FastQCFastQC Report
Thu 2 Feb 2017
SRR4062617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252903
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24810.19802011807777617No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23760.18963958103699968No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12760.10184347870505538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGT852.3530083E-723.29390742
CGCAATA3750.020.53314636
ATACGAA4050.019.55537840
TACGAAT4250.019.15276741
TACTCCG708.124046E-418.8569725
CGGTCCA4300.018.41843810
CTTACGC600.007412638518.3331663
CGCCGGT4350.018.206737
AATACGA4400.017.99983839
TAGCGGC4300.017.90681530
CTAGCGG4300.017.90681529
TCTAGCG4350.017.70098928
CGAATGC4350.017.70098943
GGCGTAC750.001290984617.5998413
AGGCGTA750.001290984617.5998412
CAATACG4450.017.30321338
GCCGGTC4750.017.1366868
GGACCGT902.2156055E-417.1109566
CCGGTCC5200.016.9229249
CATCGTT4600.016.7389828
Produced by FastQC (version 0.11.5)