##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062616_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368577
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.921172509407803	32.0	32.0	32.0	32.0	32.0
2	30.910832743225974	32.0	32.0	32.0	32.0	32.0
3	30.92164459529488	32.0	32.0	32.0	32.0	32.0
4	30.849214682413717	32.0	32.0	32.0	32.0	32.0
5	30.89262759206353	32.0	32.0	32.0	32.0	32.0
6	34.44331035306055	36.0	36.0	36.0	32.0	36.0
7	34.21813895061276	36.0	36.0	36.0	32.0	36.0
8	34.21878739042317	36.0	36.0	36.0	32.0	36.0
9	34.21871684885384	36.0	36.0	36.0	32.0	36.0
10	34.15865341570418	36.0	36.0	36.0	32.0	36.0
11	34.38502131169335	36.0	36.0	36.0	32.0	36.0
12	34.23248873369744	36.0	36.0	36.0	32.0	36.0
13	34.246667589133345	36.0	36.0	36.0	32.0	36.0
14	34.194751707241636	36.0	36.0	36.0	32.0	36.0
15	34.25687169845107	36.0	36.0	36.0	32.0	36.0
16	34.193639320956	36.0	36.0	36.0	32.0	36.0
17	34.134745249974905	36.0	36.0	36.0	32.0	36.0
18	34.171551670342964	36.0	36.0	36.0	32.0	36.0
19	34.096145445863414	36.0	36.0	36.0	32.0	36.0
20	34.04008660334204	36.0	36.0	36.0	32.0	36.0
21	33.86455476060633	36.0	36.0	36.0	32.0	36.0
22	33.83636255110872	36.0	36.0	36.0	32.0	36.0
23	33.7056218917621	36.0	36.0	36.0	27.0	36.0
24	33.712608220263334	36.0	36.0	36.0	27.0	36.0
25	33.05666929840983	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	14.0
5	36.0
6	60.0
7	15.0
8	54.0
9	83.0
10	32.0
11	13.0
12	24.0
13	19.0
14	111.0
15	155.0
16	207.0
17	301.0
18	403.0
19	593.0
20	810.0
21	1069.0
22	1551.0
23	1957.0
24	2481.0
25	3221.0
26	4146.0
27	5160.0
28	6717.0
29	9191.0
30	11981.0
31	16642.0
32	24940.0
33	36230.0
34	79960.0
35	160400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.03867578706882	17.694159017340883	13.207670037525334	23.059495158064962
2	9.958379006088494	23.03317741287809	42.994975092521244	24.01346848851217
3	20.708601383232445	29.28393708435873	28.677246667408298	21.330214865000528
4	11.003020213450124	18.76819793930809	36.94836330481362	33.280418542428166
5	9.651863090213789	41.41002640814011	36.548540224238494	12.389570277407605
6	29.55539328923188	35.45561397746428	20.956152804206376	14.03283992909746
7	24.47302404378706	33.271703481679374	23.740796246823486	18.514476227710084
8	26.120724302364735	37.22640423197662	22.847079653707866	13.80579181195077
9	26.618615160666565	16.998989723423207	19.320181632520043	37.06221348339018
10	14.71450263074261	30.05837342148653	35.734638609033894	19.492485338736973
11	33.45022504597348	22.556478797871502	23.07556492379403	20.917731232360985
12	21.598030985892535	25.863413229449367	33.72126823198941	18.81728755266868
13	33.33025486963159	22.67560145484573	25.336208980602965	18.65793469491972
14	22.7196049189546	22.565038695884844	26.328685480825907	28.386670904334643
15	24.568198670711933	30.264798311635754	29.147370622570733	16.01963239508158
16	22.599999456783017	26.305010905080977	33.07132167408611	18.0236679640499
17	20.11843320475906	27.911120769272557	33.222415385451185	18.748030640517193
18	22.47840260798696	23.56234718826406	37.701168160825866	16.25808204292312
19	21.445880013474305	32.72653677725015	26.469949036695752	19.357634172579786
20	20.392956874164703	25.521552051981356	37.00303152130214	17.0824595525518
21	20.335175194101698	29.604787751354227	24.298164115771	25.761872938773074
22	18.983534398434955	33.190142375828714	26.891370503206176	20.93495272253016
23	18.250729496660888	31.368899804924173	26.94219995544229	23.438170742972652
24	19.452798356928042	33.19939906164508	28.13597724475741	19.211825336669463
25	23.513846856000303	28.33240061070693	26.57362984857293	21.58012268471983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	138.0
2	353.5
3	569.0
4	569.0
5	569.0
6	1820.5
7	3072.0
8	3072.0
9	3072.0
10	2817.5
11	2563.0
12	2563.0
13	2563.0
14	2081.5
15	1600.0
16	1600.0
17	1600.0
18	2679.5
19	3759.0
20	3759.0
21	3759.0
22	6574.0
23	9389.0
