FastQCFastQC Report
Thu 2 Feb 2017
SRR4062618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772216
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA4750.021.3051836
CGTCTTA2700.020.37029315
GCCGGTC5750.019.512978
CGGTCCA5800.018.96544310
TCTAGCG5150.018.79604528
CTAGCGG5150.018.79604529
CCGGTCC5850.018.4272799
CGCCGGT6000.018.3332637
TATACCG600.007413597318.3332635
TACGTAC600.007413597318.3332633
GGTACGA851.431351E-418.11757942
CAATACG5650.017.91143638
ATACGAA5550.017.8377740
TAGCGGC5500.017.59993430
TTAACGG2750.017.59993435
TAATACC1509.604264E-917.5999324
ATCGGTT750.001291225417.59993222
TACCGTC4100.017.1706667
ATACCGT4150.016.963796
GTCCTAA4950.016.8912071