##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062616_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368577
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98277157825909	32.0	32.0	32.0	32.0	32.0
2	31.468952213513052	32.0	32.0	32.0	32.0	32.0
3	31.542909622683997	32.0	32.0	32.0	32.0	32.0
4	31.661902397599416	32.0	32.0	32.0	32.0	32.0
5	31.62489520507248	32.0	32.0	32.0	32.0	32.0
6	35.22517140244779	36.0	36.0	36.0	36.0	36.0
7	35.227906244828084	36.0	36.0	36.0	36.0	36.0
8	35.19605943941158	36.0	36.0	36.0	36.0	36.0
9	35.2425816043866	36.0	36.0	36.0	36.0	36.0
10	35.19127346524607	36.0	36.0	36.0	36.0	36.0
11	35.25918329141536	36.0	36.0	36.0	36.0	36.0
12	35.18503596263467	36.0	36.0	36.0	36.0	36.0
13	35.20849374757513	36.0	36.0	36.0	36.0	36.0
14	35.169972624444824	36.0	36.0	36.0	36.0	36.0
15	35.15277133407673	36.0	36.0	36.0	36.0	36.0
16	35.120856700228174	36.0	36.0	36.0	36.0	36.0
17	35.128095350496636	36.0	36.0	36.0	36.0	36.0
18	35.11801061921932	36.0	36.0	36.0	36.0	36.0
19	35.03069100893436	36.0	36.0	36.0	36.0	36.0
20	35.00760221066426	36.0	36.0	36.0	36.0	36.0
21	34.861784647441375	36.0	36.0	36.0	32.0	36.0
22	34.8170070297387	36.0	36.0	36.0	32.0	36.0
23	34.697243723835186	36.0	36.0	36.0	32.0	36.0
24	34.69995143484265	36.0	36.0	36.0	32.0	36.0
25	34.64322244741262	36.0	36.0	36.0	32.0	36.0
26	34.55905550264938	36.0	36.0	36.0	32.0	36.0
27	34.45944538047681	36.0	36.0	36.0	32.0	36.0
28	34.35091717605819	36.0	36.0	36.0	32.0	36.0
29	34.27041839290026	36.0	36.0	36.0	32.0	36.0
30	34.19009596366566	36.0	36.0	36.0	32.0	36.0
31	34.13237939426497	36.0	36.0	36.0	32.0	36.0
32	34.04965312539849	36.0	36.0	36.0	32.0	36.0
33	33.98619013123445	36.0	36.0	36.0	32.0	36.0
34	33.89302642324399	36.0	36.0	36.0	32.0	36.0
35	33.69145117573804	36.0	36.0	36.0	27.0	36.0
36	33.59347436220926	36.0	36.0	36.0	21.0	36.0
37	33.33977432124088	36.0	36.0	36.0	21.0	36.0
38	33.18370923850376	36.0	36.0	36.0	14.0	36.0
39	32.693618972426385	36.0	36.0	36.0	14.0	36.0
40	32.471947517072415	36.0	36.0	36.0	14.0	36.0
41	32.565377112516515	36.0	36.0	36.0	14.0	36.0
42	32.09075986835858	36.0	36.0	36.0	14.0	36.0
43	32.24086418848707	36.0	36.0	36.0	14.0	36.0
44	32.238126632969504	36.0	36.0	36.0	14.0	36.0
45	31.69366509576018	36.0	36.0	36.0	14.0	36.0
46	32.01430365975088	36.0	36.0	36.0	14.0	36.0
47	31.745456173336915	36.0	36.0	36.0	14.0	36.0
48	31.805017676089392	36.0	36.0	36.0	14.0	36.0
49	31.96021184175898	36.0	36.0	36.0	14.0	36.0
50	31.210458058967326	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	10.0
22	47.0
23	174.0
24	579.0
25	1468.0
26	3185.0
27	6310.0
28	10427.0
29	14181.0
30	17804.0
31	23085.0
32	32180.0
33	32734.0
34	54215.0
35	172173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.690452801311245	18.421409692108135	13.626372433556034	23.26176507302459
2	9.789509669857393	23.64204163139502	42.46190662238718	24.1065420763604
3	20.696793588261787	29.27661744995578	28.805884300032012	21.22070466175042
4	10.971927168542802	18.90025693410061	37.099167880795605	33.02864801656099
5	9.591211605716039	41.432048120202836	36.52805248292758	12.448687791153544
6	29.87199383573537	35.754362736586216	20.332577270359437	14.04106615731898
7	24.101069790030305	33.208800332088	23.957273514082537	18.73285636379915
8	26.154643398801337	37.301025294578885	22.832406796951517	13.711924509668263
9	26.46990995097361	16.491262341383212	19.62086619620866	37.41796151143452
10	14.800435187220037	30.07105706541645	35.751009965353234	19.377497782010273
11	33.64100310111591	22.23036163406832	23.33053880193284	20.798096462882924
12	21.42455986130442	25.952785985018057	33.946502359072866	18.676151794604657
13	33.66243688564398	22.383653890503204	25.574574647902608	18.37933457595021
14	22.76729150218272	22.23822973218622	26.6234735211367	28.37100524449437
15	24.68819269786232	30.355665166301748	29.111149095033063	15.844993040802871
16	22.68264161898328	26.075962417622367	33.0707016444325	18.170694318961843
17	20.091595514641444	27.796634081887913	33.53654731575763	18.575223087713017
18	22.714927952639474	23.001977877078602	38.07074234149174	16.212351828790187
19	21.743896119399743	32.13819636059765	26.954747583272965	19.163159936729638
20	20.565309284084464	25.239773507299695	37.2128483329128	16.982068875703042
21	20.400621851065043	29.093785016428047	24.740013619949156	25.765579512557757
22	19.13711381882212	32.40761089270356	27.501444745602683	20.95383054287164
23	18.274878790591924	30.50950005019304	27.409740705469954	23.80588045374508
24	19.677028137946753	32.47191224628775	28.51724334399596	19.333816271769535