24	9389.0
25	9389.0
26	14760.0
27	20131.0
28	20131.0
29	20131.0
30	27183.5
31	34236.0
32	34236.0
33	34236.0
34	34490.5
35	34745.0
36	34745.0
37	34745.0
38	34356.0
39	33967.0
40	33967.0
41	33967.0
42	39267.5
43	44568.0
44	44568.0
45	44568.0
46	70801.0
47	97034.0
48	97034.0
49	97034.0
50	70898.5
51	44763.0
52	44763.0
53	44763.0
54	30259.5
55	15756.0
56	15756.0
57	15756.0
58	12296.5
59	8837.0
60	8837.0
61	8837.0
62	7141.0
63	5445.0
64	5445.0
65	5445.0
66	4505.5
67	3566.0
68	3566.0
69	3566.0
70	2851.0
71	2136.0
72	2136.0
73	2136.0
74	1699.0
75	1262.0
76	1262.0
77	1262.0
78	908.0
79	554.0
80	554.0
81	554.0
82	376.0
83	198.0
84	198.0
85	198.0
86	129.5
87	61.0
88	61.0
89	61.0
90	44.5
91	28.0
92	28.0
93	28.0
94	22.0
95	16.0
96	16.0
97	16.0
98	100.0
99	184.0
100	184.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007054156933286667
2	0.0035270784666433337
3	0.0054262745640666675
4	0.0162788236922
5	0.03527078466643334
6	0.049921725989413336
7	0.06755711832263
8	0.09034747149171
9	0.09794425588140335
10	0.11666490312743334
11	0.11666490312743334
12	0.12860270716838001
13	0.11612227567102669
14	0.1220911776915
15	0.11123862856336668
16	0.10852549128133335
17	0.11856409922485667
18	0.12941664835299002
19	0.12724613852736336
20	0.12073460905048335
21	0.12778876598377004
22	0.14569547204519
23	0.1397265700247167
24	0.13185847190682
25	0.12995927580939667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	368577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.139868923658845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34132017472105	19.7939552963377
2	15.43539646406122	8.6870006656678
3	5.353123773840313	4.519086039839651
4	2.520327009745537	2.836866867959858
5	1.3443764969293113	1.891528920381923
6	0.8769333892014344	1.4806074376144962
7	0.6148446966295849	1.2111154420094312
8	0.4404554615291159	0.9915487163311185
9	0.3069236596547703	0.7773112397029424
>10	2.2281859806414217	12.600181157490303
>50	0.27304246795310205	5.438935186260639
>100	0.20528010364357308	10.861606285039162
>500	0.030891666082285267	6.036219778276268
>1k	0.024912633937326827	12.790302728176957
>5k	0.001993010714986146	3.1232656271690553
>10k+	0.001993010714986146	6.960468611742708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	13394	3.6339760755554473	No Hit
TCCATGTACTCTGCGTTGATACCAC	11428	3.1005732859076938	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5959	1.6167585063636636	No Hit
CATGTACTCTGCGTTGATACCACTG	5179	1.4051337983650636	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3605	0.9780859901730168	No Hit
CCCATGTACTCTGCGTTGATACCAC	3180	0.8627776556866001	No Hit
GCGTTGATACCACTGCTTCCATGTA	3053	0.8283208122047768	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3050	0.8275068710201668	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2810	0.7623915762513667	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2295	0.62266500622665	No Hit
GTATCAACGCAGAGTACATGGAAGC	2283	0.6194092414882101	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2222	0.6028591040678067	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1943	0.5271625738990767	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1895	0.5141395149453167	No Hit
CAGTGGTATCAACGCAGAGTACATG	1848	0.5013877697197601	No Hit
GTCCTACAGTGGACATTTCTAAATT	1548	0.41999365125876004	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1541	0.4180944551613367	No Hit
TATCAACGCAGAGTACATGGAAGCA	1503	0.4077845334896101	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1502	0.4075132197614067	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1399	0.3795679057564634	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1224	0.33208800332088	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1219	0.3307314346798634	No Hit
GTACATGGGGTGGTATCAACGCAAA	1120	0.30387137558773336	No Hit
GGTATCAACGCAGAGTACATGGAAG	1100	0.29844510102366667	No Hit
