##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062618_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1772216
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138229200052365	32.0	32.0	32.0	32.0	32.0
2	30.782044626614365	32.0	32.0	32.0	32.0	32.0
3	30.76790075250421	32.0	32.0	32.0	32.0	32.0
4	30.84328546858848	32.0	32.0	32.0	32.0	32.0
5	30.63536893922637	32.0	32.0	32.0	32.0	32.0
6	34.27840398687293	36.0	36.0	36.0	32.0	36.0
7	34.153279284240746	36.0	36.0	36.0	32.0	36.0
8	34.14531129388291	36.0	36.0	36.0	32.0	36.0
9	34.31530298789764	36.0	36.0	36.0	32.0	36.0
10	33.97293783601999	36.0	36.0	36.0	32.0	36.0
11	34.31922519602577	36.0	36.0	36.0	32.0	36.0
12	34.072218623463506	36.0	36.0	36.0	32.0	36.0
13	34.211154847941785	36.0	36.0	36.0	32.0	36.0
14	34.102776975267126	36.0	36.0	36.0	32.0	36.0
15	34.04686054070159	36.0	36.0	36.0	32.0	36.0
16	34.05365655202301	36.0	36.0	36.0	32.0	36.0
17	33.97475477029888	36.0	36.0	36.0	32.0	36.0
18	33.99306856500562	36.0	36.0	36.0	32.0	36.0
19	33.976775404352516	36.0	36.0	36.0	32.0	36.0
20	33.95701257634509	36.0	36.0	36.0	32.0	36.0
21	33.93814580164043	36.0	36.0	36.0	32.0	36.0
22	33.89565944557548	36.0	36.0	36.0	32.0	36.0
23	33.871335096850494	36.0	36.0	36.0	32.0	36.0
24	33.83004272616882	36.0	36.0	36.0	32.0	36.0
25	33.38985879825032	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	9.0
4	72.0
5	170.0
6	266.0
7	79.0
8	276.0
9	334.0
10	193.0
11	73.0
12	149.0
13	113.0
14	370.0
15	436.0
16	648.0
17	863.0
18	1196.0
19	1691.0
20	2489.0
21	3832.0
22	5512.0
23	8147.0
24	11470.0
25	15847.0
26	22048.0
27	28109.0
28	37946.0
29	50080.0
30	64360.0
31	88088.0
32	126360.0
33	182747.0
34	403582.0
35	714660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19557309683456	17.76037569537164	11.202410283092016	25.841640924701792
2	16.736054604488753	20.185287801002392	37.027709656340676	26.05094793816818
3	19.051133773028706	24.067960923992292	28.476651904819946	28.404253398159057
4	12.632142703961968	15.392833198265413	35.95940738667275	36.015616711099874
5	14.613504959638203	36.61862913827662	33.44934892224604	15.318516979839139
6	33.843313809893445	35.39363488815186	17.103380142544633	13.659671159410063
7	29.980491743575122	30.79933033886773	20.897153940187856	18.323023977369292
8	28.11589308224408	32.704311228984906	19.824898680245244	19.35489700852577
9	27.40184377919383	14.351700711778747	18.974591272266384	39.27186423676104
10	15.94177108066868	26.676315746360267	31.851001939419827	25.53091123355123
11	36.92475178835544	21.447045357733234	22.462603004866498	19.16559984904484
12	24.604779541595686	24.077216237671873	28.770648111264606	22.54735610946783
13	29.396318193502463	19.70718424003774	25.381810169740486	25.51468739671931
14	23.295258089812226	20.065943353800332	25.34655288914601	31.29224566724143
15	24.918878306160956	27.36738636748805	22.731530126879754	24.98220519947124
16	25.38961435127544	26.02473602031847	24.17620036470208	24.40944926370401
17	23.653467628760673	26.08540655854032	25.55925668972468	24.701869122974326
18	24.491314505580807	25.050440560369058	26.76307485430009	23.695170079750046
19	25.238971273726214	25.45141529529154	25.704992095500366	23.60462133548188
20	25.38720591982274	24.587116882940794	25.57612681654165	24.449550380694816
21	26.26556030664087	24.486747184002603	24.816316060677284	24.431376448679238
22	25.360455614540516	24.997824325286537	25.103217399068473	24.538502661104474
23	24.125634273961147	24.778442031032807	25.787234283002025	25.30868941200402
24	24.37145341633156	25.515061556516223	25.601453693193214	24.512031333959
25	24.46520415779366	25.062701955668203	25.70969460658655	24.76239927995159
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	383.0
1	383.0
2	818.0
3	1253.0
4	1253.0
5	1253.0
6	3240.0
7	5227.0
8	5227.0
9	5227.0
10	5427.0
11	5627.0
12	5627.0
13	5627.0
14	6022.0
15	6417.0
16	6417.0
17	6417.0
18	10184.5
19	13952.0
20	13952.0
21	13952.0
22	21999.5
23	30047.0
24	30047.0
25	30047.0
26	43451.5
27	56856.0
28	56856.0
29	56856.0
30	69040.0
31	81224.0
32	81224.0
33	81224.0
34	98451.0
35	115678.0
36	115678.0
37	115678.0
38	129944.5
39	144211.0
40	144211.0
41	144211.0
42	163035.5
43	181860.0