25	24.152348084660737	27.14249668318967	26.92083336724755	21.784321864902044
26	15.7901876677058	28.254882968823342	31.663126022513616	24.291803340957248
27	22.14625437832529	30.898292622708418	30.43054775528587	16.524905243680426
28	19.874273218350574	26.49161504922988	28.723984404886906	24.910127327532646
29	18.105036396736637	29.696101493039446	31.385300764833403	20.813561345390514
30	23.48844339174718	26.20510775224716	30.758023425227293	19.548425430778373
31	18.870412423998243	27.352222195090846	30.10795573245211	23.669409648458803
32	18.149260534433783	30.84402987706775	30.83724703386267	20.169462554635803
33	17.874962355220212	29.637497727747526	31.32913882309531	21.158401093936952
34	16.48800657664477	32.17021138052564	34.134251458989574	17.20753058384001
35	21.077278289204155	30.240628145543536	30.630777286699928	18.051316278552378
36	18.90541189493647	30.900463132534046	31.44092007911508	18.753204893414402
37	21.17196678034712	29.932958377760954	29.708581924536798	19.186492917355125
38	20.517286754192476	31.82971265163046	25.63480629556375	22.018194298613317
39	19.916869473678496	28.601350599738996	30.89774999525201	20.584029931330495
40	24.09347300564061	28.882702935885852	27.12757442813849	19.896249630335046
41	19.004170092002486	27.588807766084155	32.159901458853916	21.247120683059443
42	23.51611739202392	31.08170070297387	29.328742705052136	16.07343919995008
43	20.35992479183454	28.464337166996312	28.417399892017137	22.758338149152006
44	19.335715467866958	29.031925486397686	33.225350469508406	18.40700857622695
45	21.389289076637986	29.573739001619742	29.19498503704789	19.84198688469438
46	19.52297490883834	27.060362042021186	32.34475386351797	21.071909185622502
47	23.79102274700469	26.00549140475777	31.77987714881056	18.423608699426982
48	18.189957593664282	34.966641977117405	25.764494257644944	21.078906171573376
49	18.753476207142604	27.22687525266091	34.951719722066215	19.06792881813027
50	17.272917192337015	35.78383892646584	23.945878337498	22.997365543699146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14.0
1	17.0
2	20.0
3	92.5
4	165.0
5	169.0
6	173.0
7	167.0
8	161.0
9	214.0
10	267.0
11	385.0
12	503.0
13	932.5
14	1362.0
15	1897.5
16	2433.0
17	3023.5
18	3614.0
19	3952.0
20	4290.0
21	4750.5
22	5211.0
23	5132.5
24	5054.0
25	5326.0
26	5598.0
27	7766.0
28	9934.0
29	11193.0
30	12452.0
31	13548.5
32	14645.0
33	18179.5
34	21714.0
35	24635.0
36	27556.0
37	27876.5
38	28197.0
39	25759.0
40	23321.0
41	22461.5
42	21602.0
43	21844.0
44	22086.0
45	28197.0
46	34308.0
47	39204.0
48	44100.0
49	37185.0
50	30270.0
51	22668.0
52	15066.0
53	11514.0
54	7962.0
55	7019.0
56	6076.0
57	5539.5
58	5003.0
59	4385.5
60	3768.0
61	3365.0
62	2962.0
63	2570.5
64	2179.0
65	2022.5
66	1866.0
67	1624.0
68	1382.0
69	1236.5
70	1091.0
71	950.0
72	809.0
73	668.0
74	527.0
75	462.0
76	397.0
77	298.0
78	199.0
79	159.0
80	119.0
81	86.0
82	53.0
83	41.0
84	29.0
85	24.5
86	20.0
87	13.0
88	6.0
89	5.5
90	5.0
91	6.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019263274702436668
2	0.0024418235538300006
3	8.1394118461E-4
4	0.0
5	0.0
6	2.713137282033334E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.713137282033334E-4
47	2.713137282033334E-4
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	368577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.53824012072104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7080726340182	35.38688870926352
2	12.07309174289078	10.995747015745096
3	3.9670712886331096	5.419603347533784
4	1.787343246884289	3.255698638190638
5	1.0067038749252606	2.292176139340342
6	0.6344711695687452	1.7335620281697737
7	0.4316297897209843	1.3758962705299358
8	0.3344780150005247	1.2185232129756824
9	0.24542720073825666	1.005869051943762
>10	1.515171438712053	13.028088868982552
>50	0.16441207600736113	5.097991282164652
>100	0.11598524634701113	9.544201996665636
>500	0.010163655607727778	3.0969105919691815
>1k	0.004782896756577778	3.425523434563186
>5k	0.0011957241891444445	3.123319411962237