GTATCAACGCAGAGTACATGGGAAG	1085	0.2943753951006167	No Hit
ATACCACTGCTTCCATGTACTCTGC	1070	0.2903056891775667	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1064	0.2886778068083467	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1038	0.28162364987506006	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1015	0.2753834341263834	No Hit
GAGTACATGGGAAGCAGTGGTATCA	981	0.26615876736747	No Hit
CTGTAGGACCTGGAATATGGCGAGA	904	0.24526761029581337	No Hit
TTGTAGAACAGTGTATATCAATGAG	883	0.23957002200354335	No Hit
GCGTTGATACCACTGCTTCCCATGT	876	0.23767082590612004	No Hit
CCACTGCTTCCATGTACTCTGCGTT	856	0.23224455134205338	No Hit
GTGTATATCAATGAGTTACAATGAA	854	0.23170192388564667	No Hit
GTACTCTGCGTTGATACCACTGCTT	820	0.22247725712673336	No Hit
GTGGTATCAACGCAGAGTACATGGA	803	0.2178649237472767	No Hit
GCTTCCCATGTACTCTGCGTTGATA	788	0.21379521782422667	No Hit
GGTATCAACGCAGAGTACTTTTTTT	787	0.21352390409602337	No Hit
AAAAAGTACTCTGCGTTGATACCAC	772	0.20945419817297337	No Hit
ACATGGAAGCAGTGGTATCAACGCA	709	0.19236143329616334	No Hit
TATCAACGCAGAGTACATGGGAAGC	697	0.18910566855772334	No Hit
GGTATCAACGCAGAGTACATGGGAA	684	0.18557859009108002	No Hit
GTACATGGGTGGTATCAACGCAAAA	679	0.18422202145006336	No Hit
GATATACACTGTTCTACAAATCCCG	678	0.18395070772186	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	665	0.18042362925521668	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	640	0.17364078605013336	No Hit
GTTGATACCACTGCTTCCATGTACT	635	0.17228421740911667	No Hit
GATATACACTGTTCTACAATGCCGG	631	0.17119896249630334	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	613	0.16631531538864333	No Hit
CTGAAGGACCTGGAATATGGCGAGA	611	0.16577268793223668	No Hit
CCCCATGTACTCTGCGTTGATACCA	581	0.1576332760861367	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	578	0.1568193349015267	No Hit
GTTCTACAGTGTGGTTTTTATCATT	567	0.15383488389129002	No Hit
GATACCACTGCTTCCATGTACTCTG	565	0.15329225643488334	No Hit
ATTTAGAAATGTCCACTGTAGGACG	544	0.14759466814261335	No Hit
GTACATGGGAGTGGTATCAACGCAA	543	0.14732335441441002	No Hit
GTGTATATCAATGAGTTACAATGAG	542	0.14705204068620667	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	531	0.14406758967597	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	509	0.13809868765549668	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	482	0.13077321699400668	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	468	0.12697482479916	No Hit
CTTCCATGTACTCTGCGTTGATACC	449	0.12181986396329668	No Hit
CCATGTACTCTGCGTTGATACCACT	441	0.11964935413767	No Hit
GCAGTGGTATCAACGCAGAGTACAT	431	0.11693621685563667	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	426	0.11557964821462001	No Hit
CTGTAGAACATATTAGATGAGTGAG	418	0.11340913838899333	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	401	0.10879680500953667	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	392	0.10635498145570668	No Hit
GTATCAACGCAGAGTACATGGGGTG	375	0.10174264807625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.713137282033334E-4	0.0
10	0.0	0.0	0.0	2.713137282033334E-4	0.0
11	0.0	0.0	0.0	2.713137282033334E-4	0.0
12	0.0	0.0	0.0	2.713137282033334E-4	0.0
13	0.0	0.0	0.0	2.713137282033334E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCGGT	30	7.696303E-4	19.00408	11
GTCGATA	25	0.006016059	19.004078	9
GGGTCAA	25	0.006016059	19.004078	11
TTGTCGA	25	0.006016059	19.004078	7
ATTGTCG	25	0.006016059	19.004078	6
TGTCGAT	25	0.006016059	19.004078	8
CCAATTT	45	1.6601025E-6	19.001495	14
CCCAATT	45	1.6601025E-6	19.001495	13
CTAATTG	30	7.7315664E-4	18.99117	3
CGGTATC	30	7.75279E-4	18.983435	2
TCCGGTG	40	2.7465777E-4	16.630829	12
CCCAATG	40	2.7723296E-4	16.610504	2
CGACAAT	35	0.0021628635	16.28921	17
TATGTCG	35	0.0021628635	16.28921	16
GGTATCC	35	0.0021727062	16.278145	3
GTATTAA	35	0.0021806075	16.269304	1
TCCCAAT	35	0.0021806075	16.269304	1
CCAACGA	55	1.1199161E-5	15.550906	19
CACCTTT	110	0.0	15.546678	14
TCCAACG	105	5.456968E-12	15.384255	18
>>END_MODULE