##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1252903
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24218714457544	32.0	32.0	32.0	32.0	32.0
2	31.416757721866738	32.0	32.0	32.0	32.0	32.0
3	31.500629338424442	32.0	32.0	32.0	32.0	32.0
4	31.606570500669246	32.0	32.0	32.0	32.0	32.0
5	31.55829302028968	32.0	32.0	32.0	32.0	32.0
6	35.13961735266018	36.0	36.0	36.0	36.0	36.0
7	35.152280743201985	36.0	36.0	36.0	36.0	36.0
8	35.09836276232079	36.0	36.0	36.0	36.0	36.0
9	35.22690743018414	36.0	36.0	36.0	36.0	36.0
10	35.10272383416753	36.0	36.0	36.0	36.0	36.0
11	35.233456221271716	36.0	36.0	36.0	36.0	36.0
12	35.14468877478943	36.0	36.0	36.0	36.0	36.0
13	35.184135563567175	36.0	36.0	36.0	36.0	36.0
14	35.13728836150923	36.0	36.0	36.0	36.0	36.0
15	35.09803951303493	36.0	36.0	36.0	36.0	36.0
16	35.1084042419884	36.0	36.0	36.0	36.0	36.0
17	35.09191134509216	36.0	36.0	36.0	36.0	36.0
18	35.096557355198286	36.0	36.0	36.0	36.0	36.0
19	35.082244196079024	36.0	36.0	36.0	36.0	36.0
20	35.07409033261154	36.0	36.0	36.0	36.0	36.0
21	35.05130085888533	36.0	36.0	36.0	36.0	36.0
22	35.035492771587265	36.0	36.0	36.0	36.0	36.0
23	34.985965393969046	36.0	36.0	36.0	36.0	36.0
24	34.96219100760394	36.0	36.0	36.0	36.0	36.0
25	34.93085657868167	36.0	36.0	36.0	32.0	36.0
26	34.89179928533973	36.0	36.0	36.0	32.0	36.0
27	34.84961006558369	36.0	36.0	36.0	32.0	36.0
28	34.83943210288426	36.0	36.0	36.0	32.0	36.0
29	34.80768822486657	36.0	36.0	36.0	32.0	36.0
30	34.78981692916371	36.0	36.0	36.0	32.0	36.0
31	34.77602416148736	36.0	36.0	36.0	32.0	36.0
32	34.734273922242984	36.0	36.0	36.0	32.0	36.0
33	34.703502984668404	36.0	36.0	36.0	32.0	36.0
34	34.685724273946185	36.0	36.0	36.0	32.0	36.0
35	34.62989393432692	36.0	36.0	36.0	32.0	36.0
36	34.60971439927911	36.0	36.0	36.0	32.0	36.0
37	34.58392868402422	36.0	36.0	36.0	32.0	36.0
38	34.53679494741412	36.0	36.0	36.0	32.0	36.0
39	34.47190165559505	36.0	36.0	36.0	32.0	36.0
40	34.42689418095415	36.0	36.0	36.0	32.0	36.0
41	34.419921574136225	36.0	36.0	36.0	32.0	36.0
42	34.331146146190086	36.0	36.0	36.0	32.0	36.0
43	34.34455101472341	36.0	36.0	36.0	32.0	36.0
44	34.32372258666473	36.0	36.0	36.0	32.0	36.0
45	34.2280463850753	36.0	36.0	36.0	32.0	36.0
46	34.275354117597296	36.0	36.0	36.0	32.0	36.0
47	34.213795481374056	36.0	36.0	36.0	32.0	36.0
48	34.19521223909592	36.0	36.0	36.0	32.0	36.0
49	34.205584151366864	36.0	36.0	36.0	32.0	36.0
50	33.671145332080776	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	13.0
21	39.0
22	129.0
23	406.0
24	1038.0
25	2455.0
26	5139.0
27	9504.0
28	15403.0
29	22284.0
30	30922.0
31	42852.0
32	61837.0
33	94270.0
34	204206.0
35	762403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81548537070565	17.403799178090065	11.425314442815951	26.355401008388334
2	16.106026719793366	20.288796619558227	36.321710271109005	27.283466389539402
3	18.76695075561061	24.068356940012485	28.449014840840487	28.71567746353642
4	12.461469452519031	15.576557464191135	35.376669524033005	36.58530355925683
5	14.162149823250484	36.758631753615404	33.63436754481393	15.44485087832019
6	35.00474501813004	34.65252020723238	16.681312769725746	13.661422004911833
7	30.18911295536283	30.367738258858235	20.916719743443622	18.526429042335316
8	28.655473452831902	32.6011930701683	19.584372919829324	19.158960557170474
9	27.38089061962498	14.105880503119556	18.284815344843135	40.228413532412326
10	15.612062545943301	26.968887455772713	32.0833296751624	25.33572032312158
11	37.77762524313534	20.92213044425626	21.53494723853323	19.765297074075168
12	24.081672723267484	23.736234967910523	28.94916845118896	23.232923857633033
13	30.222690822833055	19.191988525847574	24.94798080936832	25.63733984195105
14	23.656500144065422	19.19550036994085	25.02915229670613	32.1188471892876
15	25.654899062417442	26.566621677815444	22.85995005199924	24.918529207767882
