FastQCFastQC Report
Thu 2 Feb 2017
SRR4062617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252903
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG64280.513048496172489No Hit
TCCATGTACTCTGCGTTGATACCAC53280.42525239384054475No Hit
GTATCAACGCAGAGTACTTTTTTTT28270.22563598299309684No Hit
GAGTACATGGAAGCAGTGGTATCAA28080.2241195048619087No Hit
CATGTACTCTGCGTTGATACCACTG24100.19235327874544159No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20370.16258241859106412No Hit
TATCAACGCAGAGTACTTTTTTTTT19670.15699539389721312No Hit
GCTTCCATGTACTCTGCGTTGATAC17800.14207005650078258No Hit
CCCATGTACTCTGCGTTGATACCAC16710.13337026090607174No Hit
GCGTTGATACCACTGCTTCCATGTA15320.12227602615685332No Hit
GTACATGGGAAGCAGTGGTATCAAC15260.1217971383259518No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14570.1162899282705844No Hit
CATGGAAGCAGTGGTATCAACGCAG14470.11549178188574855No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12540.10008755665841651No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12530.10000774201993291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA801.2855708E-714.25194716
ATTGGAC400.005291758614.244553
AATATTG500.001501330913.2975675
CCGACCA1650.013.2447629
CAAGACG1650.013.2384154
TCGCGTA905.390575E-712.6689049
ACGAACG905.3931944E-712.66839815
TAACGAA905.398415E-712.66738613
GGCTTAC905.4351585E-712.6603041
CGACCAT1457.2759576E-1212.45047610
CGCGCTA701.0891823E-412.21595616
CGGCGTC1251.4078978E-912.16069114
TCGCCAG2200.012.09256317
CGAAATC550.003066567212.09159713
GGTATAT550.003079437712.0848361
GCACTAG550.003079437712.0848361
CGCGTAA951.0370095E-612.0021210
GGGTCGC951.0380099E-612.0011616
AAGACGG1900.011.9978065
CCCTAGA802.8800867E-511.8690351
Produced by FastQC (version 0.11.5)