FastQCFastQC Report
Thu 2 Feb 2017
SRR4062616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368577
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG133943.6339760755554473No Hit
TCCATGTACTCTGCGTTGATACCAC114283.1005732859076938No Hit
GAGTACATGGAAGCAGTGGTATCAA59591.6167585063636636No Hit
CATGTACTCTGCGTTGATACCACTG51791.4051337983650636No Hit
GCTTCCATGTACTCTGCGTTGATAC36050.9780859901730168No Hit
CCCATGTACTCTGCGTTGATACCAC31800.8627776556866001No Hit
GCGTTGATACCACTGCTTCCATGTA30530.8283208122047768No Hit
CATGGAAGCAGTGGTATCAACGCAG30500.8275068710201668No Hit
GTACATGGGAAGCAGTGGTATCAAC28100.7623915762513667No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22950.62266500622665No Hit
GTATCAACGCAGAGTACATGGAAGC22830.6194092414882101No Hit
GTATCAACGCAGAGTACTTTTTTTT22220.6028591040678067No Hit
ACTCTGCGTTGATACCACTGCTTCC19430.5271625738990767No Hit
ACGCAGAGTACATGGAAGCAGTGGT18950.5141395149453167No Hit
CAGTGGTATCAACGCAGAGTACATG18480.5013877697197601No Hit
GTCCTACAGTGGACATTTCTAAATT15480.41999365125876004No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15410.4180944551613367No Hit
TATCAACGCAGAGTACATGGAAGCA15030.4077845334896101No Hit
TATCAACGCAGAGTACTTTTTTTTT15020.4075132197614067No Hit
GTCCTAAAGTGTGTATTTCTCATTT13990.3795679057564634No Hit
CTGTAGGACGTGGAATATGGCAAGA12240.33208800332088No Hit
CTTTAGGACGTGAAATATGGCGAGG12190.3307314346798634No Hit
GTACATGGGGTGGTATCAACGCAAA11200.30387137558773336No Hit
GGTATCAACGCAGAGTACATGGAAG11000.29844510102366667No Hit
GTATCAACGCAGAGTACATGGGAAG10850.2943753951006167No Hit
ATACCACTGCTTCCATGTACTCTGC10700.2903056891775667No Hit
GTCCTACAGTGTGCATTTCTCATTT10640.2886778068083467No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10380.28162364987506006No Hit
AAGCAGTGGTATCAACGCAGAGTAC10150.2753834341263834No Hit
GAGTACATGGGAAGCAGTGGTATCA9810.26615876736747No Hit
CTGTAGGACCTGGAATATGGCGAGA9040.24526761029581337No Hit
TTGTAGAACAGTGTATATCAATGAG8830.23957002200354335No Hit
GCGTTGATACCACTGCTTCCCATGT8760.23767082590612004No Hit
CCACTGCTTCCATGTACTCTGCGTT8560.23224455134205338No Hit
GTGTATATCAATGAGTTACAATGAA8540.23170192388564667No Hit
GTACTCTGCGTTGATACCACTGCTT8200.22247725712673336No Hit
GTGGTATCAACGCAGAGTACATGGA8030.2178649237472767No Hit
GCTTCCCATGTACTCTGCGTTGATA7880.21379521782422667No Hit
GGTATCAACGCAGAGTACTTTTTTT7870.21352390409602337No Hit
AAAAAGTACTCTGCGTTGATACCAC7720.20945419817297337No Hit
ACATGGAAGCAGTGGTATCAACGCA7090.19236143329616334No Hit
TATCAACGCAGAGTACATGGGAAGC6970.18910566855772334No Hit
GGTATCAACGCAGAGTACATGGGAA6840.18557859009108002No Hit
GTACATGGGTGGTATCAACGCAAAA6790.18422202145006336No Hit
GATATACACTGTTCTACAAATCCCG6780.18395070772186No Hit
CTGCTTCCATGTACTCTGCGTTGAT6650.18042362925521668No Hit
GAAGCAGTGGTATCAACGCAGAGTA6400.17364078605013336No Hit
GTTGATACCACTGCTTCCATGTACT6350.17228421740911667No Hit
GATATACACTGTTCTACAATGCCGG6310.17119896249630334No Hit
TGGAAGCAGTGGTATCAACGCAGAG6130.16631531538864333No Hit
CTGAAGGACCTGGAATATGGCGAGA6110.16577268793223668No Hit
CCCCATGTACTCTGCGTTGATACCA5810.1576332760861367No Hit
ACGCAGAGTACATGGGAAGCAGTGG5780.1568193349015267No Hit
GTTCTACAGTGTGGTTTTTATCATT5670.15383488389129002No Hit
GATACCACTGCTTCCATGTACTCTG5650.15329225643488334No Hit
ATTTAGAAATGTCCACTGTAGGACG5440.14759466814261335No Hit
GTACATGGGAGTGGTATCAACGCAA5430.14732335441441002No Hit
GTGTATATCAATGAGTTACAATGAG5420.14705204068620667No Hit
GTCCTTCAGTGTGCATTTCTCATTT5310.14406758967597No Hit
GCAGAGTACATGGAAGCAGTGGTAT5090.13809868765549668No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4820.13077321699400668No Hit
TTTCTAAATTTTCCACCTTTTTCAG4680.12697482479916No Hit
CTTCCATGTACTCTGCGTTGATACC4490.12181986396329668No Hit
CCATGTACTCTGCGTTGATACCACT4410.11964935413767No Hit
GCAGTGGTATCAACGCAGAGTACAT4310.11693621685563667No Hit
GTATTAGAGGCACTGCCTGCCCAGT4260.11557964821462001No Hit
CTGTAGAACATATTAGATGAGTGAG4180.11340913838899333No Hit
CCATAGGGTCTTCTCGTCTTATTAT4010.10879680500953667No Hit
ATGTTCTACAGTGTGGTTTTTATCA3920.10635498145570668No Hit
GTATCAACGCAGAGTACATGGGGTG3750.10174264807625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGGT307.696303E-419.0040811
GTCGATA250.00601605919.0040789
GGGTCAA250.00601605919.00407811
TTGTCGA250.00601605919.0040787
ATTGTCG250.00601605919.0040786
TGTCGAT250.00601605919.0040788
CCAATTT451.6601025E-619.00149514
CCCAATT451.6601025E-619.00149513
CTAATTG307.7315664E-418.991173
CGGTATC307.75279E-418.9834352
TCCGGTG402.7465777E-416.63082912
CCCAATG402.7723296E-416.6105042
CGACAAT350.002162863516.2892117
TATGTCG350.002162863516.2892116
GGTATCC350.002172706216.2781453
GTATTAA350.002180607516.2693041
TCCCAAT350.002180607516.2693041
CCAACGA551.1199161E-515.55090619
CACCTTT1100.015.54667814
TCCAACG1055.456968E-1215.38425518
Produced by FastQC (version 0.11.5)