16	26.09747123280892	25.421680688768404	23.991083108588615	24.489764969834056
17	24.16268458132832	25.5991884447559	25.70023377707612	24.53789319683966
18	25.419445878890862	24.14863720495521	26.625684510293297	23.80623240586063
19	25.25646438710738	25.795452640787037	25.258300123792505	23.68978284831308
20	25.362777485567516	23.81357535260112	26.133627264041987	24.690019897789377
21	26.272424920364944	24.514906580956385	24.28767430519362	24.924994193485052
22	25.176011231515925	24.70015635687679	24.893227967368585	25.2306044442387
23	23.999304016352426	24.269077494426945	25.414577185943365	26.317041303277268
24	24.40077164792486	25.458155978555403	25.14272852726827	24.998343846251466
25	25.227012785506936	24.15653885416509	24.99195867517278	25.624489685155194
26	24.334764941898936	24.900092026278173	25.60214158637979	25.1630014454431
27	25.63430688568868	24.93816360883484	24.863457107214206	24.564072398262276
28	24.5916882631776	24.149754609893982	25.74923996510504	25.50931716182338
29	24.24433495649703	25.07680163588083	26.036891922199885	24.641971485422257
30	24.975836118199094	24.595200107270873	26.028910458351522	24.400053316178507
31	24.63893852915988	24.78659561035451	25.0254010086974	25.549064851788206
32	24.53238598678429	24.932576584140993	25.034898950676947	25.500138478397773
33	24.227733511692445	24.44020007933575	25.548585963957304	25.7834804450145
34	24.58570216529133	24.89538296260764	25.964899118287686	24.554015753813342
35	25.72298094904394	24.456163007032465	25.555928910697794	24.264927133225797
36	24.631276323865457	25.2675586218566	25.21280577985686	24.888359274421084
37	25.67828475149313	24.8187609096634	25.270990651311394	24.231963687532076
38	24.55377630989789	24.837756793622493	25.241379420433987	25.36708747604563
39	24.857630638604906	24.644126480661313	25.29501485749495	25.203228023238832
40	25.376505603386697	24.767519911756935	25.200913398722808	24.655061086133564
41	23.98541626925628	25.047828922111286	25.878858938002384	25.08789587063005
42	25.884286333419265	25.151508137501466	25.199237291314653	23.764968237764617
43	25.013189369009414	23.88987814699143	25.310419082722284	25.786513401276874
44	24.534062094192446	24.740622378587968	25.74772348697385	24.97759204024573
45	24.813253699608033	24.734716095340183	25.417051439736355	25.03497876531543
46	24.318482755648283	24.488408120979837	25.925390872238314	25.26771825113357
47	24.766641950733618	24.34242714719336	26.210249316986236	24.680681585086795
48	24.98948442137979	25.85587232211911	24.679165106955605	24.475478149545495
49	24.044558916372615	25.120220799215904	25.86505100554472	24.97016927886676
50	24.020793326213862	26.077857645689768	24.674316107724305	25.227032920372068
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	26.0
2	29.0
3	144.0
4	259.0
5	258.5
6	258.0
7	253.0
8	248.0
9	336.0
10	424.0
11	652.5
12	881.0
13	1498.5
14	2116.0
15	2743.5
16	3371.0
17	3828.5
18	4286.0
19	4572.0
20	4858.0
21	5580.5
22	6303.0
23	6724.0
24	7145.0
25	7701.5
26	8258.0
27	9910.0
28	11562.0
29	13271.5
30	14981.0
31	16532.5
32	18084.0
33	20795.5
34	23507.0
35	27189.5
36	30872.0
37	36490.0
38	42108.0
39	46201.0
40	50294.0
41	56027.5
42	61761.0
43	65183.0
44	68605.0
45	78037.0
46	87469.0
47	95223.5
48	102978.0
49	102919.5
50	102861.0
51	97239.5
52	91618.0
53	89408.0
54	87198.0
55	87378.5
56	87559.0
57	84790.0
58	82021.0
59	75669.5
60	69318.0
61	61132.5
62	52947.0
63	45893.0
64	38839.0
65	33282.0
66	27725.0
67	23796.0
68	19867.0
69	18058.5
70	16250.0
71	12625.5
72	9001.0
73	8066.5
74	7132.0
75	5103.5
76	3075.0
77	2802.5
78	2530.0
79	2143.0
80	1756.0
81	1407.0
82	1058.0
83	889.0
84	720.0
85	585.5
86	451.0
87	332.5
88	214.0
89	133.5
90	53.0
91	33.0
92	13.0
93	9.5
94	6.0
95	5.0
96	4.0
97	4.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016840888720036588
2	0.0039109172856957
3	7.183317463522714E-4
4	7.981463848358572E-5
5	0.0
