATGTACTCTGCGTTGATACCAC18680.10540475878786784No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18540.10461478736226283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT400.00527516314.2517610
TACCGTT400.00527615214.2513577
CGCATCG2850.013.33497913
AAAGCGT1051.990702E-812.6682310
TCGCCAG3150.012.36800517
CGCCAGT3150.012.36800518
ATCGCCA3150.012.36695616
CGTTCAA1001.440003E-712.35187414
TTGTCGA701.0900963E-412.2154487
GCATCGC3150.012.06532314
CGTCTTA1501.4551915E-1112.035515
CGACCAT2450.012.02189310
CGACAAT802.859955E-511.87780917
CCGTCGT2250.011.8236839
ACGGTAT2500.011.7814549
ATAAGGC1052.734123E-711.7573753
CGTTATT1552.5465852E-1111.6383752
CGCAAGA2800.011.5289932
CGAACGA1501.7644197E-1011.4017316
GTCGATA752.0717476E-411.4014089
Produced by FastQC (version 0.11.5)