##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062617_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1252903
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.100135445441506	32.0	32.0	32.0	32.0	32.0
2	30.687477801553673	32.0	32.0	32.0	32.0	32.0
3	30.674421722990527	32.0	32.0	32.0	32.0	32.0
4	30.746332317825082	32.0	32.0	32.0	32.0	32.0
5	30.504327948771774	32.0	32.0	32.0	32.0	32.0
6	34.15864516247467	36.0	36.0	36.0	32.0	36.0
7	34.03469063447051	36.0	36.0	36.0	32.0	36.0
8	34.00443929019246	36.0	36.0	36.0	32.0	36.0
9	34.20968582563854	36.0	36.0	36.0	32.0	36.0
10	33.80428492868163	36.0	36.0	36.0	32.0	36.0
11	34.21411713436714	36.0	36.0	36.0	32.0	36.0
12	33.933512809850406	36.0	36.0	36.0	32.0	36.0
13	34.081157120702876	36.0	36.0	36.0	32.0	36.0
14	33.9588771038141	36.0	36.0	36.0	32.0	36.0
15	33.886597765349755	36.0	36.0	36.0	32.0	36.0
16	33.89060446020163	36.0	36.0	36.0	32.0	36.0
17	33.80772493960027	36.0	36.0	36.0	32.0	36.0
18	33.820463355902255	36.0	36.0	36.0	32.0	36.0
19	33.81109950251536	36.0	36.0	36.0	32.0	36.0
20	33.79885912955751	36.0	36.0	36.0	32.0	36.0
21	33.77080987115523	36.0	36.0	36.0	32.0	36.0
22	33.718928759848126	36.0	36.0	36.0	27.0	36.0
23	33.65095701742274	36.0	36.0	36.0	27.0	36.0
24	33.63476422356719	36.0	36.0	36.0	27.0	36.0
25	33.13893174491561	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	45.0
5	122.0
6	209.0
7	56.0
8	185.0
9	187.0
10	163.0
11	59.0
12	96.0
13	58.0
14	268.0
15	332.0
16	502.0
17	624.0
18	917.0
19	1361.0
20	2025.0
21	3009.0
22	4569.0
23	6656.0
24	9281.0
25	12890.0
26	17644.0
27	22069.0
28	29352.0
29	38416.0
30	49024.0
31	66627.0
32	94237.0
33	133592.0
34	283174.0
35	475134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.49299812900301	16.80959891699287	10.884189756131825	25.813213197872297
2	16.761542771669983	19.87896512145791	36.51750951022539	26.84198259664672
3	19.33907068888176	23.91825944532511	28.01510485759577	28.727565008197363
4	12.670878851135333	15.206491500460997	35.25231197838295	36.87031767002071
5	14.536692237431255	36.60065853767589	33.56506173391501	15.297587490977843
6	34.76348240900666	34.47330029058138	16.962877610061987	13.800339690349967
7	30.268290423265043	30.33106616531265	20.90136700967034	18.49927640175197
8	28.63522201487768	32.49979230041668	19.66212070860707	19.20286497609857
9	27.26096870011776	14.408906940811603	18.34571917507258	39.98440518399806
10	15.697466286945321	26.85513694580021	31.986649023048574	25.460747744205893
11	37.60383246165341	21.13575432610285	21.493349528333926	19.767063683909814
12	24.14924070602113	23.851069372985087	28.84741715376242	23.152272767231363
13	30.04171361446072	19.362708667961225	24.955689273527838	25.639888444050218
14	23.705511510249224	19.451669899141976	24.87999085796847	31.96282773264033
15	25.56687109211131	26.616033080964463	22.791985457160553	25.02511036976368
16	26.133324597268754	25.448395826824566	23.89739811764443	24.520881458262252
17	24.145150592541096	25.57915597855185	25.682481081037885	24.59321234786917
18	25.289914546034296	24.311984892717035	26.51742124497629	23.880679316272374
19	25.09476409873208	26.020866882714692	25.208728766488314	23.675640252064913
20	25.309833234643786	24.030385192892115	25.887666523626756	24.772115048837342
21	26.161858654182723	24.794416109785395	24.150213295783473	24.893511940248413
22	25.21573912431817	24.782902014950665	24.798089281946016	25.203269578785147
23	23.941086391493524	24.61340741759032	25.13898790589424	26.306518285021912
24	24.222633013731144	25.79786660145152	24.99330665586141	24.98619372895593
25	25.096378532680795	24.653245063597044	24.68712939017802	25.56324701354414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	230.0
1	230.0
2	488.5
3	747.0
4	747.0
5	747.0
6	1991.5
7	3236.0
8	3236.0
9	3236.0
10	3388.5
11	3541.0
12	3541.0
13	3541.0
14	3706.5
15	3872.0
16	3872.0
17	3872.0
18	6094.5
19	8317.0
20	8317.0
21	8317.0
22	12968.0
23	17619.0
24	17619.0
25	17619.0
26	25593.0
27	33567.0
28	33567.0
29	33567.0
30	41164.5
31	48762.0
32	48762.0
33	48762.0
34	60712.0
35	72662.0
36	72662.0
37	72662.0
38	83829.0
39	94996.0
40	94996.0
41	94996.0
42	111790.5
43	128585.0
44	128585.0
45	128585.0
46	156318.0
47	184051.0
48	184051.0
49	184051.0
50	177736.0
51	171421.0
52	171421.0