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6098	1.6544711145839268	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5374	1.4580399753647135	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2383	0.6465406143085434	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2350	0.6375872612778334	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1432	0.38852125878717336	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1410	0.3825523567667001	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1372	0.3722424350949734	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1346	0.3651882781616867	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1217	0.3301888072234567	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1072	0.29084831663397337	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	958	0.25991855161879335	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	955	0.2591046104341833	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	826	0.22410513949595334	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	798	0.21650835510626	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	740	0.20077215887046668	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	715	0.19398931566538336	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	695	0.18856304110131666	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	639	0.17336947232193003	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	637	0.17282684486552335	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	578	0.1568193349015267	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	577	0.15654802117332337	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	565	0.15329225643488334	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	563	0.1527496289784767	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	552	0.14976517796824002	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	546	0.14813729559902	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	530	0.14379627594776667	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	501	0.13592817782987002	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	496	0.13457160918885336	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	463	0.12561825615814334	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	451	0.12236249141970333	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	411	0.11150994229157	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	408	0.11069600110696	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	400	0.10852549128133335	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	393	0.10662629518391001	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	391	0.10608366772750334	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	378	0.10255658926086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.713137282033334E-4	0.0
10	0.0	0.0	0.0	2.713137282033334E-4	0.0
11	0.0	0.0	0.0	2.713137282033334E-4	0.0
12	0.0	0.0	0.0	2.713137282033334E-4	0.0
13	0.0	0.0	0.0	5.426274564066668E-4	0.0
14	0.0	0.0	0.0	5.426274564066668E-4	0.0
15	0.0	0.0	0.0	8.139411846100001E-4	0.0
16	0.0	0.0	0.0	0.0010852549128133335	0.0
17	0.0	0.0	0.0	0.0013565686410166669	0.0
18	0.0	0.0	0.0	0.0013565686410166669	0.0
19	0.0	0.0	0.0	0.002170509825626667	0.0
20	0.0	0.0	0.0	0.0027131372820333338	0.0
21	0.0	0.0	0.0	0.004341019651253334	0.0
22	2.713137282033334E-4	0.0	0.0	0.006511529476880001	0.0
23	2.713137282033334E-4	0.0	0.0	0.014650941322980001	0.0
24	2.713137282033334E-4	0.0	0.0	0.021162470799860004	0.0
25	2.713137282033334E-4	0.0	0.0	0.027131372820333337	0.0
26	2.713137282033334E-4	0.0	0.0	0.03310027484080667	0.0
27	2.713137282033334E-4	0.0	0.0	0.04612333379456667	0.0
28	2.713137282033334E-4	0.0	0.0	0.06348741239958	0.0
29	2.713137282033334E-4	0.0	0.0	0.08654907929686334	0.0
30	2.713137282033334E-4	0.0	0.0	0.12371906006072002	0.0
31	2.713137282033334E-4	0.0	0.0	0.17906706061420002	0.0
32	2.713137282033334E-4	0.0	0.0	0.27104241447513	0.0
33	2.713137282033334E-4	0.0	0.0	0.34375449363362337	0.0
34	2.713137282033334E-4	0.0	0.0	0.46231859285848004	0.0
35	2.713137282033334E-4	0.0	0.0	0.56568912330395	0.0
36	2.713137282033334E-4	0.0	0.0	0.7347175759746267	0.0
37	2.713137282033334E-4	0.0	0.0	0.9987058335164701	0.0
38	2.713137282033334E-4	0.0	0.0	1.20788871796124	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGATT	20	7.849514E-4	44.0	41
CTAGGAC	55	7.6397555E-11	40.0	3
CTCTAGG	65	1.0913936E-11	37.230766	1
TCGTATA	60	1.9645086E-10	36.666664	44
TCCTATA	55	3.4106051E-9	36.0	2
TCTCGTA	25	0.0023506787	35.2	42
TACTCCC	25	0.0023506787	35.2	5
CCTAGAG	55	1.3693352E-7	32.0	3
CTCGTAT	35	3.213557E-4	31.428572	43
GTTTTGC	35	3.213557E-4	31.428572	31
GAACTCG	50	2.3555767E-6	30.8	31
CTAGAGT	65	1.7400453E-8	30.461536	4
GGTACAA	30	0.0057399003	29.333332	21
AAGGTAC	30	0.0057399003	29.333332	19
GATGATA	30	0.0057399003	29.333332	44
CGAGAAT	30	0.0057399003	29.333332	21
TAGGACC	385	0.0	29.142857	4
GTCCTAG	70	3.576497E-8	28.285715	1
CTCGGCA	55	4.948779E-6	28.0	34
AGGACCT	595	0.0	26.62185	5
>>END_MODULE