44	181860.0
45	181860.0
46	207773.0
47	233686.0
48	233686.0
49	233686.0
50	233832.0
51	233978.0
52	233978.0
53	233978.0
54	215714.5
55	197451.0
56	197451.0
57	197451.0
58	180053.5
59	162656.0
60	162656.0
61	162656.0
62	144068.0
63	125480.0
64	125480.0
65	125480.0
66	104033.5
67	82587.0
68	82587.0
69	82587.0
70	62889.0
71	43191.0
72	43191.0
73	43191.0
74	33755.5
75	24320.0
76	24320.0
77	24320.0
78	19625.0
79	14930.0
80	14930.0
81	14930.0
82	10578.5
83	6227.0
84	6227.0
85	6227.0
86	4560.0
87	2893.0
88	2893.0
89	2893.0
90	1975.0
91	1057.0
92	1057.0
93	1057.0
94	665.5
95	274.0
96	274.0
97	274.0
98	512.5
99	751.0
100	751.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008012567316850768
2	0.003047032641619306
3	0.004514122432028601
4	0.015122310147295816
5	0.03470231619621987
6	0.047454712066700676
7	0.06607546709881865
8	0.08937962415416631
9	0.09716648534941565
10	0.11843928731035043
11	0.12154274648237008
12	0.13435156888325125
13	0.1219377321951726
14	0.12481548524559084
15	0.1148844158951279
16	0.11307876692231646
17	0.12346124851598224
18	0.13040171175522622
19	0.13192522807603588
20	0.12205058525597332
21	0.1313609627720323
22	0.14953030556094743
23	0.13971208927128523
24	0.13423871582245053
25	0.13175594848483482
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1772216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68551695218871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02277645557608	42.42378605014849
2	13.031446413227341	13.991998746577107
3	3.6676927438099067	5.9070594291967895
4	1.480784127948667	3.1798664561408065
5	0.7441205547066561	1.997424832708813
6	0.4497926455692321	1.4488410419206093
7	0.2664940777214329	1.001481062902032
8	0.20204953920958277	0.8677707169934379
9	0.15412393942518704	0.7446801026474086
>10	0.7874159818398644	7.80395077263548
>50	0.08683610721829828	3.2801267983551328
>100	0.09148436529665277	10.38941223236887
>500	0.011606586828313423	4.047354192289929
>1k	0.003376461622782086	2.9162475651150475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4297	0.24246480113033625	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2997	0.16911031160987147	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2971	0.1676432218194622	No Hit
GTACATGGAAGCAGTGGTATCAACG	2212	0.12481548524559084	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2163	0.12205058525597332	No Hit
TCCATGTACTCTGCGTTGATACCAC	1868	0.10540475878786784	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1854	0.10461478736226283	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1285306080071503E-4
2	0.0	0.0	0.0	0.0	1.1285306080071503E-4
3	0.0	0.0	0.0	0.0	1.1285306080071503E-4
4	0.0	0.0	0.0	5.642653040035752E-5	1.1285306080071503E-4
5	0.0	0.0	0.0	5.642653040035752E-5	1.1285306080071503E-4
6	1.1285306080071503E-4	0.0	0.0	5.642653040035752E-5	2.2570612160143007E-4
7	1.1285306080071503E-4	0.0	0.0	5.642653040035752E-5	2.2570612160143007E-4
8	1.1285306080071503E-4	0.0	0.0	5.642653040035752E-5	2.821326520017876E-4
9	1.1285306080071503E-4	0.0	0.0	1.1285306080071503E-4	2.821326520017876E-4
10	1.1285306080071503E-4	0.0	5.642653040035752E-5	1.1285306080071503E-4	2.821326520017876E-4
11	1.1285306080071503E-4	0.0	5.642653040035752E-5	1.1285306080071503E-4	2.821326520017876E-4
12	1.1285306080071503E-4	0.0	5.642653040035752E-5	1.1285306080071503E-4	4.5141224320286013E-4
13	1.1285306080071503E-4	0.0	5.642653040035752E-5	1.1285306080071503E-4	4.5141224320286013E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCAT	40	0.005275163	14.25176	10
TACCGTT	40	0.005276152	14.251357	7
CGCATCG	285	0.0	13.334979	13
AAAGCGT	105	1.990702E-8	12.66823	10
TCGCCAG	315	0.0	12.368005	17
CGCCAGT	315	0.0	12.368005	18
ATCGCCA	315	0.0	12.366956	16
CGTTCAA	100	1.440003E-7	12.351874	14
TTGTCGA	70	1.0900963E-4	12.215448	7
GCATCGC	315	0.0	12.065323	14
CGTCTTA	150	1.4551915E-11	12.0355	15
CGACCAT	245	0.0	12.021893	10
CGACAAT	80	2.859955E-5	11.877809	17
CCGTCGT	225	0.0	11.823683	9
ACGGTAT	250	0.0	11.781454	9
ATAAGGC	105	2.734123E-7	11.757375	3
CGTTATT	155	2.5465852E-11	11.638375	2
CGCAAGA	280	0.0	11.528993	2
CGAACGA	150	1.7644197E-10	11.40173	16
GTCGATA	75	2.0717476E-4	11.401408	9
>>END_MODULE