53	171421.0
54	157900.0
55	144379.0
56	144379.0
57	144379.0
58	132921.5
59	121464.0
60	121464.0
61	121464.0
62	106738.0
63	92012.0
64	92012.0
65	92012.0
66	75728.5
67	59445.0
68	59445.0
69	59445.0
70	44813.0
71	30181.0
72	30181.0
73	30181.0
74	23309.5
75	16438.0
76	16438.0
77	16438.0
78	13255.5
79	10073.0
80	10073.0
81	10073.0
82	7045.5
83	4018.0
84	4018.0
85	4018.0
86	2979.0
87	1940.0
88	1940.0
89	1940.0
90	1304.5
91	669.0
92	669.0
93	669.0
94	395.0
95	121.0
96	121.0
97	121.0
98	339.0
99	557.0
100	557.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007582390655940643
2	0.0032724001778270144
3	0.004868692947498729
4	0.015005152034914118
5	0.03440010918642544
6	0.04677137815138123
7	0.0660067060259254
8	0.08739702913952636
9	0.09617663937272079
10	0.11644955754755157
11	0.11876418206357554
12	0.12842175332008943
13	0.1207595480256652
14	0.12291454326472202
15	0.11397530375456041
16	0.11062308893824982
17	0.1207595480256652
18	0.12658601663496694
19	0.13073637783611342
20	0.12004121627931294
21	0.1268254605504177
22	0.1480561543870515
23	0.13688210499934952
24	0.1324922998827523
25	0.12666583127345055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1252903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.8202555213824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.371124489749	44.86336705558624
2	12.175974976723214	13.593320688452959
3	3.4230360739657635	5.732242449230355
4	1.3764025149240158	3.0732456033332767
5	0.6791870997010231	1.895619872606887
6	0.43198289848510385	1.4468037464583539
7	0.27818295454780384	1.0869770523186062
8	0.19610190845025552	0.8757166910339201
9	0.14073390363662733	0.7070222215366331
>10	0.7621785707904535	7.822169862426557
>50	0.07397990484831272	2.927180866702734
>100	0.07994026827173538	9.319536595317633
>500	0.007306361939080895	2.7681343356272623
>1k	0.003581549970137694	2.948544881956169
>5k	2.865239976110155E-4	0.9401180774125119
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6428	0.513048496172489	No Hit
TCCATGTACTCTGCGTTGATACCAC	5328	0.42525239384054475	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2827	0.22563598299309684	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2808	0.2241195048619087	No Hit
CATGTACTCTGCGTTGATACCACTG	2410	0.19235327874544159	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2037	0.16258241859106412	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1967	0.15699539389721312	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1780	0.14207005650078258	No Hit
CCCATGTACTCTGCGTTGATACCAC	1671	0.13337026090607174	No Hit
GCGTTGATACCACTGCTTCCATGTA	1532	0.12227602615685332	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1526	0.1217971383259518	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1457	0.1162899282705844	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1447	0.11549178188574855	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1254	0.10008755665841651	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1253	0.10000774201993291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5962927696717145E-4	0.0
5	0.0	0.0	0.0	1.5962927696717145E-4	0.0
6	0.0	0.0	0.0	1.5962927696717145E-4	0.0
7	0.0	0.0	0.0	1.5962927696717145E-4	0.0
8	0.0	0.0	0.0	1.5962927696717145E-4	0.0
9	0.0	0.0	0.0	1.5962927696717145E-4	0.0
10	0.0	0.0	0.0	1.5962927696717145E-4	0.0
11	0.0	0.0	0.0	1.5962927696717145E-4	0.0
12	0.0	0.0	0.0	1.5962927696717145E-4	7.981463848358572E-5
13	0.0	0.0	0.0	1.5962927696717145E-4	7.981463848358572E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	80	1.2855708E-7	14.251947	16
ATTGGAC	40	0.0052917586	14.24455	3
AATATTG	50	0.0015013309	13.297567	5
CCGACCA	165	0.0	13.244762	9
CAAGACG	165	0.0	13.238415	4
TCGCGTA	90	5.390575E-7	12.668904	9
ACGAACG	90	5.3931944E-7	12.668398	15
TAACGAA	90	5.398415E-7	12.667386	13
GGCTTAC	90	5.4351585E-7	12.660304	1
CGACCAT	145	7.2759576E-12	12.450476	10
CGCGCTA	70	1.0891823E-4	12.215956	16
CGGCGTC	125	1.4078978E-9	12.160691	14
TCGCCAG	220	0.0	12.092563	17
CGAAATC	55	0.0030665672	12.091597	13
GGTATAT	55	0.0030794377	12.084836	1
GCACTAG	55	0.0030794377	12.084836	1
CGCGTAA	95	1.0370095E-6	12.00212	10
GGGTCGC	95	1.0380099E-6	12.001161	6
AAGACGG	190	0.0	11.997806	5
CCCTAGA	80	2.8800867E-5	11.869035	1
>>END_MODULE