6	7.981463848358573E-4
7	7.981463848358572E-5
8	7.981463848358572E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	7.981463848358572E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1252903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.09133674119189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74877788746392	47.715532255878394
2	12.123887063132187	14.328333861194482
3	3.2191619110062457	5.706737415230665
4	1.3472005307729915	3.1843152088727713
5	0.6749185748503662	1.9940920389684154
6	0.4097042432516045	1.4525982841365452
7	0.2887364458167399	1.194327578444832
8	0.17792203849534252	0.8410920872326071
9	0.14685326557157402	0.7809980190690206
>10	0.7162257795605458	7.670656787410375
>50	0.06846035846710184	2.8499072488320643
>100	0.06946837094533255	8.273209232742513
>500	0.007326728999553998	2.8915194597317986
>1k	0.0013568016665840739	1.1166805222554965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2481	0.19802011807777617	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2376	0.18963958103699968	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1276	0.10184347870505538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.981463848358572E-5
2	0.0	0.0	0.0	0.0	7.981463848358572E-5
3	0.0	0.0	0.0	0.0	7.981463848358572E-5
4	0.0	0.0	0.0	0.0	7.981463848358572E-5
5	0.0	0.0	0.0	0.0	7.981463848358572E-5
6	0.0	0.0	0.0	0.0	1.5962927696717145E-4
7	0.0	0.0	0.0	0.0	1.5962927696717145E-4
8	0.0	0.0	0.0	0.0	1.5962927696717145E-4
9	0.0	0.0	0.0	7.981463848358572E-5	1.5962927696717145E-4
10	0.0	0.0	0.0	7.981463848358572E-5	1.5962927696717145E-4
11	0.0	0.0	0.0	7.981463848358572E-5	1.5962927696717145E-4
12	0.0	0.0	0.0	1.5962927696717145E-4	3.990731924179286E-4
13	0.0	0.0	0.0	1.5962927696717145E-4	4.788878309015143E-4
14	0.0	0.0	0.0	3.192585539343429E-4	4.788878309015143E-4
15	0.0	0.0	0.0	3.192585539343429E-4	6.385171078686858E-4
16	0.0	0.0	0.0	3.192585539343429E-4	6.385171078686858E-4
17	0.0	0.0	0.0	4.788878309015143E-4	6.385171078686858E-4
18	0.0	0.0	0.0	5.587024693851E-4	6.385171078686858E-4
19	0.0	0.0	0.0	6.385171078686858E-4	6.385171078686858E-4
20	0.0	0.0	0.0	7.981463848358572E-4	7.183317463522714E-4
21	0.0	0.0	0.0	0.0010375903002866143	7.183317463522714E-4
22	0.0	0.0	0.0	0.0016761074081553002	7.183317463522714E-4
23	0.0	0.0	0.0	0.0027935123469255	7.183317463522714E-4
24	0.0	0.0	0.0	0.004868692947498729	7.981463848358572E-4
25	0.0	0.0	0.0	0.0059062832477853435	7.981463848358572E-4
26	0.0	0.0	0.0	0.009418127341063115	8.77961023319443E-4
27	0.0	0.0	0.0	0.012371268964955787	8.77961023319443E-4
28	0.0	0.0	0.0	0.019873844982412844	8.77961023319443E-4
29	0.0	0.0	0.0	0.03160659683949994	8.77961023319443E-4
30	0.0	0.0	0.0	0.05259784676068299	8.77961023319443E-4
31	0.0	0.0	0.0	0.09489960515698342	8.77961023319443E-4
32	0.0	0.0	0.0	0.1448635688477081	8.77961023319443E-4
33	0.0	0.0	0.0	0.19977604012441505	8.77961023319443E-4
34	0.0	0.0	0.0	0.26330849235734927	8.77961023319443E-4
35	0.0	0.0	0.0	0.33186926681474943	8.77961023319443E-4
36	0.0	0.0	0.0	0.43427144798918993	8.77961023319443E-4
37	0.0	0.0	0.0	0.5848816708077161	8.77961023319443E-4
38	0.0	0.0	0.0	0.7683755246814797	8.77961023319443E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTCGT	85	2.3530083E-7	23.293907	42
CGCAATA	375	0.0	20.533146	36
ATACGAA	405	0.0	19.555378	40
TACGAAT	425	0.0	19.152767	41
TACTCCG	70	8.124046E-4	18.856972	5
CGGTCCA	430	0.0	18.418438	10
CTTACGC	60	0.0074126385	18.333166	3
CGCCGGT	435	0.0	18.20673	7
AATACGA	440	0.0	17.999838	39
TAGCGGC	430	0.0	17.906815	30
CTAGCGG	430	0.0	17.906815	29
TCTAGCG	435	0.0	17.700989	28
CGAATGC	435	0.0	17.700989	43
GGCGTAC	75	0.0012909846	17.59984	13
AGGCGTA	75	0.0012909846	17.59984	12
CAATACG	445	0.0	17.303213	38
GCCGGTC	475	0.0	17.136686	8
GGACCGT	90	2.2156055E-4	17.110956	6
CCGGTCC	520	0.0	16.922924	9
CATCGTT	460	0.0	16.73898	28
>>END_MODULE